Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry

The study of real samples is more complicated than the study of other systems. However, the inherent advantages of UV–vis absorption spectroelectrochemistry should overcome some difficulties related to direct measurements in complex matrices. For this reason, a singular spectroelectrochemistry devic...

Descripción completa

Detalles Bibliográficos
Autores: Garoz Ruiz, Jesús, Heras, Aránzazu, Colina, Álvaro
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Universidad de Burgos (UBU)
Repositorio:Repositorio Institucional de la Universidad de Burgos (RIUBU)
OAI Identifier:oai:riubu.ubu.es:10259/4947
Acceso en línea:http://hdl.handle.net/10259/4947
Access Level:acceso abierto
Palabra clave:Química analítica
Chemistry, Analytic
id ES_76ea55fdd56ef6f48b8938eb022d3501
oai_identifier_str oai:riubu.ubu.es:10259/4947
network_acronym_str ES
network_name_str España
repository_id_str
spelling Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistryGaroz Ruiz, JesúsHeras, AránzazuColina, ÁlvaroQuímica analíticaChemistry, AnalyticThe study of real samples is more complicated than the study of other systems. However, the inherent advantages of UV–vis absorption spectroelectrochemistry should overcome some difficulties related to direct measurements in complex matrices. For this reason, a singular spectroelectrochemistry device has been fabricated and validated. The novel cell is based on single-walled carbon nanotubes, which are filtered and subsequently press-transferred on a polyethylene terephthalate support using a stencil with a custom design. With this new methodology, working, counter, and reference electrodes are completely flat on the surface, where two optical fibers are fixed in a long optical path length configuration. To demonstrate the usefulness of this device and the power of spectroelectrochemistry techniques to solve problems of the current world, this device is used to quantitatively detect the concentration of ascorbic acid in a complex matrix such as a fruit, directly, without any previous sample pretreatment. The ease to fabricate the device, the advantages related to its use, and the excellent results obtained not only with univariate but also with multivariate analysis, shed more light on the analysis of samples as they occur in nature. According to the particular features of this cell, to the best of our knowledge this is the first spectroelectrochemical sensor that can be inserted directly in a biological matrix, laying the groundwork to perform in vivo measurements in a near future.Ministerio de Economıá y Competitividad (CTQ2014-55583-R, CTQ2014-61914-EXP, CTQ2015- 71955-REDT) and Junta de Castilla y León (BU033-U16)American Chemical Society201820182017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10259/4947reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)instname:Universidad de Burgos (UBU)InglésAnalytical Chemistry. 2017, V. 89, n. 3, p. 1815-1822https://doi.org/10.1021/acs.analchem.6b04155info:eu-repo/grantAgreement/MINECO/CTQ2014-55583-R/info:eu-repo/grantAgreement/MINECO/CTQ2014-61914-EXP/info:eu-repo/grantAgreement/MINECO/CTQ2015-71955-REDT/info:eu-repo/grantAgreement/JCyL/BU033-U16/info:eu-repo/semantics/openAccessoai:riubu.ubu.es:10259/49472026-05-28T07:56:11Z
dc.title.none.fl_str_mv Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
title Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
spellingShingle Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
Garoz Ruiz, Jesús
Química analítica
Chemistry, Analytic
title_short Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
title_full Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
title_fullStr Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
title_full_unstemmed Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
title_sort Direct determination of ascorbic acid in a grapefruit: paving the way for in vivo spectroelectrochemistry
dc.creator.none.fl_str_mv Garoz Ruiz, Jesús
Heras, Aránzazu
Colina, Álvaro
author Garoz Ruiz, Jesús
author_facet Garoz Ruiz, Jesús
Heras, Aránzazu
Colina, Álvaro
author_role author
author2 Heras, Aránzazu
Colina, Álvaro
author2_role author
author
dc.subject.none.fl_str_mv Química analítica
Chemistry, Analytic
topic Química analítica
Chemistry, Analytic
description The study of real samples is more complicated than the study of other systems. However, the inherent advantages of UV–vis absorption spectroelectrochemistry should overcome some difficulties related to direct measurements in complex matrices. For this reason, a singular spectroelectrochemistry device has been fabricated and validated. The novel cell is based on single-walled carbon nanotubes, which are filtered and subsequently press-transferred on a polyethylene terephthalate support using a stencil with a custom design. With this new methodology, working, counter, and reference electrodes are completely flat on the surface, where two optical fibers are fixed in a long optical path length configuration. To demonstrate the usefulness of this device and the power of spectroelectrochemistry techniques to solve problems of the current world, this device is used to quantitatively detect the concentration of ascorbic acid in a complex matrix such as a fruit, directly, without any previous sample pretreatment. The ease to fabricate the device, the advantages related to its use, and the excellent results obtained not only with univariate but also with multivariate analysis, shed more light on the analysis of samples as they occur in nature. According to the particular features of this cell, to the best of our knowledge this is the first spectroelectrochemical sensor that can be inserted directly in a biological matrix, laying the groundwork to perform in vivo measurements in a near future.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10259/4947
url http://hdl.handle.net/10259/4947
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Analytical Chemistry. 2017, V. 89, n. 3, p. 1815-1822
https://doi.org/10.1021/acs.analchem.6b04155
info:eu-repo/grantAgreement/MINECO/CTQ2014-55583-R/
info:eu-repo/grantAgreement/MINECO/CTQ2014-61914-EXP/
info:eu-repo/grantAgreement/MINECO/CTQ2015-71955-REDT/
info:eu-repo/grantAgreement/JCyL/BU033-U16/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)
instname:Universidad de Burgos (UBU)
instname_str Universidad de Burgos (UBU)
reponame_str Repositorio Institucional de la Universidad de Burgos (RIUBU)
collection Repositorio Institucional de la Universidad de Burgos (RIUBU)
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411084865110016
score 15,301629

Similares en LA Referencia