Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro

Bone tissue regeneration requires the use of 3D scaffolds which mimic the architecture of the natural extracellular matrix, creating an adequate microenvironment for bone cell growth. Such 3D scaffolds need surface properties suitable for biological recognition in the early stage of cell adhesion, n...

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Autores: Cicuéndez Maroto, Mónica, Malmsten, Martin, Doadrio Villarejo, Juan Carlos, Portolés Pérez, María Teresa, Izquierdo Barba, Isabel, Vallet Regí, María Dulce Nombre
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/35381
Acceso en línea:https://hdl.handle.net/20.500.14352/35381
Access Level:acceso abierto
Palabra clave:546
615.46
Enhanced osteoblast adhesion
Cell -Adhesion
Mesoporous materials
Calcium Phosphates
Delivery-Systems
Bioactive glass
Bone
Surfaces
Design
Hydroxyapatite
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/35381
network_acronym_str ES
network_name_str España
repository_id_str
spelling Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macroCicuéndez Maroto, MónicaMalmsten, MartinDoadrio Villarejo, Juan CarlosPortolés Pérez, María TeresaIzquierdo Barba, IsabelVallet Regí, María Dulce Nombre546615.46Enhanced osteoblast adhesionCell -AdhesionMesoporous materialsCalcium PhosphatesDelivery-SystemsBioactive glassBoneSurfacesDesignHydroxyapatiteMaterialesQuímica inorgánica (Química)3312 Tecnología de Materiales2303 Química InorgánicaBone tissue regeneration requires the use of 3D scaffolds which mimic the architecture of the natural extracellular matrix, creating an adequate microenvironment for bone cell growth. Such 3D scaffolds need surface properties suitable for biological recognition in the early stage of cell adhesion, necessary to ensure complete cell colonization, retained cell functionality, and subsequently bone regeneration. Herein, hierarchical 3D scaffolds based on new hydroxyapatite/mesoporous glass nanocomposite bioceramic (MGHA) exhibiting different scales of porosity have been synthesized. These 3D scaffolds possess: (i) highly ordered mesopores with diameters of 10 nm; (ii) macropores with diameters in the 30-80 mu m range with interconnections of 1-10 mu m; and (iii) large macropores of ca. 500 mu m. To improve their surface properties, 3D scaffolds were modified through direct functionalization with amine propyl groups, which notably improve preosteoblast adhesion, proliferation (2.3 fold), differentiation (4.8 fold) and further cell colonization of these scaffolds. The observed enhancement can be related to these amine groups which favour early adhesion, e. g., based on nonspecific protein adsorption as was demonstrated by ellipsometry. These results suggest that the combination of hierarchical structure design and amine surface modification of hydroxyapatite/mesoporous nanocomposite scaffolds yields a double increase in cell proliferation, as well as a quadruple increase in cell differentiation, demonstrating the potential of these nanocomposite materials for bone tissue regeneration purposes.Royal Society of ChemistryUniversidad Complutense de Madrid20142014-01-0120142014-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/35381reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/353812026-06-02T12:44:21Z
dc.title.none.fl_str_mv Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
title Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
spellingShingle Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
Cicuéndez Maroto, Mónica
546
615.46
Enhanced osteoblast adhesion
Cell -Adhesion
Mesoporous materials
Calcium Phosphates
Delivery-Systems
Bioactive glass
Bone
Surfaces
Design
Hydroxyapatite
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
title_short Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
title_full Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
title_fullStr Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
title_full_unstemmed Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
title_sort Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro
dc.creator.none.fl_str_mv Cicuéndez Maroto, Mónica
Malmsten, Martin
Doadrio Villarejo, Juan Carlos
Portolés Pérez, María Teresa
Izquierdo Barba, Isabel
Vallet Regí, María Dulce Nombre
author Cicuéndez Maroto, Mónica
author_facet Cicuéndez Maroto, Mónica
Malmsten, Martin
Doadrio Villarejo, Juan Carlos
Portolés Pérez, María Teresa
Izquierdo Barba, Isabel
Vallet Regí, María Dulce Nombre
author_role author
author2 Malmsten, Martin
Doadrio Villarejo, Juan Carlos
Portolés Pérez, María Teresa
Izquierdo Barba, Isabel
Vallet Regí, María Dulce Nombre
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 546
615.46
Enhanced osteoblast adhesion
Cell -Adhesion
Mesoporous materials
Calcium Phosphates
Delivery-Systems
Bioactive glass
Bone
Surfaces
Design
Hydroxyapatite
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
topic 546
615.46
Enhanced osteoblast adhesion
Cell -Adhesion
Mesoporous materials
Calcium Phosphates
Delivery-Systems
Bioactive glass
Bone
Surfaces
Design
Hydroxyapatite
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
description Bone tissue regeneration requires the use of 3D scaffolds which mimic the architecture of the natural extracellular matrix, creating an adequate microenvironment for bone cell growth. Such 3D scaffolds need surface properties suitable for biological recognition in the early stage of cell adhesion, necessary to ensure complete cell colonization, retained cell functionality, and subsequently bone regeneration. Herein, hierarchical 3D scaffolds based on new hydroxyapatite/mesoporous glass nanocomposite bioceramic (MGHA) exhibiting different scales of porosity have been synthesized. These 3D scaffolds possess: (i) highly ordered mesopores with diameters of 10 nm; (ii) macropores with diameters in the 30-80 mu m range with interconnections of 1-10 mu m; and (iii) large macropores of ca. 500 mu m. To improve their surface properties, 3D scaffolds were modified through direct functionalization with amine propyl groups, which notably improve preosteoblast adhesion, proliferation (2.3 fold), differentiation (4.8 fold) and further cell colonization of these scaffolds. The observed enhancement can be related to these amine groups which favour early adhesion, e. g., based on nonspecific protein adsorption as was demonstrated by ellipsometry. These results suggest that the combination of hierarchical structure design and amine surface modification of hydroxyapatite/mesoporous nanocomposite scaffolds yields a double increase in cell proliferation, as well as a quadruple increase in cell differentiation, demonstrating the potential of these nanocomposite materials for bone tissue regeneration purposes.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/35381
url https://hdl.handle.net/20.500.14352/35381
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411092852113408
score 15,301629

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