ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice.
ICAP-1 regulates β1-integrin activation and cell adhesion. Here, we used ICAP-1-null mice to study ICAP-1 potential involvement during immune cell development and function. Integrin α4β1-dependent adhesion was comparable between ICAP-1-null and control thymocytes, but lack of ICAP-1 caused a defecti...
| Autores: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/15732 |
| Acceso en línea: | http://hdl.handle.net/20.500.12105/15732 |
| Access Level: | acceso abierto |
| Palabra clave: | Adaptor Proteins, Signal Transducing B-Lymphocytes CD8-Positive T-Lymphocytes Lymphocyte Activation Thymocytes Animals Cell Differentiation Integrin beta1 Mice Mice, Knockout Spleen Thymus Gland |
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ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice.Sevilla-Movilla, SilviaFuentes, PatriciaRodríguez-García, YaizaArellano-Sánchez, NohemiKrenn, Peter Wde Val, Soledad IsernMontero-Herradón, SaraGarcía-Ceca, JavierBurdiel-Herencia, ValeriaGardeta, Sofía RAguilera-Montilla, NoemíBarrio-Alonso, CeliaCrainiciuc, GeorgianaBouvard, DanielGarcía-Pardo, AngelesZapata, Agustin GHidalgo, AndresFässler, ReinhardCarrasco, Yolanda RToribio, Maria LTeixidó, JoaquinAdaptor Proteins, Signal TransducingB-LymphocytesCD8-Positive T-LymphocytesLymphocyte ActivationThymocytesAnimalsCell DifferentiationIntegrin beta1MiceMice, KnockoutSpleenThymus GlandICAP-1 regulates β1-integrin activation and cell adhesion. Here, we used ICAP-1-null mice to study ICAP-1 potential involvement during immune cell development and function. Integrin α4β1-dependent adhesion was comparable between ICAP-1-null and control thymocytes, but lack of ICAP-1 caused a defective single-positive (SP) CD8+ cell generation, thus, unveiling an ICAP-1 involvement in SP thymocyte development. ICAP-1 bears a nuclear localization signal and we found it displayed a strong nuclear distribution in thymocytes. Interestingly, there was a direct correlation between the lack of ICAP-1 and reduced levels in SP CD8+ thymocytes of Runx3, a transcription factor required for CD8+ thymocyte generation. In the spleen, ICAP-1 was found evenly distributed between cytoplasm and nuclear fractions, and ICAP-1-/- spleen T and B cells displayed upregulation of α4β1-mediated adhesion, indicating that ICAP-1 negatively controls their attachment. Furthermore, CD3+ - and CD19+ -selected spleen cells from ICAP-1-null mice showed reduced proliferation in response to T- and B-cell stimuli, respectively. Finally, loss of ICAP-1 caused a remarkable decrease in marginal zone B- cell frequencies and a moderate increase in follicular B cells. Together, these data unravel an ICAP-1 involvement in the generation of SP CD8+ thymocytes and in the control of marginal zone B-cell numbers.WileyMinisterio de Ciencia e Innovación (España)Instituto de Salud Carlos IIIUnión Europea. Comisión Europea. European Research Council (ERC)20232023-04-0320222022-08-0120222022-08-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/15732reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/157322026-06-12T12:43:37Z |
| dc.title.none.fl_str_mv |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. |
| title |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. |
| spellingShingle |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. Sevilla-Movilla, Silvia Adaptor Proteins, Signal Transducing B-Lymphocytes CD8-Positive T-Lymphocytes Lymphocyte Activation Thymocytes Animals Cell Differentiation Integrin beta1 Mice Mice, Knockout Spleen Thymus Gland |
| title_short |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. |
| title_full |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. |
| title_fullStr |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. |
| title_full_unstemmed |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. |
| title_sort |
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice. |
| dc.creator.none.fl_str_mv |
Sevilla-Movilla, Silvia Fuentes, Patricia Rodríguez-García, Yaiza Arellano-Sánchez, Nohemi Krenn, Peter W de Val, Soledad Isern Montero-Herradón, Sara García-Ceca, Javier Burdiel-Herencia, Valeria Gardeta, Sofía R Aguilera-Montilla, Noemí Barrio-Alonso, Celia Crainiciuc, Georgiana Bouvard, Daniel García-Pardo, Angeles Zapata, Agustin G Hidalgo, Andres Fässler, Reinhard Carrasco, Yolanda R Toribio, Maria L Teixidó, Joaquin |
| author |
Sevilla-Movilla, Silvia |
| author_facet |
Sevilla-Movilla, Silvia Fuentes, Patricia Rodríguez-García, Yaiza Arellano-Sánchez, Nohemi Krenn, Peter W de Val, Soledad Isern Montero-Herradón, Sara García-Ceca, Javier Burdiel-Herencia, Valeria Gardeta, Sofía R Aguilera-Montilla, Noemí Barrio-Alonso, Celia Crainiciuc, Georgiana Bouvard, Daniel García-Pardo, Angeles Zapata, Agustin G Hidalgo, Andres Fässler, Reinhard Carrasco, Yolanda R Toribio, Maria L Teixidó, Joaquin |
| author_role |
author |
| author2 |
Fuentes, Patricia Rodríguez-García, Yaiza Arellano-Sánchez, Nohemi Krenn, Peter W de Val, Soledad Isern Montero-Herradón, Sara García-Ceca, Javier Burdiel-Herencia, Valeria Gardeta, Sofía R Aguilera-Montilla, Noemí Barrio-Alonso, Celia Crainiciuc, Georgiana Bouvard, Daniel García-Pardo, Angeles Zapata, Agustin G Hidalgo, Andres Fässler, Reinhard Carrasco, Yolanda R Toribio, Maria L Teixidó, Joaquin |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Ciencia e Innovación (España) Instituto de Salud Carlos III Unión Europea. Comisión Europea. European Research Council (ERC) |
| dc.subject.none.fl_str_mv |
Adaptor Proteins, Signal Transducing B-Lymphocytes CD8-Positive T-Lymphocytes Lymphocyte Activation Thymocytes Animals Cell Differentiation Integrin beta1 Mice Mice, Knockout Spleen Thymus Gland |
| topic |
Adaptor Proteins, Signal Transducing B-Lymphocytes CD8-Positive T-Lymphocytes Lymphocyte Activation Thymocytes Animals Cell Differentiation Integrin beta1 Mice Mice, Knockout Spleen Thymus Gland |
| description |
ICAP-1 regulates β1-integrin activation and cell adhesion. Here, we used ICAP-1-null mice to study ICAP-1 potential involvement during immune cell development and function. Integrin α4β1-dependent adhesion was comparable between ICAP-1-null and control thymocytes, but lack of ICAP-1 caused a defective single-positive (SP) CD8+ cell generation, thus, unveiling an ICAP-1 involvement in SP thymocyte development. ICAP-1 bears a nuclear localization signal and we found it displayed a strong nuclear distribution in thymocytes. Interestingly, there was a direct correlation between the lack of ICAP-1 and reduced levels in SP CD8+ thymocytes of Runx3, a transcription factor required for CD8+ thymocyte generation. In the spleen, ICAP-1 was found evenly distributed between cytoplasm and nuclear fractions, and ICAP-1-/- spleen T and B cells displayed upregulation of α4β1-mediated adhesion, indicating that ICAP-1 negatively controls their attachment. Furthermore, CD3+ - and CD19+ -selected spleen cells from ICAP-1-null mice showed reduced proliferation in response to T- and B-cell stimuli, respectively. Finally, loss of ICAP-1 caused a remarkable decrease in marginal zone B- cell frequencies and a moderate increase in follicular B cells. Together, these data unravel an ICAP-1 involvement in the generation of SP CD8+ thymocytes and in the control of marginal zone B-cell numbers. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-08-01 2022 2022-08-01 2023 2023-04-03 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12105/15732 |
| url |
http://hdl.handle.net/20.500.12105/15732 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
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reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII) |
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Instituto de Salud Carlos III (ISCIII) |
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Repisalud |
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Repisalud |
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1869411157847048192 |
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15,81155 |