Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis

9 páginas, 4 figuras.

Detalles Bibliográficos
Autores: Suárez-Vega, Aroa, Toral, Pablo G., Gutiérrez Gil, Beatriz, Hervás, Gonzalo, Arranz, Juan José, Frutos, Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/148292
Acceso en línea:http://hdl.handle.net/10261/148292
Access Level:acceso abierto
Palabra clave:RNA-Seq
Dairy sheep
Mammary gland transcriptome
Milk fat synthesis
Nutrigenomics
Marine lipids
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spelling Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysisSuárez-Vega, AroaToral, Pablo G.Gutiérrez Gil, BeatrizHervás, GonzaloArranz, Juan JoséFrutos, PilarRNA-SeqDairy sheepMammary gland transcriptomeMilk fat synthesisNutrigenomicsMarine lipids9 páginas, 4 figuras.In this study, RNA sequencing was used to obtain a comprehensive profile of the transcriptomic changes occurring in the mammary gland of lactating sheep suffering from fish oil-induced milk fat depression (FO-MFD). The milk somatic cell transcriptome analysis of four control and four FO-MFD ewes generated an average of 42 million paired-end reads per sample. In both conditions, less than 220 genes constitute approximately 89% of the total counts. These genes, which are considered as core genes, were mainly involved in cytoplasmic ribosomal proteins and electron transport chain pathways. In total, 117 genes were upregulated, and 96 genes were downregulated in FO-MFD samples. Functional analysis of the latter indicated a downregulation of genes involved in the SREBP signaling pathway (e.g., ACACA, ACSL, and ACSS) and Gene Ontology terms related to lipid metabolism and lipid biosynthetic processes. Integrated interpretation of upregulated genes indicated enrichment in genes encoding plasma membrane proteins and proteins regulating protein kinase activity. Overall, our results indicate that FO-MFD is associated with the downregulation of key genes involved in the mammary lipogenesis process. In addition, the results also suggest that this syndrome may be related to upregulation of other genes implicated in signal transduction and codification of transcription factors.This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2014-54587-R and AGL2015-66035-R projects, co-funded by the European Regional Development Fund). P.G.T. and B.G.G. are funded by two postdoctoral research contracts from MINECO (Juan de la Cierva -JCI-2012-11836- and Ramón y Cajal -RYC-2012-10230 programs, respectively).Peer reviewedNature Publishing GroupMinisterio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/148292reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-54587-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-66035-Rhttp://dx.doi.org/10.1038/srep45905Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1482922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
title Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
spellingShingle Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
Suárez-Vega, Aroa
RNA-Seq
Dairy sheep
Mammary gland transcriptome
Milk fat synthesis
Nutrigenomics
Marine lipids
title_short Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
title_full Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
title_fullStr Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
title_full_unstemmed Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
title_sort Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis
dc.creator.none.fl_str_mv Suárez-Vega, Aroa
Toral, Pablo G.
Gutiérrez Gil, Beatriz
Hervás, Gonzalo
Arranz, Juan José
Frutos, Pilar
author Suárez-Vega, Aroa
author_facet Suárez-Vega, Aroa
Toral, Pablo G.
Gutiérrez Gil, Beatriz
Hervás, Gonzalo
Arranz, Juan José
Frutos, Pilar
author_role author
author2 Toral, Pablo G.
Gutiérrez Gil, Beatriz
Hervás, Gonzalo
Arranz, Juan José
Frutos, Pilar
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv RNA-Seq
Dairy sheep
Mammary gland transcriptome
Milk fat synthesis
Nutrigenomics
Marine lipids
topic RNA-Seq
Dairy sheep
Mammary gland transcriptome
Milk fat synthesis
Nutrigenomics
Marine lipids
description 9 páginas, 4 figuras.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/148292
url http://hdl.handle.net/10261/148292
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-54587-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-66035-R
http://dx.doi.org/10.1038/srep45905
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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