Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae

We would like to thank Dr. J. Montero (URJC) and Prof. R. Catalá (CIB-CSIC) for their help and support in the immunodetection experiments, to Prof. M. Tetriach's group and Prof. L. Muggia for hosting JE in their laboratory at Trieste University and instructing her in the isolation and culture o...

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Detalles Bibliográficos
Autores: Expósito, Joana R., Barreno, Eva, Catalá, Myriam
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
OAI Identifier:oai:burjcdigital.urjc.es:10115/24996
Acceso en línea:https://hdl.handle.net/10115/24996
Access Level:acceso abierto
Palabra clave:Enzyme activity
Immunodetection
Nitrate reductase
Nitric oxide synthase
Phycobiont
Symbiosis
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spelling Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgaeExpósito, Joana R.Barreno, EvaCatalá, MyriamEnzyme activityImmunodetectionNitrate reductaseNitric oxide synthasePhycobiontSymbiosisWe would like to thank Dr. J. Montero (URJC) and Prof. R. Catalá (CIB-CSIC) for their help and support in the immunodetection experiments, to Prof. M. Tetriach's group and Prof. L. Muggia for hosting JE in their laboratory at Trieste University and instructing her in the isolation and culture of mycobionts. We also appreciate the technical support of Ms. S. San José (URJC) and the CULTIVE facility of RedLabu URJC.NO is a multifaceted molecule, key in functions such as abiotic stress tolerance and symbioses establishment and permanence. Lichens are complex symbiotic associations of microalgae, fungi and prokaryotes that release NO under stress conditions such as dessication-rehydration cycles and the presence of xenobiotics. NO synthase (NOS) oxidises L-arginine to produce NO in animals and some aquatic microalgae, while nitrate reductase (NR) reduces nitrate to NO in plants and fungi. Inhibition studies suggest that both activities might be present in thalli. Due to its multipartner compotition, our hypothesis is that Ramalina farinacea biosynthesises NO through both oxidative (NOS) and reductive (NR) enzymatic pathways. NR activity was quantified with a method optimised for lichens using NADH or/and NADPH, and NOS with a commercial kit in R. farinacea thalli and cultures of the isolated main symbionts: R. farinacea mycobiont, and Trebouxia jamesii and Trebouxia lynnae phycobionts. Inhibition studies in vitro were performed with L-NAME and tungstate. Immunodetection was carried out with specific polyclonal antibodies (anti-plant NADH-NR and anti-iNOS animal isoform). NADH-NR specific activity of R. farinacea is an order of magnitude higher than Arabidopsis thaliana’s and in the range of the chlorophyte Ulva intestinalis. R. farinacea mycobiont possesses a canonical plant-like Moco-NR, while Trebouxia phycobionts’ NR activity presents interesting peculiarities. NOS has not been immunodetected and NOS-like activity is inhibited by L-NAME only partially in T. jamesii. Despite NOS-like activity is very high in the isolated microalgae and fungus, it is strongly depressed in the holobiont. In summary, NR activity seems to be the main source of NO biosynthesis for the holobiont R. farinacea but it presents intriguing features that deserve further study.Elsevier202320232023info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10115/24996reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlosinstname:Universidad Rey Juan CarlosInglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:burjcdigital.urjc.es:10115/249962026-06-24T12:48:17Z
dc.title.none.fl_str_mv Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
title Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
spellingShingle Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
Expósito, Joana R.
Enzyme activity
Immunodetection
Nitrate reductase
Nitric oxide synthase
Phycobiont
Symbiosis
title_short Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
title_full Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
title_fullStr Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
title_full_unstemmed Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
title_sort Symbiotic interactions in the lichen Ramalina farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae
dc.creator.none.fl_str_mv Expósito, Joana R.
Barreno, Eva
Catalá, Myriam
author Expósito, Joana R.
author_facet Expósito, Joana R.
Barreno, Eva
Catalá, Myriam
author_role author
author2 Barreno, Eva
Catalá, Myriam
author2_role author
author
dc.subject.none.fl_str_mv Enzyme activity
Immunodetection
Nitrate reductase
Nitric oxide synthase
Phycobiont
Symbiosis
topic Enzyme activity
Immunodetection
Nitrate reductase
Nitric oxide synthase
Phycobiont
Symbiosis
description We would like to thank Dr. J. Montero (URJC) and Prof. R. Catalá (CIB-CSIC) for their help and support in the immunodetection experiments, to Prof. M. Tetriach's group and Prof. L. Muggia for hosting JE in their laboratory at Trieste University and instructing her in the isolation and culture of mycobionts. We also appreciate the technical support of Ms. S. San José (URJC) and the CULTIVE facility of RedLabu URJC.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10115/24996
url https://hdl.handle.net/10115/24996
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
collection BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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