Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition

16 páginas.- 6 figuras.- referencias

Detalles Bibliográficos
Autores: Prieto-Dapena, Pilar, Carranco, Raúl, Almoguera, Concepción, Jordano, Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394105
Acceso en línea:http://hdl.handle.net/10261/394105
Access Level:acceso abierto
Palabra clave:Seed-to-seedling transition
Short-term memory
Chromatin accessibility
SWI/SNF remodeling
HSFA9
Nicotiana tabacum
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spelling Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transitionPrieto-Dapena, PilarCarranco, RaúlAlmoguera, ConcepciónJordano, JuanSeed-to-seedling transitionShort-term memoryChromatin accessibilitySWI/SNF remodelingHSFA9Nicotiana tabacum16 páginas.- 6 figuras.- referenciasHSFA9 (A9) is a seed-specific transcription factor that contributes to seed longevity in sunflower. A9 also links the regulation of seed maturation with that of early seedling greening through its effects on various light receptors. Transcriptomic analyses of transgenic tobacco seeds suggested that A9 might affect chromatin remodeling. Here, using formaldehyde-assisted isolation of regulatory elements (FAIRE) and micrococcal nuclease digestion, we analyzed the A9 effects on chromatin accessibility shortly after seed imbibition in the proximal promoter region of developmentally relevant genes, including HY5 and PHYA. A9, expressed from a seed-specific promoter, enhanced chromatin accessibility in the analyzed regions. Converse, promoter-specific, effects were observed upon loss-of-function of tobacco A9 (NtA9) in transgenic seeds. Furthermore, a memory effect was observed, as the induced chromatin accessibility persisted for up to 4 days after seed imbibition, when A9 was no longer detected. The A9-induced chromatin effects involved labile/unstable nucleosomes placed at proximal promoter locations where A9 induced substantial nucleosomal depletion. Specific inhibitors of BRAHMA-like ATPase subunits of SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin-remodeling complexes and histone deacetylase (HDAC) impaired A9-induced memory. Thus, SWI/SNF remodeling and HDAC activity mechanistically contribute to the A9-induced memory. Furthermore, SWI/SNF inhibition specifically reduced the HY5 and PHYA promoter accessibility in both transgenic and non-transgenic seeds. Our results identify HSFA9 as a potential master, short-term, ‘epigenetic’ regulator that operates in seeds in anticipation of seedling establishment. The new, A9-induced, somatic memory effect reported here may facilitate early seedling greening and stress tolerance during the seed-to-seedling developmental transition.This work wasfunded through project PID2020-112693RB-I00/AEI/10.13039/501100011033Peer reviewedJohn Wiley & SonsMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionPrieto-Dapena, Pilar [0000-0002-2826-8688]Carranco, Raúl [0000-0001-9359-9793]Almoguera, Concepción [0000-0002-7961-6507]Jordano, Juan [0000-0002-2583-4453]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/394105reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112693RB-I00http://dx.doi.org/10.1111/tpj.70261Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3941052026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
title Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
spellingShingle Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
Prieto-Dapena, Pilar
Seed-to-seedling transition
Short-term memory
Chromatin accessibility
SWI/SNF remodeling
HSFA9
Nicotiana tabacum
title_short Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
title_full Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
title_fullStr Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
title_full_unstemmed Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
title_sort Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition
dc.creator.none.fl_str_mv Prieto-Dapena, Pilar
Carranco, Raúl
Almoguera, Concepción
Jordano, Juan
author Prieto-Dapena, Pilar
author_facet Prieto-Dapena, Pilar
Carranco, Raúl
Almoguera, Concepción
Jordano, Juan
author_role author
author2 Carranco, Raúl
Almoguera, Concepción
Jordano, Juan
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Prieto-Dapena, Pilar [0000-0002-2826-8688]
Carranco, Raúl [0000-0001-9359-9793]
Almoguera, Concepción [0000-0002-7961-6507]
Jordano, Juan [0000-0002-2583-4453]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Seed-to-seedling transition
Short-term memory
Chromatin accessibility
SWI/SNF remodeling
HSFA9
Nicotiana tabacum
topic Seed-to-seedling transition
Short-term memory
Chromatin accessibility
SWI/SNF remodeling
HSFA9
Nicotiana tabacum
description 16 páginas.- 6 figuras.- referencias
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394105
url http://hdl.handle.net/10261/394105
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112693RB-I00
http://dx.doi.org/10.1111/tpj.70261
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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