Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis

In adult lizards, new neurons are generated from neural stem cells in the ventricular zone of the lateral ventricles. These new neurons migrate and integrate into the main telencephalic subdivisions. In this work we have studied adult neurogenesis in the lizard Podarcis liolepis (formerly Podarcis h...

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Autores: González-Granero, Susana, Font, Enrique, Desfilis, Ester, Herranz-Pérez, Vicente, García-Verdugo, José Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/464461
Acceso en línea:https://doi.org/10.3389/fnins.2023.1125999
https://hdl.handle.net/10459.1/464461
Access Level:acceso abierto
Palabra clave:Adult neurogenesis
Reptiles
Lizard
Neural stem cells
Neuroblast migration
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spelling Adult neurogenesis in the telencephalon of the lizard Podarcis liolepisGonzález-Granero, SusanaFont, EnriqueDesfilis, EsterHerranz-Pérez, VicenteGarcía-Verdugo, José ManuelAdult neurogenesisReptilesLizardNeural stem cellsNeuroblast migrationIn adult lizards, new neurons are generated from neural stem cells in the ventricular zone of the lateral ventricles. These new neurons migrate and integrate into the main telencephalic subdivisions. In this work we have studied adult neurogenesis in the lizard Podarcis liolepis (formerly Podarcis hispanica) by administering [3H]-thymidine and bromodeoxyuridine as proliferation markers and euthanizing the animals at different survival times to determine the identity of progenitor cells and to study their lineage derivatives. After short survival times, only type B cells are labeled, suggesting that they are neural stem cells. Three days after administration, some type A cells are labeled, corresponding to recently formed neuroblasts. Type A cells migrate to their final destinations, where they differentiate into mature neurons and integrate into functional circuits. Our results after long survival periods suggest that, in addition to actively dividing type B cells, there is also a type B subpopulation with low proliferative activity. We also found that new neurons incorporated into the olfactory bulb are generated both in situ, in the walls of the anterior extension of the lateral ventricle of the olfactory bulbs, but also at more caudal levels, most likely in anterior levels of the sulcus ventralis/terminalis. These cells follow a tangential migration toward the olfactory bulbs where they integrate. We hypothesized that at least part of the newly generated neurons would undergo a specialization process over time. In support of this prediction, we found two neuronal populations in the cellular layer of the medial cortex, which we named type I and II neurons. At intermediate survival times (1 month) only type II neurons were labeled with [3H]-thymidine, while at longer survival times (3, 6, or 12 months) both type I and type II neurons were labeled. This study sheds light on the ultrastructural characteristics of the ventricular zone of P. liolepis as a neurogenic niche, and adds to our knowledge of the processes whereby newly generated neurons in the adult brain migrate and integrate into their final destinations.Frontiers Media2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3389/fnins.2023.1125999https://hdl.handle.net/10459.1/464461reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a https://doi.org/10.3389/fnins.2023.1125999Frontiers in Neuroscience, 2023, vol. 17, 1125999cc-by (c) Susana González-Granero et al., 2023Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/4644612026-05-29T05:05:01Z
dc.title.none.fl_str_mv Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
title Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
spellingShingle Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
González-Granero, Susana
Adult neurogenesis
Reptiles
Lizard
Neural stem cells
Neuroblast migration
title_short Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
title_full Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
title_fullStr Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
title_full_unstemmed Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
title_sort Adult neurogenesis in the telencephalon of the lizard Podarcis liolepis
dc.creator.none.fl_str_mv González-Granero, Susana
Font, Enrique
Desfilis, Ester
Herranz-Pérez, Vicente
García-Verdugo, José Manuel
author González-Granero, Susana
author_facet González-Granero, Susana
Font, Enrique
Desfilis, Ester
Herranz-Pérez, Vicente
García-Verdugo, José Manuel
author_role author
author2 Font, Enrique
Desfilis, Ester
Herranz-Pérez, Vicente
García-Verdugo, José Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Adult neurogenesis
Reptiles
Lizard
Neural stem cells
Neuroblast migration
topic Adult neurogenesis
Reptiles
Lizard
Neural stem cells
Neuroblast migration
description In adult lizards, new neurons are generated from neural stem cells in the ventricular zone of the lateral ventricles. These new neurons migrate and integrate into the main telencephalic subdivisions. In this work we have studied adult neurogenesis in the lizard Podarcis liolepis (formerly Podarcis hispanica) by administering [3H]-thymidine and bromodeoxyuridine as proliferation markers and euthanizing the animals at different survival times to determine the identity of progenitor cells and to study their lineage derivatives. After short survival times, only type B cells are labeled, suggesting that they are neural stem cells. Three days after administration, some type A cells are labeled, corresponding to recently formed neuroblasts. Type A cells migrate to their final destinations, where they differentiate into mature neurons and integrate into functional circuits. Our results after long survival periods suggest that, in addition to actively dividing type B cells, there is also a type B subpopulation with low proliferative activity. We also found that new neurons incorporated into the olfactory bulb are generated both in situ, in the walls of the anterior extension of the lateral ventricle of the olfactory bulbs, but also at more caudal levels, most likely in anterior levels of the sulcus ventralis/terminalis. These cells follow a tangential migration toward the olfactory bulbs where they integrate. We hypothesized that at least part of the newly generated neurons would undergo a specialization process over time. In support of this prediction, we found two neuronal populations in the cellular layer of the medial cortex, which we named type I and II neurons. At intermediate survival times (1 month) only type II neurons were labeled with [3H]-thymidine, while at longer survival times (3, 6, or 12 months) both type I and type II neurons were labeled. This study sheds light on the ultrastructural characteristics of the ventricular zone of P. liolepis as a neurogenic niche, and adds to our knowledge of the processes whereby newly generated neurons in the adult brain migrate and integrate into their final destinations.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3389/fnins.2023.1125999
https://hdl.handle.net/10459.1/464461
url https://doi.org/10.3389/fnins.2023.1125999
https://hdl.handle.net/10459.1/464461
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.3389/fnins.2023.1125999
Frontiers in Neuroscience, 2023, vol. 17, 1125999
dc.rights.none.fl_str_mv cc-by (c) Susana González-Granero et al., 2023
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Susana González-Granero et al., 2023
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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