Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays

medRxiv: https://doi.org/10.1101/2020.09.07.20188151

Detalles Bibliográficos
Autores: Valdivia, Arantxa, Torres, Ignacio, Latorre, Victor, Francés-Gómez, Clara, Albert, Eliseo, Gozalbo-Rovira, Roberto, Alcaraz, María Jesús, Buesa, Javier, Rodríguez-Díaz, Jesús, Geller, Ron, Navarro, David
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/220074
Acceso en línea:http://hdl.handle.net/10261/220074
Access Level:acceso abierto
Palabra clave:SARS-CoV-2
COVID-19
Neutralizing antibodies
Enzyme-linked immunosorbent assay
Chemiluminescent immunoassays
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spelling Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassaysValdivia, ArantxaTorres, IgnacioLatorre, VictorFrancés-Gómez, ClaraAlbert, EliseoGozalbo-Rovira, RobertoAlcaraz, María JesúsBuesa, JavierRodríguez-Díaz, JesúsGeller, RonNavarro, DavidSARS-CoV-2COVID-19Neutralizing antibodiesEnzyme-linked immunosorbent assayChemiluminescent immunoassaysmedRxiv: https://doi.org/10.1101/2020.09.07.20188151[Background]: Whether antibody levels measured by commercially-available enzyme or chemiluminescent immunoassays targeting the SARS-CoV-2 spike (S) protein can act as a proxy for serum neutralizing activity remains to be established for many of these assays.[Objectives]: To evaluate the degree of correlation between neutralizing antibodies (NtAb) binding the SARS-CoV-2 Spike (S) protein and SARS-CoV-2-S-IgG levels measured by four commercial immunoassays in sera drawn from hospitalized COVID-19 patients.[Patients and Methods]: Ninety sera from 51 hospitalized COVID-19 patients were assayed by a pseudotyped virus neutralization assay, the LIAISON SARS-CoV-2 S1/S2 IgG, the Euroimmun SARS-CoV-2 IgG ELISA, the MAGLUMI 2019-nCoV IgG and the COVID-19 ELISA IgG assays.[Results]: Overall, the results obtained with the COVID-19 ELISA IgG test showed the highest agreement with the NtAb assay (κ, 0.85; 95% CI, 0.63-1). The most sensitive tests were the pseudotyped virus NtAb assay and the COVID-19 ELISA IgG assay (92.2% for both). Overall, the degree correlation between antibody titers resulting in 50% virus neutralization (NtAb50) in the pseudotyped virus assay and SARS-CoV-2 IgG levels was strong for the Euroimmun SARS-CoV-2 IgG ELISA (Rho=0.73) and moderate for the remaining assays (Rho=0.48 to 0.59). The kinetic profile of serum NtAb50 titers could not be reliably predicted by any of the SARS-CoV-2 IgG immunoassays.[Conclusions]: the suitability of SARS-CoV-2-S-IgG commercial immunoassays for inferring neutralizing activity of sera from hospitalized COVID-19 patients varies widely across tests and is influenced by the time of sera collection after the onset of symptoms.This work was supported by Valencian Government grant IDIFEDER/2018/056 to JRD and Covid_19-SCI to RG.NoSpringer NatureInstituto de Salud Carlos IIIMinisterio de Economía y Competitividad (España)Generalitat ValencianaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/220074reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1007/s10096-020-04128-8Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2200742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
title Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
spellingShingle Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
Valdivia, Arantxa
SARS-CoV-2
COVID-19
Neutralizing antibodies
Enzyme-linked immunosorbent assay
Chemiluminescent immunoassays
title_short Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
title_full Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
title_fullStr Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
title_full_unstemmed Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
title_sort Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
dc.creator.none.fl_str_mv Valdivia, Arantxa
Torres, Ignacio
Latorre, Victor
Francés-Gómez, Clara
Albert, Eliseo
Gozalbo-Rovira, Roberto
Alcaraz, María Jesús
Buesa, Javier
Rodríguez-Díaz, Jesús
Geller, Ron
Navarro, David
author Valdivia, Arantxa
author_facet Valdivia, Arantxa
Torres, Ignacio
Latorre, Victor
Francés-Gómez, Clara
Albert, Eliseo
Gozalbo-Rovira, Roberto
Alcaraz, María Jesús
Buesa, Javier
Rodríguez-Díaz, Jesús
Geller, Ron
Navarro, David
author_role author
author2 Torres, Ignacio
Latorre, Victor
Francés-Gómez, Clara
Albert, Eliseo
Gozalbo-Rovira, Roberto
Alcaraz, María Jesús
Buesa, Javier
Rodríguez-Díaz, Jesús
Geller, Ron
Navarro, David
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Ministerio de Economía y Competitividad (España)
Generalitat Valenciana
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv SARS-CoV-2
COVID-19
Neutralizing antibodies
Enzyme-linked immunosorbent assay
Chemiluminescent immunoassays
topic SARS-CoV-2
COVID-19
Neutralizing antibodies
Enzyme-linked immunosorbent assay
Chemiluminescent immunoassays
description medRxiv: https://doi.org/10.1101/2020.09.07.20188151
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/220074
url http://hdl.handle.net/10261/220074
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1007/s10096-020-04128-8
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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