PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa

The European Union (EU) is facing the recent arrival of the bacterium Xylella fastidiosa. Its fast spread has caused great alarm because of the economic impact it implies for the agroeconomy of European countries. Among its insect vectors, Philaenus spumarius has been demonstrated to transmit the ba...

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Detalles Bibliográficos
Autores: Lantero, E., Matallanas, B., Pascual, Susana, Callejas, C.
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/291183
Acceso en línea:http://hdl.handle.net/10261/291183
Access Level:acceso abierto
Palabra clave:Olea europaea
Predation
Xylella fastidiosa
Philaenus spumarius
DNA
Primers
Specificity
Sensitivity
Cytochrome oxidase subunit I
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spelling PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosaLantero, E.Matallanas, B.Pascual, SusanaCallejas, C.Olea europaeaPredationXylella fastidiosaPhilaenus spumariusDNAPrimersSpecificitySensitivityCytochrome oxidase subunit IThe European Union (EU) is facing the recent arrival of the bacterium Xylella fastidiosa. Its fast spread has caused great alarm because of the economic impact it implies for the agroeconomy of European countries. Among its insect vectors, Philaenus spumarius has been demonstrated to transmit the bacterium from infected to uninfected trees in the EU, where different measures have been established to control it. One of the proposals to manage this vector is the augmentation of natural enemy populations. However, the identification of candidate predator species is essential if such a management system is to be introduced. The present paper describes a set of species-specific primers designed to detect the presence of P. spumarius DNA in soil arthropod fauna generalist predators’ gut which can reveal candidate species for the pest’s biological control. Such primers have been proven to be a useful and reliable taxonomic tool for P. spumarius identification at any life stage, i.e., nymphs. This rapid and accurate identification is essential for control strategies designed to avoid the spread of the pest and consequently the considerable economic losses it causes in crops.Peer reviewedMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/291183reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDepartamento de Protección VegetalSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2911832026-05-22T06:33:51Z
dc.title.none.fl_str_mv PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
title PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
spellingShingle PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
Lantero, E.
Olea europaea
Predation
Xylella fastidiosa
Philaenus spumarius
DNA
Primers
Specificity
Sensitivity
Cytochrome oxidase subunit I
title_short PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
title_full PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
title_fullStr PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
title_full_unstemmed PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
title_sort PCR species-specific primers for molecular gut content analysis to determine the contribution of generalist predators to the biological control of the vector of Xylella fastidiosa
dc.creator.none.fl_str_mv Lantero, E.
Matallanas, B.
Pascual, Susana
Callejas, C.
author Lantero, E.
author_facet Lantero, E.
Matallanas, B.
Pascual, Susana
Callejas, C.
author_role author
author2 Matallanas, B.
Pascual, Susana
Callejas, C.
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Olea europaea
Predation
Xylella fastidiosa
Philaenus spumarius
DNA
Primers
Specificity
Sensitivity
Cytochrome oxidase subunit I
topic Olea europaea
Predation
Xylella fastidiosa
Philaenus spumarius
DNA
Primers
Specificity
Sensitivity
Cytochrome oxidase subunit I
description The European Union (EU) is facing the recent arrival of the bacterium Xylella fastidiosa. Its fast spread has caused great alarm because of the economic impact it implies for the agroeconomy of European countries. Among its insect vectors, Philaenus spumarius has been demonstrated to transmit the bacterium from infected to uninfected trees in the EU, where different measures have been established to control it. One of the proposals to manage this vector is the augmentation of natural enemy populations. However, the identification of candidate predator species is essential if such a management system is to be introduced. The present paper describes a set of species-specific primers designed to detect the presence of P. spumarius DNA in soil arthropod fauna generalist predators’ gut which can reveal candidate species for the pest’s biological control. Such primers have been proven to be a useful and reliable taxonomic tool for P. spumarius identification at any life stage, i.e., nymphs. This rapid and accurate identification is essential for control strategies designed to avoid the spread of the pest and consequently the considerable economic losses it causes in crops.
publishDate 2018
dc.date.none.fl_str_mv 2018
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/291183
url http://hdl.handle.net/10261/291183
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Departamento de Protección Vegetal
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411178000678912
score 15,811543

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