Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles

Olfactory sensory neurons (OSNs) are chemoreceptors that establish excitatory synapses within glomeruli of the olfactory bulb. OSNs undergo continuous turnover throughout life, causing the constant replacement of their synaptic contacts. Using Xenopus tadpoles as an experimental system to investigat...

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Detalles Bibliográficos
Autores: Terni, Beatrice, Pacciolla, Paolo, Masanas, Helena, Gorostiza Langa, Pablo Ignacio, Llobet Berenguer, Artur, 1972-
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/147225
Acceso en línea:https://hdl.handle.net/2445/147225
Access Level:acceso abierto
Palabra clave:Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
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spelling Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpolesTerni, BeatricePacciolla, PaoloMasanas, HelenaGorostiza Langa, Pablo IgnacioLlobet Berenguer, Artur, 1972-SinapsiOlfacteFisiologiaMetabolismeSynapsesSmellPhysiologyMetabolismOlfactory sensory neurons (OSNs) are chemoreceptors that establish excitatory synapses within glomeruli of the olfactory bulb. OSNs undergo continuous turnover throughout life, causing the constant replacement of their synaptic contacts. Using Xenopus tadpoles as an experimental system to investigate rewiring of glomerular connectivity, we show that novel OSN synapses can transfer information immediately after formation, mediating olfactory-guided behavior. Tadpoles recover the ability to detect amino acids 4 days after bilateral olfactory nerve transection. Restoration of olfactory-guided behavior depends on the efficient reinsertion of OSNs to the olfactory bulb. Presynaptic terminals of incipient synaptic contacts generate calcium transients in response to odors, triggering long lasting depolarization of olfactory glomeruli. The functionality of reconnected terminals relies on well-defined readily releasable and cytoplasmic vesicle pools. The continuous growth of non-compartmentalized axonal processes provides a vesicle reservoir to nascent release sites, which contrasts to the gradual development of cytoplasmic vesicle pools in conventional excitatory synapses. The immediate availability of fully functional synapses upon formation supports an age-independent contribution of OSNs to the generation of odor maps.Wiley2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/147225Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1002/cne.24303Journal of Comparative Neurology, 2017, vol. 525, num. 17, p. 3769-3783https://doi.org/10.1002/cne.24303(c) Wiley, 2017info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1472252026-05-27T06:46:51Z
dc.title.none.fl_str_mv Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
spellingShingle Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
Terni, Beatrice
Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
title_short Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_full Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_fullStr Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_full_unstemmed Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_sort Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
dc.creator.none.fl_str_mv Terni, Beatrice
Pacciolla, Paolo
Masanas, Helena
Gorostiza Langa, Pablo Ignacio
Llobet Berenguer, Artur, 1972-
author Terni, Beatrice
author_facet Terni, Beatrice
Pacciolla, Paolo
Masanas, Helena
Gorostiza Langa, Pablo Ignacio
Llobet Berenguer, Artur, 1972-
author_role author
author2 Pacciolla, Paolo
Masanas, Helena
Gorostiza Langa, Pablo Ignacio
Llobet Berenguer, Artur, 1972-
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
topic Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
description Olfactory sensory neurons (OSNs) are chemoreceptors that establish excitatory synapses within glomeruli of the olfactory bulb. OSNs undergo continuous turnover throughout life, causing the constant replacement of their synaptic contacts. Using Xenopus tadpoles as an experimental system to investigate rewiring of glomerular connectivity, we show that novel OSN synapses can transfer information immediately after formation, mediating olfactory-guided behavior. Tadpoles recover the ability to detect amino acids 4 days after bilateral olfactory nerve transection. Restoration of olfactory-guided behavior depends on the efficient reinsertion of OSNs to the olfactory bulb. Presynaptic terminals of incipient synaptic contacts generate calcium transients in response to odors, triggering long lasting depolarization of olfactory glomeruli. The functionality of reconnected terminals relies on well-defined readily releasable and cytoplasmic vesicle pools. The continuous growth of non-compartmentalized axonal processes provides a vesicle reservoir to nascent release sites, which contrasts to the gradual development of cytoplasmic vesicle pools in conventional excitatory synapses. The immediate availability of fully functional synapses upon formation supports an age-independent contribution of OSNs to the generation of odor maps.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/147225
url https://hdl.handle.net/2445/147225
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1002/cne.24303
Journal of Comparative Neurology, 2017, vol. 525, num. 17, p. 3769-3783
https://doi.org/10.1002/cne.24303
dc.rights.none.fl_str_mv (c) Wiley, 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Wiley, 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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