Comparison of European eel sperm cryopreservation protocols with standardization as a target
[EN] The critical situation of the European eel (Anguilla anguilla) has urged the development of sperm cryopreservation protocols for reproduction in captivity and cryobanking. In the last years, two research groups have developed their own protocols in Spain and Hungary with positive results, but d...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/120142 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/120142 |
| Access Level: | acceso abierto |
| Palabra clave: | Anguilla anguilla Methanol DMSO DNA methylation Epigenetics ZOOLOGIA PRODUCCION ANIMAL |
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| dc.title.none.fl_str_mv |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
| title |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
| spellingShingle |
Comparison of European eel sperm cryopreservation protocols with standardization as a target Herranz-Jusdado, Juan Germán Anguilla anguilla Methanol DMSO DNA methylation Epigenetics ZOOLOGIA PRODUCCION ANIMAL |
| title_short |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
| title_full |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
| title_fullStr |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
| title_full_unstemmed |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
| title_sort |
Comparison of European eel sperm cryopreservation protocols with standardization as a target |
| dc.creator.none.fl_str_mv |
Herranz-Jusdado, Juan Germán MORINI, MARINA Rozenfeld, Christoffer Kása, E. Kollár, T. Depincé, A. Labbé, C. Horváth, Á. Gallego Albiach, Victor|||0000-0002-9733-0625 Pérez Igualada, Luz María|||0000-0002-6678-7446 Asturiano, Juan F.|||0000-0002-6441-5294 |
| author |
Herranz-Jusdado, Juan Germán |
| author_facet |
Herranz-Jusdado, Juan Germán MORINI, MARINA Rozenfeld, Christoffer Kása, E. Kollár, T. Depincé, A. Labbé, C. Horváth, Á. Gallego Albiach, Victor|||0000-0002-9733-0625 Pérez Igualada, Luz María|||0000-0002-6678-7446 Asturiano, Juan F.|||0000-0002-6441-5294 |
| author_role |
author |
| author2 |
MORINI, MARINA Rozenfeld, Christoffer Kása, E. Kollár, T. Depincé, A. Labbé, C. Horváth, Á. Gallego Albiach, Victor|||0000-0002-9733-0625 Pérez Igualada, Luz María|||0000-0002-6678-7446 Asturiano, Juan F.|||0000-0002-6441-5294 |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Ciencia Animal Instituto Universitario de Ciencia y Tecnología Animal Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural European Commission Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Anguilla anguilla Methanol DMSO DNA methylation Epigenetics ZOOLOGIA PRODUCCION ANIMAL |
| topic |
Anguilla anguilla Methanol DMSO DNA methylation Epigenetics ZOOLOGIA PRODUCCION ANIMAL |
| description |
[EN] The critical situation of the European eel (Anguilla anguilla) has urged the development of sperm cryopreservation protocols for reproduction in captivity and cryobanking. In the last years, two research groups have developed their own protocols in Spain and Hungary with positive results, but difficult to compare. Here, a series of experiments were conducted to test the quality of thawed sperm after using both protocols, determining which of them produce the best results and aiming for standardization. The quality of thawed sperm was assessed by studying the motility and kinetic values of thawed sperm from both cryopreservation protocols using a computer-assisted sperm analysis (CASA-Mot) system. In addition, a viability analysis was performed using flow cytometry to test if the cryoprotectants or the freezing-thawing process led to a reduction in spermatozoa survival. Furthermore, since during cryopreservation the sperm was treated with methylated cryoprotectants (DMSO or methanol) that may induce epigenetic changes in the sperm DNA (cytosine methylation) and could affect the offspring, we conducted a luminometric methylation assay (LUMA) to study the DNA methylation levels induced by both protocols. In this work, all the above-mentioned parameters were analyzed in fresh and frozen-thawed sperm samples. Our results showed that thawed sperm samples from both protocols presented lower sperm motility and velocity, and lower percentage of live cells than those shown in fresh sperm samples. Furthermore, sperm samples from the methanol based protocol showed significantly higher motility, velocity and percentage of live spermatozoa than the same sperm samples treated with the DMSO based protocol. In addition, the DMSO based protocol induced a hypomethylation of sperm DNA compared to fresh samples whereas the methanol based protocol did not alter sperm DNA methylation level. Our results indicate that the methanol based protocol is a more suitable protocol that preserves better the motility and genetic qualities of the European eel sperm. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2019-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/120142 |
| url |
https://riunet.upv.es/handle/10251/120142 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
European Commission https://doi.org/10.13039/501100000780 H2020 642893 Improved production strategies for endangered freshwater species. |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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1869411212797673472 |
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Comparison of European eel sperm cryopreservation protocols with standardization as a targetHerranz-Jusdado, Juan GermánMORINI, MARINARozenfeld, ChristofferKása, E.Kollár, T.Depincé, A.Labbé, C.Horváth, Á.Gallego Albiach, Victor|||0000-0002-9733-0625Pérez Igualada, Luz María|||0000-0002-6678-7446Asturiano, Juan F.|||0000-0002-6441-5294Anguilla anguillaMethanolDMSODNA methylationEpigeneticsZOOLOGIAPRODUCCION ANIMAL[EN] The critical situation of the European eel (Anguilla anguilla) has urged the development of sperm cryopreservation protocols for reproduction in captivity and cryobanking. In the last years, two research groups have developed their own protocols in Spain and Hungary with positive results, but difficult to compare. Here, a series of experiments were conducted to test the quality of thawed sperm after using both protocols, determining which of them produce the best results and aiming for standardization. The quality of thawed sperm was assessed by studying the motility and kinetic values of thawed sperm from both cryopreservation protocols using a computer-assisted sperm analysis (CASA-Mot) system. In addition, a viability analysis was performed using flow cytometry to test if the cryoprotectants or the freezing-thawing process led to a reduction in spermatozoa survival. Furthermore, since during cryopreservation the sperm was treated with methylated cryoprotectants (DMSO or methanol) that may induce epigenetic changes in the sperm DNA (cytosine methylation) and could affect the offspring, we conducted a luminometric methylation assay (LUMA) to study the DNA methylation levels induced by both protocols. In this work, all the above-mentioned parameters were analyzed in fresh and frozen-thawed sperm samples. Our results showed that thawed sperm samples from both protocols presented lower sperm motility and velocity, and lower percentage of live cells than those shown in fresh sperm samples. Furthermore, sperm samples from the methanol based protocol showed significantly higher motility, velocity and percentage of live spermatozoa than the same sperm samples treated with the DMSO based protocol. In addition, the DMSO based protocol induced a hypomethylation of sperm DNA compared to fresh samples whereas the methanol based protocol did not alter sperm DNA methylation level. Our results indicate that the methanol based protocol is a more suitable protocol that preserves better the motility and genetic qualities of the European eel sperm.Funded by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement N 642893 (IMPRESS), including the JGHJ and CR predoctoral contracts, the French CRB Anim project "Investissements d'avenir", ANR-11-INBS-0003, as well as the Hungarian EFOP 3.6.3.-VEKOP 16.-2017-00008 project, and by the Higher Education Institutional Excellence Program (1783-3/2018/FEKUTSRAT) awarded by the Ministry of Human Capacities within the framework of water related researches of Szent Istvan University. MM, EK, TK and AH were granted with Short Term Scientific Missions by the COST Office (COST Action FA1205: AQUAGAMETE).ElsevierDepartamento de Ciencia AnimalInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean CommissionRepositorio Institucional de la Universitat Politècnica de València Riunet20192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/120142reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 H2020 642893 Improved production strategies for endangered freshwater species.open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1201422026-06-13T07:49:27Z |
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15,300719 |