Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,

Novel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N’,...

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Autores: Voitechovič, E., Bratov, Andrey, Abramova, Natalia, Razumienė, J., Kirsanov, D., Legin, Andrey, Lakshmi, D., Piletsky, S., Whitcombe, M., Ivanova-Mitseva, P. K.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/164221
Acceso en línea:http://hdl.handle.net/10261/164221
Access Level:acceso abierto
Palabra clave:Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
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spelling Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,Voitechovič, E.Bratov, AndreyAbramova, NataliaRazumienė, J.Kirsanov, D.Legin, AndreyLakshmi, D.Piletsky, S.Whitcombe, M.Ivanova-Mitseva, P. K.Interdigitated electrode arrayDETPQQ dependent enzymePolyanilineBiosensorsNovel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N’,N’-diethyldithiocarbamoylethylamidoethyl) aniline (AnD) was deposited onto 3D-IDEA by chemical polymerisation. It was found that the polymer film resistance depends on the redox-potential of the solution. For the first time polyAnD was used as enzyme immobilisation matrix. Pyrroloquinolinequinone (PQQ) dependent alcohol and glucose dehydrogenases were immobilized on 3D-IDEA covered with polyAnD by two different methods. 3D-IDEA sensors with enzymes, which were immobilised by physisorption on polyAnD layer, showed specific response in the presence of 1 μM of the corresponding substrates. Obtained results revealed that PQQ dependent dehydrogenases can re-oxidize on polyAnD via direct electron transfer (DET) from enzyme active site to the polymer surface. This process can be monitored by methods of electrochemical impedance spectroscopy (EIS) and chronoamperometry. Presented study shows that EIS method gives a useful tool for research of re-oxidation process and interaction of electroactive enzymes with conducting materials giving information required to construct and develop analytical devices.N. Abramova and A. Bratov acknowledge financial support from Spanish Ministry of Science and Innovation (projects AGL2008-05578-C05-05/01; IPT-2011-1055-900000 and CTQ2011-29163-C03-02). E. Voitechovic would like to acknowledge the financial support from St. Petersburg State University PostDoc Grant #12.50.1191.2014 and PhD Student Research Traineeship from the Lithuanian Science Council.Peer reviewedElsevierMinisterio de Economía, Industria y Competitividad (España)Ministerio de Ciencia e Innovación (España)Saint Petersburg State UniversityResearch Council of LithuaniaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/164221reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-54553-C3-1-Rhttp://doi.org/10.1016/j.electacta.2015.05.011Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1642212026-05-22T06:33:51Z
dc.title.none.fl_str_mv Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
spellingShingle Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
Voitechovič, E.
Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
title_short Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_full Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_fullStr Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_full_unstemmed Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_sort Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
dc.creator.none.fl_str_mv Voitechovič, E.
Bratov, Andrey
Abramova, Natalia
Razumienė, J.
Kirsanov, D.
Legin, Andrey
Lakshmi, D.
Piletsky, S.
Whitcombe, M.
Ivanova-Mitseva, P. K.
author Voitechovič, E.
author_facet Voitechovič, E.
Bratov, Andrey
Abramova, Natalia
Razumienė, J.
Kirsanov, D.
Legin, Andrey
Lakshmi, D.
Piletsky, S.
Whitcombe, M.
Ivanova-Mitseva, P. K.
author_role author
author2 Bratov, Andrey
Abramova, Natalia
Razumienė, J.
Kirsanov, D.
Legin, Andrey
Lakshmi, D.
Piletsky, S.
Whitcombe, M.
Ivanova-Mitseva, P. K.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Saint Petersburg State University
Research Council of Lithuania
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
topic Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
description Novel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N’,N’-diethyldithiocarbamoylethylamidoethyl) aniline (AnD) was deposited onto 3D-IDEA by chemical polymerisation. It was found that the polymer film resistance depends on the redox-potential of the solution. For the first time polyAnD was used as enzyme immobilisation matrix. Pyrroloquinolinequinone (PQQ) dependent alcohol and glucose dehydrogenases were immobilized on 3D-IDEA covered with polyAnD by two different methods. 3D-IDEA sensors with enzymes, which were immobilised by physisorption on polyAnD layer, showed specific response in the presence of 1 μM of the corresponding substrates. Obtained results revealed that PQQ dependent dehydrogenases can re-oxidize on polyAnD via direct electron transfer (DET) from enzyme active site to the polymer surface. This process can be monitored by methods of electrochemical impedance spectroscopy (EIS) and chronoamperometry. Presented study shows that EIS method gives a useful tool for research of re-oxidation process and interaction of electroactive enzymes with conducting materials giving information required to construct and develop analytical devices.
publishDate 2015
dc.date.none.fl_str_mv 2015
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/164221
url http://hdl.handle.net/10261/164221
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-54553-C3-1-R
http://doi.org/10.1016/j.electacta.2015.05.011
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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