Characterization of microsatellite loci in Erysimum mediohispanicum (Brassicaceae) and cross-amplification in related species
• Premise of the study: We have developed and optimized microsatellite loci from a genomic library of Erysimum mediohispani- cum. Microsatellites were also tested for cross-amplification in 31 other Erysimum species. • Methods and Results: A total of 10 microsatellite loci were successfully amplifie...
| Authors: | , , , , , , |
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| Format: | article |
| Publication Date: | 2011 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/51635 |
| Online Access: | http://hdl.handle.net/10261/51635 |
| Access Level: | Open access |
| Keyword: | Brassicaceae cross-amplification Erysimum mediohispanicum microsatellite |
| Summary: | • Premise of the study: We have developed and optimized microsatellite loci from a genomic library of Erysimum mediohispani- cum. Microsatellites were also tested for cross-amplification in 31 other Erysimum species. • Methods and Results: A total of 10 microsatellite loci were successfully amplified. They were polymorphic for 81 E. mediohis- panicum individuals from two locations in Sierra Nevada (southeastern Spain), which showed similar patterns of genetic diver- sity. On average, microsatellites had 8.6 alleles per locus and an expected heterozygosity of 0.69. Only one locus significantly departed from Hardy–Weinberg equilibrium in both locations. Most of the markers successfully amplified in other Erysimum species. • Conclusions: The genetic attributes of microsatellite loci will allow their application to population genetic studies in Erysimum, such as genetic differentiation and structure, gene flow, pollinator-mediated speciation, and hybridization studies |
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