Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures

13 pags., 5 figs. -- This article belongs to the Special Issue Galectins: Their Network and Roles in Infection/Immunity/Tumor Growth Control 2021

Detalhes bibliográficos
Autores: Campanero-Rhodes, María Asunción, Kalograiaki, Ioanna, Euba, Begoña, Llobet, E., Ardá, Ana, Jiménez-Barbero, Jesús, Garmendia, Juncal, Solís, Dolores
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/253326
Acesso em linha:http://hdl.handle.net/10261/253326
Access Level:Acceso aberto
Palavra-chave:Bacteria microarrays
Galectins
Host–pathogen interactions
Klebsiella pneumoniae
Nontypeable Haemophilus influenzae
Lipopolysaccharides
Lipooligosaccharides
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spelling Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architecturesCampanero-Rhodes, María AsunciónKalograiaki, IoannaEuba, BegoñaLlobet, E.Ardá, AnaJiménez-Barbero, JesúsGarmendia, JuncalSolís, DoloresBacteria microarraysGalectinsHost–pathogen interactionsKlebsiella pneumoniaeNontypeable Haemophilus influenzaeLipopolysaccharidesLipooligosaccharides13 pags., 5 figs. -- This article belongs to the Special Issue Galectins: Their Network and Roles in Infection/Immunity/Tumor Growth Control 2021Galectins bind various pathogens through recognition of distinct carbohydrate structures. In this work, we examined the binding of four human galectins to the Gram-negative bacteria Klebsiella pneumoniae (Kpn) and non-typeable Haemophilus influenzae (NTHi), which display different surface glycans. In particular, Kpn cells are covered by a polysaccharide capsule and display an O-chain-containing lipopolysaccharide (LPS), whereas NTHi is not capsulated and its LPS, termed lipooligosacccharide (LOS), does not contain O-chain. Binding assays to microarray-printed bacteria revealed that galectins-3, -4, and -8, but not galectin-1, bind to Kpn and NTHi cells, and confocal microscopy attested binding to bacterial cells in suspension. The three galectins bound to array-printed Kpn LPS. Moreover, analysis of galectin binding to mutant Kpn cells evidenced that the O-chain is the docking point for galectins on wild type Kpn. Galectins-3, -4, and -8 also bound the NTHi LOS. Microarray-assisted comparison of the binding to full-length and truncated LOSs, as well as to wild type and mutant cells, supported LOS involvement in galectin binding to NTHi. However, deletion of the entire LOS oligosaccharide chain actually increased binding to NTHi cells, indicating the availability of other ligands on the bacterial surface, as similarly inferred for Kpn cells devoid of both O-chain and capsule. Altogether, the results illustrate galectins’ versatility for recognizing different bacterial structures, and point out the occurrence of so far overlooked galectin ligands on bacterial surfaces.This research was funded by the Spanish Ministry of Science, Innovation, and Universities, the Spanish State Research Agency, and the European Regional Development Fund (Grants RTI2018-099985-B-I00, RTI2018-096369-B-I00, and RTI2018-094751-B-C21, MCIU/AEI/FEDER, UE), and by the CIBER of Respiratory Diseases (CIBERES), an initiative from the Spanish Institute of Health Carlos III (ISCIII).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Instituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/253326reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099985-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096369-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094751-B-C21https://doi.org/10.3390/biom11040595Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2533262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
title Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
spellingShingle Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
Campanero-Rhodes, María Asunción
Bacteria microarrays
Galectins
Host–pathogen interactions
Klebsiella pneumoniae
Nontypeable Haemophilus influenzae
Lipopolysaccharides
Lipooligosaccharides
title_short Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
title_full Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
title_fullStr Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
title_full_unstemmed Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
title_sort Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures
dc.creator.none.fl_str_mv Campanero-Rhodes, María Asunción
Kalograiaki, Ioanna
Euba, Begoña
Llobet, E.
Ardá, Ana
Jiménez-Barbero, Jesús
Garmendia, Juncal
Solís, Dolores
author Campanero-Rhodes, María Asunción
author_facet Campanero-Rhodes, María Asunción
Kalograiaki, Ioanna
Euba, Begoña
Llobet, E.
Ardá, Ana
Jiménez-Barbero, Jesús
Garmendia, Juncal
Solís, Dolores
author_role author
author2 Kalograiaki, Ioanna
Euba, Begoña
Llobet, E.
Ardá, Ana
Jiménez-Barbero, Jesús
Garmendia, Juncal
Solís, Dolores
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacteria microarrays
Galectins
Host–pathogen interactions
Klebsiella pneumoniae
Nontypeable Haemophilus influenzae
Lipopolysaccharides
Lipooligosaccharides
topic Bacteria microarrays
Galectins
Host–pathogen interactions
Klebsiella pneumoniae
Nontypeable Haemophilus influenzae
Lipopolysaccharides
Lipooligosaccharides
description 13 pags., 5 figs. -- This article belongs to the Special Issue Galectins: Their Network and Roles in Infection/Immunity/Tumor Growth Control 2021
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/253326
url http://hdl.handle.net/10261/253326
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099985-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096369-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094751-B-C21
https://doi.org/10.3390/biom11040595
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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