Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes

The joint estuary of Tinto and Odiel rivers (SW Spain) is one of the most degraded and polluted areas in the world and its recovery is mandatory. Legumes and their associated bacteria are recommended sustainable tools to fight against soils degradation and loss of fertility due to their known positi...

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Autores: Flores Duarte, Noris J., Caballero Delgado, Sara, Pajuelo Domínguez, Eloísa, Mateos Naranjo, Enrique, Redondo Gómez, Susana, Navarro Torre, Salvadora, Rodríguez Llorente, Ignacio David
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/146474
Acesso em linha:https://hdl.handle.net/11441/146474
https://doi.org/10.3389/fmicb.2022.1005458
Access Level:acceso abierto
Palavra-chave:Contaminated estuarine soils
Enhanced nodulation
Nodule endophytes
Phytostabilization
Plant growth-promoting bacteria
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spelling Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytesFlores Duarte, Noris J.Caballero Delgado, SaraPajuelo Domínguez, EloísaMateos Naranjo, EnriqueRedondo Gómez, SusanaNavarro Torre, SalvadoraRodríguez Llorente, Ignacio DavidContaminated estuarine soilsEnhanced nodulationNodule endophytesPhytostabilizationPlant growth-promoting bacteriaThe joint estuary of Tinto and Odiel rivers (SW Spain) is one of the most degraded and polluted areas in the world and its recovery is mandatory. Legumes and their associated bacteria are recommended sustainable tools to fight against soils degradation and loss of fertility due to their known positive impacts on soils. The aim of this work was to isolate and characterize plant growth promoting nodule endophytes (PGPNE) from inside nodules of Medicago spp. naturally growing in the estuary of the Tinto and Odiel Rivers and evaluate their ability to promote legume adaptation in degraded soils. The best rhizobia and non-rhizobia among 33 endophytes were selected based on their plant growth promoting properties and bacterial enzymatic activities. These strains, identified as Pseudomonas sp. N4, Pseudomonas sp. N8, Ensifer sp. N10 and Ensifer sp. N12, were used for in vitro studies using Medicago sativa plants. The effects of individual or combined inoculation on seed germination, plant growth and nodulation were studied, both on plates and pots containing nutrient-poor soils and moderately contaminated with metals/loids from the estuary. In general, inoculation with combinations of rhizobia and Pseudomonas increased plant biomass (up to 1.5-fold) and nodules number (up to 2-fold) compared to single inoculation with rhizobia, ameliorating the physiological state of the plants and helping to regulate plant stress mechanisms. The greatest benefits were observed in plants inoculated with the consortium containing the four strains. In addition, combined inoculation with Ensifer and Pseudomonas increased As and metals accumulation in plant roots, without significant differences in shoot metal accumulation. These results suggest that PGPNE are useful biotools to promote legume growth and phytostabilization potential in nutrient-poor and/or metals contaminated estuarine soils.Ministerio de Ciencia e Innovación PDC2021-120951-I00Junta de Andalucía US-1262036, PAIDI2020, P20_00682Frontiers Media S.A.Microbiología y ParasitologíaBiología Vegetal y EcologíaMinisterio de Ciencia e Innovación (MICIN). EspañaJunta de Andalucía2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/146474https://doi.org/10.3389/fmicb.2022.1005458reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésFrontiers in Microbiology, 13.PDC2021-120951-I00US-1262036PAIDI2020P20_00682https://doi.org/10.3389/fmicb.2022.1005458info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1464742026-06-17T12:51:07Z
dc.title.none.fl_str_mv Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
title Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
spellingShingle Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
Flores Duarte, Noris J.
Contaminated estuarine soils
Enhanced nodulation
Nodule endophytes
Phytostabilization
Plant growth-promoting bacteria
title_short Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
title_full Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
title_fullStr Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
title_full_unstemmed Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
title_sort Enhanced legume growth and adaptation to degraded estuarine soils using Pseudomonas sp. nodule endophytes
dc.creator.none.fl_str_mv Flores Duarte, Noris J.
Caballero Delgado, Sara
Pajuelo Domínguez, Eloísa
Mateos Naranjo, Enrique
Redondo Gómez, Susana
Navarro Torre, Salvadora
Rodríguez Llorente, Ignacio David
author Flores Duarte, Noris J.
author_facet Flores Duarte, Noris J.
Caballero Delgado, Sara
Pajuelo Domínguez, Eloísa
Mateos Naranjo, Enrique
Redondo Gómez, Susana
Navarro Torre, Salvadora
Rodríguez Llorente, Ignacio David
author_role author
author2 Caballero Delgado, Sara
Pajuelo Domínguez, Eloísa
Mateos Naranjo, Enrique
Redondo Gómez, Susana
Navarro Torre, Salvadora
Rodríguez Llorente, Ignacio David
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
Biología Vegetal y Ecología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
dc.subject.none.fl_str_mv Contaminated estuarine soils
Enhanced nodulation
Nodule endophytes
Phytostabilization
Plant growth-promoting bacteria
topic Contaminated estuarine soils
Enhanced nodulation
Nodule endophytes
Phytostabilization
Plant growth-promoting bacteria
description The joint estuary of Tinto and Odiel rivers (SW Spain) is one of the most degraded and polluted areas in the world and its recovery is mandatory. Legumes and their associated bacteria are recommended sustainable tools to fight against soils degradation and loss of fertility due to their known positive impacts on soils. The aim of this work was to isolate and characterize plant growth promoting nodule endophytes (PGPNE) from inside nodules of Medicago spp. naturally growing in the estuary of the Tinto and Odiel Rivers and evaluate their ability to promote legume adaptation in degraded soils. The best rhizobia and non-rhizobia among 33 endophytes were selected based on their plant growth promoting properties and bacterial enzymatic activities. These strains, identified as Pseudomonas sp. N4, Pseudomonas sp. N8, Ensifer sp. N10 and Ensifer sp. N12, were used for in vitro studies using Medicago sativa plants. The effects of individual or combined inoculation on seed germination, plant growth and nodulation were studied, both on plates and pots containing nutrient-poor soils and moderately contaminated with metals/loids from the estuary. In general, inoculation with combinations of rhizobia and Pseudomonas increased plant biomass (up to 1.5-fold) and nodules number (up to 2-fold) compared to single inoculation with rhizobia, ameliorating the physiological state of the plants and helping to regulate plant stress mechanisms. The greatest benefits were observed in plants inoculated with the consortium containing the four strains. In addition, combined inoculation with Ensifer and Pseudomonas increased As and metals accumulation in plant roots, without significant differences in shoot metal accumulation. These results suggest that PGPNE are useful biotools to promote legume growth and phytostabilization potential in nutrient-poor and/or metals contaminated estuarine soils.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/146474
https://doi.org/10.3389/fmicb.2022.1005458
url https://hdl.handle.net/11441/146474
https://doi.org/10.3389/fmicb.2022.1005458
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Microbiology, 13.
PDC2021-120951-I00
US-1262036
PAIDI2020
P20_00682
https://doi.org/10.3389/fmicb.2022.1005458
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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