Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea

Este artículo contiene 13 páginas, 4 figuras, 2 tablas.

Detalhes bibliográficos
Autores: Viard, Frédérique, Roby, Charlotte, Turon, Xavier, Bouchemousse, Sarah, Bishop, J. D. D.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193549
Acesso em linha:http://hdl.handle.net/10261/193549
Access Level:acceso abierto
Palavra-chave:Non-native species
Cryptic species
Barcoding
COI
Marine
Tunicates
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spelling Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean SeaViard, FrédériqueRoby, CharlotteTuron, XavierBouchemousse, SarahBishop, J. D. D.Non-native speciesCryptic speciesBarcodingCOIMarineTunicatesEste artículo contiene 13 páginas, 4 figuras, 2 tablas.Molecular tools have been extensively used in recent decades to examine biological invasion processes, and are increasingly being adopted as efficient tools to support non-indigenous species surveys, notably through barcoding approaches, i.e., the use of a reference sequence specific to a given species to validate its identification. The technique is easy to use but requires reliable reference sequences to be available in public databases. In addition, the increasing discovery of cryptic species in marine taxa may complicate taxonomic assignment. We illustrate these two issues in the ascidian genus Botrylloides, in which at least three global marine invaders have been recognized, including B. violaceus and B. diegensis. We obtained COI sequences from >750 colonies of Botrylloides spp. sampled in W Europe or provided by expert colleagues from other regions. Phylogenetic trees clearly distinguished our targeted taxa [i.e., B. violaceus, B. diegensis and B. leachii (native)]. They also revealed another discrete lineage apparently related to a recently described eastern Mediterranean species. By examining public databases, we found sequences of B. diegensis erroneously assigned to B. leachii. This observation has major implications as the introduced B. diegensis can be misidentified as a putatively native species. We also checked published sequences of the genus Botrylloides in the Mediterranean Sea, complemented with new samples. Based on our custom reference database, all published sequences of B. leachii corresponded to B. diegensis, although this NIS has hardly been reported at all in the Mediterranean region. Such database errors are unfortunate, as the barcoding approach is a powerful tool to identify the recognized Botrylloides species currently present in European seas. This is of particular importance because a trait often used during field assessment, i.e., single-color vs. two-color colonies, is misleading to distinguish B. violaceus and B. diegensis respectively: a substantial proportion of the single-color morph are actually B. diegensis in both the Mediterranean Sea and the English Channel. Altogether, this study exemplifies the advantages and disadvantages of molecular barcoding in NIS surveys and studies. The limitations that were identified are all easy to resolve once proper vouchers and collections are set up.This work was part of the Interreg IVA project ‘Marinexus’ financed under the European Regional Development Fund. And also received further support from the AquaNIS2.0 project funded by the Fondation TOTAL, and from project CTM2017- 88080 (MCIU/AEI/FEDER/UE) of the Spanish Government.Peer reviewedFrontiers MediaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/193549reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3389/fmars.2019.00615Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1935492026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
title Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
spellingShingle Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
Viard, Frédérique
Non-native species
Cryptic species
Barcoding
COI
Marine
Tunicates
title_short Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
title_full Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
title_fullStr Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
title_full_unstemmed Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
title_sort Cryptic Diversity and Database Errors Challenge Non-indigenous Species Surveys: An Illustration With Botrylloides spp. in the English Channel and Mediterranean Sea
dc.creator.none.fl_str_mv Viard, Frédérique
Roby, Charlotte
Turon, Xavier
Bouchemousse, Sarah
Bishop, J. D. D.
author Viard, Frédérique
author_facet Viard, Frédérique
Roby, Charlotte
Turon, Xavier
Bouchemousse, Sarah
Bishop, J. D. D.
author_role author
author2 Roby, Charlotte
Turon, Xavier
Bouchemousse, Sarah
Bishop, J. D. D.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Non-native species
Cryptic species
Barcoding
COI
Marine
Tunicates
topic Non-native species
Cryptic species
Barcoding
COI
Marine
Tunicates
description Este artículo contiene 13 páginas, 4 figuras, 2 tablas.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193549
url http://hdl.handle.net/10261/193549
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3389/fmars.2019.00615
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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