Effects of microtubule length and crowding on active microtubule network organization

Active filament networks can organize into various dynamic architectures driven by cross-linking motors. Densities and kinetic properties of motors and microtubules have been shown previously to determine active microtubule network self-organization, but the effects of other control parameters are l...

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Authors: Chew, Wei-Xiang, Henkin, Gil, Nédélec, François, Surrey, Thomas
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Universitat Pompeu Fabra
Repository:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/56249
Online Access:http://hdl.handle.net/10230/56249
http://dx.doi.org/10.1016/j.isci.2023.106063
Access Level:Open access
Keyword:Biological sciences
Biophysics
Cell biology
Functional aspects of cell biology
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spelling Effects of microtubule length and crowding on active microtubule network organizationChew, Wei-XiangHenkin, GilNédélec, FrançoisSurrey, ThomasBiological sciencesBiophysicsCell biologyFunctional aspects of cell biologyActive filament networks can organize into various dynamic architectures driven by cross-linking motors. Densities and kinetic properties of motors and microtubules have been shown previously to determine active microtubule network self-organization, but the effects of other control parameters are less understood. Using computer simulations, we study here how microtubule lengths and crowding effects determine active network architecture and dynamics. We find that attractive interactions mimicking crowding effects or long microtubules both promote the formation of extensile nematic networks instead of asters. When microtubules are very long and the network is highly connected, a new isotropically motile network state resembling a "gliding mesh" is predicted. Using in vitro reconstitutions, we confirm the existence of this gliding mesh experimentally. These results provide a better understanding of how active microtubule network organization can be controlled, with implications for cell biology and active materials in general.This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness to the CRG-EMBL partnership, the Centro de Excelencia Severo Ochoa, and the CERCA Program of the Generalitat de Catalunya. W.-X.C. is supported by a Human Frontier Science Program fellowship (HFSP LT000682/2020-C). F.N. is supported by the Gatsby Charitable Foundation (Grant PTAG-024). F.N. and T.S. acknowledge support from the European Research Council (ERC Synergy Grant, Project 951430).Elsevier202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/56249http://dx.doi.org/10.1016/j.isci.2023.106063reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésiScience. 2023 Jan 27;26(2):106063info:eu-repo/grantAgreement/EC/H2020/951430© 2023 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/562492026-06-12T07:21:37Z
dc.title.none.fl_str_mv Effects of microtubule length and crowding on active microtubule network organization
title Effects of microtubule length and crowding on active microtubule network organization
spellingShingle Effects of microtubule length and crowding on active microtubule network organization
Chew, Wei-Xiang
Biological sciences
Biophysics
Cell biology
Functional aspects of cell biology
title_short Effects of microtubule length and crowding on active microtubule network organization
title_full Effects of microtubule length and crowding on active microtubule network organization
title_fullStr Effects of microtubule length and crowding on active microtubule network organization
title_full_unstemmed Effects of microtubule length and crowding on active microtubule network organization
title_sort Effects of microtubule length and crowding on active microtubule network organization
dc.creator.none.fl_str_mv Chew, Wei-Xiang
Henkin, Gil
Nédélec, François
Surrey, Thomas
author Chew, Wei-Xiang
author_facet Chew, Wei-Xiang
Henkin, Gil
Nédélec, François
Surrey, Thomas
author_role author
author2 Henkin, Gil
Nédélec, François
Surrey, Thomas
author2_role author
author
author
dc.subject.none.fl_str_mv Biological sciences
Biophysics
Cell biology
Functional aspects of cell biology
topic Biological sciences
Biophysics
Cell biology
Functional aspects of cell biology
description Active filament networks can organize into various dynamic architectures driven by cross-linking motors. Densities and kinetic properties of motors and microtubules have been shown previously to determine active microtubule network self-organization, but the effects of other control parameters are less understood. Using computer simulations, we study here how microtubule lengths and crowding effects determine active network architecture and dynamics. We find that attractive interactions mimicking crowding effects or long microtubules both promote the formation of extensile nematic networks instead of asters. When microtubules are very long and the network is highly connected, a new isotropically motile network state resembling a "gliding mesh" is predicted. Using in vitro reconstitutions, we confirm the existence of this gliding mesh experimentally. These results provide a better understanding of how active microtubule network organization can be controlled, with implications for cell biology and active materials in general.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/56249
http://dx.doi.org/10.1016/j.isci.2023.106063
url http://hdl.handle.net/10230/56249
http://dx.doi.org/10.1016/j.isci.2023.106063
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv iScience. 2023 Jan 27;26(2):106063
info:eu-repo/grantAgreement/EC/H2020/951430
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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