Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm

Abstract Background BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178...

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Autores: Montesinos Barreda, Laura, Bundó, Mireia, Badosa Romañó, Esther, San Segundo de los Mozos, Blanca, Coca, María, Montesinos Seguí, Emilio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/13755
Acceso en línea:http://hdl.handle.net/10256/13755
Access Level:acceso abierto
Palabra clave:Plantes transgèniques
Arròs -- Genètica
Transgenic plants
Rice -- Genetics
Antibiòtics pèptids
Peptide antibiotics
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spelling Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endospermMontesinos Barreda, LauraBundó, MireiaBadosa Romañó, EstherSan Segundo de los Mozos, BlancaCoca, MaríaMontesinos Seguí, EmilioPlantes transgèniquesArròs -- GenèticaTransgenic plantsRice -- GeneticsAntibiòtics pèptidsPeptide antibioticsAbstract Background BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories. Results A synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter. Among the three endosperm-specific rice promoters (Glutelin B1, Glutelin B4 or Globulin 1), best results were obtained when using the Globulin 1 promoter. The BP178 peptide accumulated in the seed endosperm and was easily recovered from rice seeds using a simple procedure with a yield of 21 μg/g. The transgene was stably inherited for at least three generations, and peptide accumulation remained stable during long term storage of transgenic seeds. The purified peptide showed in vitro activity against the bacterial plant pathogen Dickeya sp., the causal agent of the dark brown sheath rot of rice. Seedlings of transgenic events showed enhanced resistance to the fungal pathogen Fusarium verticillioides, supporting that the in planta produced peptide was biologically active. Conclusions The strategy developed in this work for the sustainable production of BP178 peptide using rice seeds as biofactories represents a promising system for future production of peptides for plant protection and possibly in other fields.BioMed Central2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/13755Bmc Plant Biology, 2017, vol. 17, núm. art.63Articles publicats (D-EQATA)Montesinos Barreda, Laura Bundó, Mireia Badosa Romañó, Esther San Segundo de los Mozos, Blanca Coca, María Montesinos Seguí, Emilio 2017 Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm Bmc Plant Biology 17 art.63reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-017-1011-9info:eu-repo/semantics/altIdentifier/issn/1471-2229Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/137552026-05-29T05:05:01Z
dc.title.none.fl_str_mv Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
title Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
spellingShingle Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
Montesinos Barreda, Laura
Plantes transgèniques
Arròs -- Genètica
Transgenic plants
Rice -- Genetics
Antibiòtics pèptids
Peptide antibiotics
title_short Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
title_full Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
title_fullStr Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
title_full_unstemmed Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
title_sort Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
dc.creator.none.fl_str_mv Montesinos Barreda, Laura
Bundó, Mireia
Badosa Romañó, Esther
San Segundo de los Mozos, Blanca
Coca, María
Montesinos Seguí, Emilio
author Montesinos Barreda, Laura
author_facet Montesinos Barreda, Laura
Bundó, Mireia
Badosa Romañó, Esther
San Segundo de los Mozos, Blanca
Coca, María
Montesinos Seguí, Emilio
author_role author
author2 Bundó, Mireia
Badosa Romañó, Esther
San Segundo de los Mozos, Blanca
Coca, María
Montesinos Seguí, Emilio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Plantes transgèniques
Arròs -- Genètica
Transgenic plants
Rice -- Genetics
Antibiòtics pèptids
Peptide antibiotics
topic Plantes transgèniques
Arròs -- Genètica
Transgenic plants
Rice -- Genetics
Antibiòtics pèptids
Peptide antibiotics
description Abstract Background BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories. Results A synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter. Among the three endosperm-specific rice promoters (Glutelin B1, Glutelin B4 or Globulin 1), best results were obtained when using the Globulin 1 promoter. The BP178 peptide accumulated in the seed endosperm and was easily recovered from rice seeds using a simple procedure with a yield of 21 μg/g. The transgene was stably inherited for at least three generations, and peptide accumulation remained stable during long term storage of transgenic seeds. The purified peptide showed in vitro activity against the bacterial plant pathogen Dickeya sp., the causal agent of the dark brown sheath rot of rice. Seedlings of transgenic events showed enhanced resistance to the fungal pathogen Fusarium verticillioides, supporting that the in planta produced peptide was biologically active. Conclusions The strategy developed in this work for the sustainable production of BP178 peptide using rice seeds as biofactories represents a promising system for future production of peptides for plant protection and possibly in other fields.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/13755
url http://hdl.handle.net/10256/13755
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-017-1011-9
info:eu-repo/semantics/altIdentifier/issn/1471-2229
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Bmc Plant Biology, 2017, vol. 17, núm. art.63
Articles publicats (D-EQATA)
Montesinos Barreda, Laura Bundó, Mireia Badosa Romañó, Esther San Segundo de los Mozos, Blanca Coca, María Montesinos Seguí, Emilio 2017 Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm Bmc Plant Biology 17 art.63
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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