Expression profile of microRNAs in the testes of patients with Klinefelter syndrome
Klinefelter syndrome (KS) is the most common sex chromosome aneuploidy. A distinctive characteristic of KS is oligozoospermia. Despite multiple studies that have described the natural history of the degenerative process of germ cells in patients with KS, the molecular mechanisms that initiate this p...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/696426 |
| Acceso en línea: | http://hdl.handle.net/10486/696426 https://dx.doi.org/10.1038/s41598-020-68294-7 |
| Access Level: | acceso abierto |
| Palabra clave: | Klinefelter syndrome (KS) Oligozoospermia Degenerative process of germ cells MicroRNA Spermatogenesis Testicular tissue Medicina |
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Expression profile of microRNAs in the testes of patients with Klinefelter syndromeIbarra-Ramírez, MarisolCalvo-Anguiano, GeovanaLugo-Trampe, José de JesúsMartínez-de-Villarreal, Laura EliaRodríguez-Torres, DavidNistal Martín de Serrano, ManuelGonzález-Peramato Gutiérrez, María del PilarKlinefelter syndrome (KS)OligozoospermiaDegenerative process of germ cellsMicroRNASpermatogenesisTesticular tissueMedicinaKlinefelter syndrome (KS) is the most common sex chromosome aneuploidy. A distinctive characteristic of KS is oligozoospermia. Despite multiple studies that have described the natural history of the degenerative process of germ cells in patients with KS, the molecular mechanisms that initiate this process are not well characterized. MicroRNA (miRNA)-mediated post-transcriptional control mechanisms have been increasingly recognized as important regulators of spermatogenesis; however, only a few studies have evaluated the role of miRNAs in the gonadal failure of these patients. Here, we describe a differential expression profile for the miRNAs in testicular tissue samples taken from KS patients. We analysed testicular tissue samples from 4 KS patients and 5 control patients (obstructive azoospermia) through next-generation sequencing, which can provide information about the mechanisms involved in the degeneration of germ cells. A distinctive differential expression profile was identified for 166 miRNAs in the KS patients: 66 were upregulated, and 100 were downregulated. An interactome analysis was performed for 7 of the upregulated and the 20 downregulated miRNAs. The results showed that the target genes are involved in the development, proliferation, and differentiation processes of spermatogenesis, which may explain their role in the development of infertility. This is the first report of a miRNA expression profile generated from testicular tissue samples of KS patients.Nature ResearchDepartamento de Anatomía PatológicaDepartamento de Anatomía, Histología y NeurocienciaFacultad de Medicina20202020-07-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/696426https://dx.doi.org/10.1038/s41598-020-68294-7reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6964262026-06-23T12:46:27Z |
| dc.title.none.fl_str_mv |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome |
| title |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome |
| spellingShingle |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome Ibarra-Ramírez, Marisol Klinefelter syndrome (KS) Oligozoospermia Degenerative process of germ cells MicroRNA Spermatogenesis Testicular tissue Medicina |
| title_short |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome |
| title_full |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome |
| title_fullStr |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome |
| title_full_unstemmed |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome |
| title_sort |
Expression profile of microRNAs in the testes of patients with Klinefelter syndrome |
| dc.creator.none.fl_str_mv |
Ibarra-Ramírez, Marisol Calvo-Anguiano, Geovana Lugo-Trampe, José de Jesús Martínez-de-Villarreal, Laura Elia Rodríguez-Torres, David Nistal Martín de Serrano, Manuel González-Peramato Gutiérrez, María del Pilar |
| author |
Ibarra-Ramírez, Marisol |
| author_facet |
Ibarra-Ramírez, Marisol Calvo-Anguiano, Geovana Lugo-Trampe, José de Jesús Martínez-de-Villarreal, Laura Elia Rodríguez-Torres, David Nistal Martín de Serrano, Manuel González-Peramato Gutiérrez, María del Pilar |
| author_role |
author |
| author2 |
Calvo-Anguiano, Geovana Lugo-Trampe, José de Jesús Martínez-de-Villarreal, Laura Elia Rodríguez-Torres, David Nistal Martín de Serrano, Manuel González-Peramato Gutiérrez, María del Pilar |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Anatomía Patológica Departamento de Anatomía, Histología y Neurociencia Facultad de Medicina |
| dc.subject.none.fl_str_mv |
Klinefelter syndrome (KS) Oligozoospermia Degenerative process of germ cells MicroRNA Spermatogenesis Testicular tissue Medicina |
| topic |
Klinefelter syndrome (KS) Oligozoospermia Degenerative process of germ cells MicroRNA Spermatogenesis Testicular tissue Medicina |
| description |
Klinefelter syndrome (KS) is the most common sex chromosome aneuploidy. A distinctive characteristic of KS is oligozoospermia. Despite multiple studies that have described the natural history of the degenerative process of germ cells in patients with KS, the molecular mechanisms that initiate this process are not well characterized. MicroRNA (miRNA)-mediated post-transcriptional control mechanisms have been increasingly recognized as important regulators of spermatogenesis; however, only a few studies have evaluated the role of miRNAs in the gonadal failure of these patients. Here, we describe a differential expression profile for the miRNAs in testicular tissue samples taken from KS patients. We analysed testicular tissue samples from 4 KS patients and 5 control patients (obstructive azoospermia) through next-generation sequencing, which can provide information about the mechanisms involved in the degeneration of germ cells. A distinctive differential expression profile was identified for 166 miRNAs in the KS patients: 66 were upregulated, and 100 were downregulated. An interactome analysis was performed for 7 of the upregulated and the 20 downregulated miRNAs. The results showed that the target genes are involved in the development, proliferation, and differentiation processes of spermatogenesis, which may explain their role in the development of infertility. This is the first report of a miRNA expression profile generated from testicular tissue samples of KS patients. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-07-01 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10486/696426 https://dx.doi.org/10.1038/s41598-020-68294-7 |
| url |
http://hdl.handle.net/10486/696426 https://dx.doi.org/10.1038/s41598-020-68294-7 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Nature Research |
| publisher.none.fl_str_mv |
Nature Research |
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reponame:Biblos-e Archivo. Repositorio Institucional de la UAM instname:Universidad Autónoma de Madrid |
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Universidad Autónoma de Madrid |
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Biblos-e Archivo. Repositorio Institucional de la UAM |
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Biblos-e Archivo. Repositorio Institucional de la UAM |
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