PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma
[EN]Background: Precision medicine is a promising strategy to identify biomarkers, stratify asthmatic patients according to different endotypes, and match them with the appropriate therapy. This proof-of-concept study aimed to investigate whether gene expression in peripheral blood could provide a v...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/158644 |
| Acceso en línea: | http://hdl.handle.net/10366/158644 |
| Access Level: | acceso abierto |
| Palabra clave: | PTGDR2 Aspirin exacerbated respiratory disease (AERD) Asthma Biomarker Chronic rhinosinusitis with nasal polyps (CRSwNP) Gene expression Allergy and Immunology Genetics 3207.01 Alergias 2409 Genética alergia e inmunología |
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PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthmaGarcía Sánchez, María AsunciónEstravís Sastre, MiguelMartin, Maria J.Pérez-Pazos, JacquelineMartín-García, CristinaGil Melcón, MaríaRamos González, JacintoEguiluz-Gracia, IbonTriviño, Juan CarlosIsidoro García, MaríaDávila González, Ignacio JesúsSanz Lozano, Catalina SofíaPTGDR2Aspirin exacerbated respiratory disease (AERD)AsthmaBiomarkerChronic rhinosinusitis with nasal polyps (CRSwNP)Gene expressionAllergy and ImmunologyGenetics3207.01 Alergias2409 Genéticaalergia e inmunología[EN]Background: Precision medicine is a promising strategy to identify biomarkers, stratify asthmatic patients according to different endotypes, and match them with the appropriate therapy. This proof-of-concept study aimed to investigate whether gene expression in peripheral blood could provide a valuable noninvasive approach for the molecular phenotyping of asthma. Methods: We performed whole-transcriptome RNA sequencing on peripheral blood of 30 non-atopic non-asthmatic controls and 30 asthmatic patients. A quantitative PCR (qPCR) validation study of PTGDR2 that encodes for CRTH2 receptor, expressed in cells involved in T2 inflammation, was developed in a cohort of 361 independent subjects: 94 non-asthmatic non-atopic controls, 187 asthmatic patients [including 82 with chronic rhinosinusitis with nasal polyposis (CRSwNP) and 24 with aspirin-exacerbated respiratory disease (AERD)], 52 with allergic rhinitis, and 28 with CRSwNP without asthma. Results: PTGDR2 was one of the most differentially overexpressed genes in asthmatic patients' peripheral blood (p-value 2.64 × 106). These results were confirmed by qPCR in the validation study, where PTGDR2 transcripts were significantly upregulated in asthmatic patients (p < 0.001). This upregulation was mainly detected in some subgroups such as allergic asthma, asthma with CRSwNP, AERD, eosinophilic asthma, and severe persistent asthma. PTGDR2 expression was detected in different blood cell types, and its correlation with eosinophil counts showed differences in some groups of asthmatic patients. Conclusions: We found that PTGDR2 expression levels could identify asthma patients, introduce a minimally invasive biomarker for adult asthma molecular phenotyping, and add additional information to blood eosinophils. Although further studies are required, analyzing PTGDR2 expression levels in peripheral blood of asthmatics might assist in selecting patients for treatment with specific antagonists. Keywords: PTGDR2; aspirin exacerbated respiratory disease (AERD); asthma; biomarker; chronic rhinosinusitis with nasal polyps (CRSwNP); gene expression.Instituto de Salud Carlos III; Juan Rodés contract; Junta de Castilla y LeónMDPI202420242021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/158644reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1586442026-06-07T06:28:51Z |
| dc.title.none.fl_str_mv |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma |
| title |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma |
| spellingShingle |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma García Sánchez, María Asunción PTGDR2 Aspirin exacerbated respiratory disease (AERD) Asthma Biomarker Chronic rhinosinusitis with nasal polyps (CRSwNP) Gene expression Allergy and Immunology Genetics 3207.01 Alergias 2409 Genética alergia e inmunología |
| title_short |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma |
| title_full |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma |
| title_fullStr |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma |
| title_full_unstemmed |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma |
| title_sort |
PTGDR2 expression in peripheral blood as a potential biomarker in adult patients with asthma |
| dc.creator.none.fl_str_mv |
García Sánchez, María Asunción Estravís Sastre, Miguel Martin, Maria J. Pérez-Pazos, Jacqueline Martín-García, Cristina Gil Melcón, María Ramos González, Jacinto Eguiluz-Gracia, Ibon Triviño, Juan Carlos Isidoro García, María Dávila González, Ignacio Jesús Sanz Lozano, Catalina Sofía |
| author |
García Sánchez, María Asunción |
| author_facet |
García Sánchez, María Asunción Estravís Sastre, Miguel Martin, Maria J. Pérez-Pazos, Jacqueline Martín-García, Cristina Gil Melcón, María Ramos González, Jacinto Eguiluz-Gracia, Ibon Triviño, Juan Carlos Isidoro García, María Dávila González, Ignacio Jesús Sanz Lozano, Catalina Sofía |
| author_role |
author |
| author2 |
Estravís Sastre, Miguel Martin, Maria J. Pérez-Pazos, Jacqueline Martín-García, Cristina Gil Melcón, María Ramos González, Jacinto Eguiluz-Gracia, Ibon Triviño, Juan Carlos Isidoro García, María Dávila González, Ignacio Jesús Sanz Lozano, Catalina Sofía |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
PTGDR2 Aspirin exacerbated respiratory disease (AERD) Asthma Biomarker Chronic rhinosinusitis with nasal polyps (CRSwNP) Gene expression Allergy and Immunology Genetics 3207.01 Alergias 2409 Genética alergia e inmunología |
| topic |
PTGDR2 Aspirin exacerbated respiratory disease (AERD) Asthma Biomarker Chronic rhinosinusitis with nasal polyps (CRSwNP) Gene expression Allergy and Immunology Genetics 3207.01 Alergias 2409 Genética alergia e inmunología |
| description |
[EN]Background: Precision medicine is a promising strategy to identify biomarkers, stratify asthmatic patients according to different endotypes, and match them with the appropriate therapy. This proof-of-concept study aimed to investigate whether gene expression in peripheral blood could provide a valuable noninvasive approach for the molecular phenotyping of asthma. Methods: We performed whole-transcriptome RNA sequencing on peripheral blood of 30 non-atopic non-asthmatic controls and 30 asthmatic patients. A quantitative PCR (qPCR) validation study of PTGDR2 that encodes for CRTH2 receptor, expressed in cells involved in T2 inflammation, was developed in a cohort of 361 independent subjects: 94 non-asthmatic non-atopic controls, 187 asthmatic patients [including 82 with chronic rhinosinusitis with nasal polyposis (CRSwNP) and 24 with aspirin-exacerbated respiratory disease (AERD)], 52 with allergic rhinitis, and 28 with CRSwNP without asthma. Results: PTGDR2 was one of the most differentially overexpressed genes in asthmatic patients' peripheral blood (p-value 2.64 × 106). These results were confirmed by qPCR in the validation study, where PTGDR2 transcripts were significantly upregulated in asthmatic patients (p < 0.001). This upregulation was mainly detected in some subgroups such as allergic asthma, asthma with CRSwNP, AERD, eosinophilic asthma, and severe persistent asthma. PTGDR2 expression was detected in different blood cell types, and its correlation with eosinophil counts showed differences in some groups of asthmatic patients. Conclusions: We found that PTGDR2 expression levels could identify asthma patients, introduce a minimally invasive biomarker for adult asthma molecular phenotyping, and add additional information to blood eosinophils. Although further studies are required, analyzing PTGDR2 expression levels in peripheral blood of asthmatics might assist in selecting patients for treatment with specific antagonists. Keywords: PTGDR2; aspirin exacerbated respiratory disease (AERD); asthma; biomarker; chronic rhinosinusitis with nasal polyps (CRSwNP); gene expression. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10366/158644 |
| url |
http://hdl.handle.net/10366/158644 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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MDPI |
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MDPI |
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reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca instname:Universidad de Salamanca (USAL) |
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Universidad de Salamanca (USAL) |
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GREDOS. Repositorio Institucional de la Universidad de Salamanca |
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GREDOS. Repositorio Institucional de la Universidad de Salamanca |
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