Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells

© 2020 by the authors.

Detalles Bibliográficos
Autores: Montero-Montero, Lucía, Renart, Jaime, Ramírez, Andrés, Ramos, Carmen, Shamhood, Mariam, Jarcovsky, Rocío, Quintanilla, Miguel, Martín-Villar, Ester
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/226727
Acceso en línea:http://hdl.handle.net/10261/226727
Access Level:acceso abierto
Palabra clave:Podoplanin
CD44s
CD44v
Squamous carcinoma cells
Protein–protein interaction
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spelling Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma CellsMontero-Montero, LucíaRenart, JaimeRamírez, AndrésRamos, CarmenShamhood, MariamJarcovsky, RocíoQuintanilla, MiguelMartín-Villar, EsterPodoplaninCD44sCD44vSquamous carcinoma cellsProtein–protein interaction© 2020 by the authors.Podoplanin and CD44 are transmembrane glycoproteins involved in inflammation and cancer. In this paper, we report that podoplanin is coordinately expressed with the CD44 standard (CD44s) and variant (CD44v) isoforms in vivo—in hyperplastic skin after a pro-inflammatory stimulus with 12-O-tetradecanoylphorbol-13-acetate (TPA)—and in vitro—in cell lines representative of different stages of mouse-skin chemical carcinogenesis, as well as in human squamous carcinoma cell (SCC) lines. Moreover, we identify CD44v10 in the mouse-skin carcinogenesis model as the only CD44 variant isoform expressed in highly aggressive spindle carcinoma cell lines together with CD44s and podoplanin. We also characterized CD44v3-10, CD44v6-10 and CD44v8-10 as the major variant isoforms co-expressed with CD44s and podoplanin in human SCC cell lines. Immunofluorescence confocal microscopy experiments show that these CD44v isoforms colocalize with podoplanin at plasma membrane protrusions and cell–cell contacts of SCC cells, as previously reported for CD44s. Furthermore, CD44v isoforms colocalize with podoplanin in chemically induced mouse-skin SCCs in vivo. Co-immunoprecipitation experiments indicate that podoplanin physically binds to CD44v3-10, CD44v6-10 and CD44v8-10 isoforms, as well as to CD44s. Podoplanin–CD44 interaction is mediated by the transmembrane and cytosolic regions and is negatively modulated by glycosylation of the extracellular domain. These results point to a functional interplay of podoplanin with both CD44v and CD44s isoforms in SCCs and give insight into the regulation of the podoplanin–CD44 association.This research was funded by a grant from Ministerio de Ciencia, Innovación y Universidades of Spain (SAF2017-84183-R) and from Fundación Universidad Francisco de Vitoria (UFV2020-28)Multidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Universidad Francisco de VitoriaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/226727reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-84183-RSAF2017-84183-R/AEI/10.13039/501100011033http://dx.doi.org/10.3390/cells9102200Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2267272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
title Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
spellingShingle Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
Montero-Montero, Lucía
Podoplanin
CD44s
CD44v
Squamous carcinoma cells
Protein–protein interaction
title_short Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
title_full Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
title_fullStr Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
title_full_unstemmed Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
title_sort Interplay between Podoplanin, CD44s and CD44v in Squamous Carcinoma Cells
dc.creator.none.fl_str_mv Montero-Montero, Lucía
Renart, Jaime
Ramírez, Andrés
Ramos, Carmen
Shamhood, Mariam
Jarcovsky, Rocío
Quintanilla, Miguel
Martín-Villar, Ester
author Montero-Montero, Lucía
author_facet Montero-Montero, Lucía
Renart, Jaime
Ramírez, Andrés
Ramos, Carmen
Shamhood, Mariam
Jarcovsky, Rocío
Quintanilla, Miguel
Martín-Villar, Ester
author_role author
author2 Renart, Jaime
Ramírez, Andrés
Ramos, Carmen
Shamhood, Mariam
Jarcovsky, Rocío
Quintanilla, Miguel
Martín-Villar, Ester
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Universidad Francisco de Vitoria
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Podoplanin
CD44s
CD44v
Squamous carcinoma cells
Protein–protein interaction
topic Podoplanin
CD44s
CD44v
Squamous carcinoma cells
Protein–protein interaction
description © 2020 by the authors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/226727
url http://hdl.handle.net/10261/226727
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-84183-R
SAF2017-84183-R/AEI/10.13039/501100011033
http://dx.doi.org/10.3390/cells9102200
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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