Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth

[Background] The characteristics of pollen tube growth are not constant, but display distinct patterns of growth within the different tissues of the pistil. In the stigma, the growth rate is slow and autotrophic, whereas in the style, it is rapid and heterotrophic. Very little is known about the int...

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Detalhes bibliográficos
Autores: Losada Rodríguez, Juan Manuel, Herrero Romero, María
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/89289
Acesso em linha:http://hdl.handle.net/10261/89289
Access Level:acceso abierto
Palavra-chave:Arabinogalactan proteins
AGPs
Apple
Callose
Extensins
EXTs
Malus x domestica
Pistil
Pollen tube
Style
Transmitting tissue
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repository_id_str
spelling Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growthLosada Rodríguez, Juan ManuelHerrero Romero, MaríaArabinogalactan proteinsAGPsAppleCalloseExtensinsEXTsMalus x domesticaPistilPollen tubeStyleTransmitting tissue[Background] The characteristics of pollen tube growth are not constant, but display distinct patterns of growth within the different tissues of the pistil. In the stigma, the growth rate is slow and autotrophic, whereas in the style, it is rapid and heterotrophic. Very little is known about the interactions between these distinct maternal tissues and the traversing pollen tube and the role of this interaction on the observed metabolism. In this work we characterise pollen tube growth in the apple flower and look for differences in glycoprotein epitope localization between two different maternal tissues, the stigma and the style.[Results] While immunocytochemically-detected arabinogalactan proteins were present at high levels in the stigma, they were not detected in the transmitting tissue of the style, where extensins were abundant. Whereas extensins remained at high levels in unpollinated pistils, they were no longer present in the style following pollen tube passage. Similarily, while abundant in unpollinated styles, insoluble polysaccharides such as β-glucans, were depleted in pollinated pistils.[Conclusions] The switch from autotropic to heterotrophic pollen tube growth correlates spatially with a change of glycoprotein epitopes between the stigma and the style. The depletion of extensins and polysaccharides following pollen tube passage in the style suggest a possible contribution to the acceleration of heterotrophic pollen tube growth, which would imply an active contribution of female tissues on prezygotic male–female crosstalk.This work was supported by Ministerio de Ciencia e Innovación (MICINN)-FEDER [AGL2006-13529-C02-01, AGL 12621-C02-01, AGL 2012–40239], and Gobierno de Aragón [group A43].Peer ReviewedBioMed CentralMinisterio de Ciencia e Innovación (España)European CommissionGobierno de AragónConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2014201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/89289reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/1471-2229-14-1Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/892892026-05-22T06:33:51Z
dc.title.none.fl_str_mv Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
title Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
spellingShingle Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
Losada Rodríguez, Juan Manuel
Arabinogalactan proteins
AGPs
Apple
Callose
Extensins
EXTs
Malus x domestica
Pistil
Pollen tube
Style
Transmitting tissue
title_short Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
title_full Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
title_fullStr Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
title_full_unstemmed Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
title_sort Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth
dc.creator.none.fl_str_mv Losada Rodríguez, Juan Manuel
Herrero Romero, María
author Losada Rodríguez, Juan Manuel
author_facet Losada Rodríguez, Juan Manuel
Herrero Romero, María
author_role author
author2 Herrero Romero, María
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Gobierno de Aragón
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arabinogalactan proteins
AGPs
Apple
Callose
Extensins
EXTs
Malus x domestica
Pistil
Pollen tube
Style
Transmitting tissue
topic Arabinogalactan proteins
AGPs
Apple
Callose
Extensins
EXTs
Malus x domestica
Pistil
Pollen tube
Style
Transmitting tissue
description [Background] The characteristics of pollen tube growth are not constant, but display distinct patterns of growth within the different tissues of the pistil. In the stigma, the growth rate is slow and autotrophic, whereas in the style, it is rapid and heterotrophic. Very little is known about the interactions between these distinct maternal tissues and the traversing pollen tube and the role of this interaction on the observed metabolism. In this work we characterise pollen tube growth in the apple flower and look for differences in glycoprotein epitope localization between two different maternal tissues, the stigma and the style.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/89289
url http://hdl.handle.net/10261/89289
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1186/1471-2229-14-1
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411143302250496
score 15.812429

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