Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections

Three molecular typing methods (pulsed-field electrophoresis, localization of the mecA gene, and probing the vicinity of mec) have been used for the characterization of 40 catheter-related isolates of coagulase-negative staphylococci (CNS) in 14 patients admitted to the same hospital. The 40 isolate...

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Autores: Domínguez Luzón, Ma. Ángeles (María Ángeles), Liñares Louzao, Josefina, Pulido, A., Pérez, J. L., De Lencastre, H.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1996
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/55276
Acceso en línea:https://hdl.handle.net/2445/55276
Access Level:acceso abierto
Palabra clave:Infeccions per estafilococs
Cateterisme intravascular
Staphylococcal infections
Intravenous catheterization
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spelling Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infectionsDomínguez Luzón, Ma. Ángeles (María Ángeles)Liñares Louzao, JosefinaPulido, A.Pérez, J. L.De Lencastre, H.Infeccions per estafilococsCateterisme intravascularStaphylococcal infectionsIntravenous catheterizationThree molecular typing methods (pulsed-field electrophoresis, localization of the mecA gene, and probing the vicinity of mec) have been used for the characterization of 40 catheter-related isolates of coagulase-negative staphylococci (CNS) in 14 patients admitted to the same hospital. The 40 isolates yielded 14 different SmaI banding patterns and corresponding unique localizations of mecA, each associated with a unique ClaI mecA polymorph. In 6 of the 14 patients the contaminated skin at the catheter entry site was the source of 4 local infections and 2 cases of bacteremia. A contaminated hub was the origin of 2 local infections and 4 cases of bacteremia in 6 more patients. The remaining 2 patients had positive cultures from both skin and catheter hub. In each bacteremic patient, the CNS recovered from catheter-related sites (tip, skin, and/or hub) and the CNS recovered from blood were identical, but each of these matching isolates was unique to the particular patient, indicating a low rate of cross-infection from patient to patient. Although classical methods for typing CNS (e.g., biotype and antibiotype) are readily available for most hospital laboratories, they have limitations concerning reproducibility and discriminatory power. Molecular epidemiologic techniques can provide powerful support to traditional techniques in determining the etiologic role of CNS in the disease processMary Ann Liebert, Inc.2014201419962014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/55276Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1089/mdr.1996.2.423Microbial Drug Resistance, 1996, vol. 2, num. 4, p. 423-429http://dx.doi.org/10.1089/mdr.1996.2.423(c) Mary Ann Liebert, Inc., 1996info:eu-repo/semantics/openAccessoai:recercat.cat:2445/552762026-05-29T05:05:01Z
dc.title.none.fl_str_mv Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
title Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
spellingShingle Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
Domínguez Luzón, Ma. Ángeles (María Ángeles)
Infeccions per estafilococs
Cateterisme intravascular
Staphylococcal infections
Intravenous catheterization
title_short Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
title_full Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
title_fullStr Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
title_full_unstemmed Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
title_sort Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections
dc.creator.none.fl_str_mv Domínguez Luzón, Ma. Ángeles (María Ángeles)
Liñares Louzao, Josefina
Pulido, A.
Pérez, J. L.
De Lencastre, H.
author Domínguez Luzón, Ma. Ángeles (María Ángeles)
author_facet Domínguez Luzón, Ma. Ángeles (María Ángeles)
Liñares Louzao, Josefina
Pulido, A.
Pérez, J. L.
De Lencastre, H.
author_role author
author2 Liñares Louzao, Josefina
Pulido, A.
Pérez, J. L.
De Lencastre, H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Infeccions per estafilococs
Cateterisme intravascular
Staphylococcal infections
Intravenous catheterization
topic Infeccions per estafilococs
Cateterisme intravascular
Staphylococcal infections
Intravenous catheterization
description Three molecular typing methods (pulsed-field electrophoresis, localization of the mecA gene, and probing the vicinity of mec) have been used for the characterization of 40 catheter-related isolates of coagulase-negative staphylococci (CNS) in 14 patients admitted to the same hospital. The 40 isolates yielded 14 different SmaI banding patterns and corresponding unique localizations of mecA, each associated with a unique ClaI mecA polymorph. In 6 of the 14 patients the contaminated skin at the catheter entry site was the source of 4 local infections and 2 cases of bacteremia. A contaminated hub was the origin of 2 local infections and 4 cases of bacteremia in 6 more patients. The remaining 2 patients had positive cultures from both skin and catheter hub. In each bacteremic patient, the CNS recovered from catheter-related sites (tip, skin, and/or hub) and the CNS recovered from blood were identical, but each of these matching isolates was unique to the particular patient, indicating a low rate of cross-infection from patient to patient. Although classical methods for typing CNS (e.g., biotype and antibiotype) are readily available for most hospital laboratories, they have limitations concerning reproducibility and discriminatory power. Molecular epidemiologic techniques can provide powerful support to traditional techniques in determining the etiologic role of CNS in the disease process
publishDate 1996
dc.date.none.fl_str_mv 1996
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/55276
url https://hdl.handle.net/2445/55276
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1089/mdr.1996.2.423
Microbial Drug Resistance, 1996, vol. 2, num. 4, p. 423-429
http://dx.doi.org/10.1089/mdr.1996.2.423
dc.rights.none.fl_str_mv (c) Mary Ann Liebert, Inc., 1996
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Mary Ann Liebert, Inc., 1996
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc.
publisher.none.fl_str_mv Mary Ann Liebert, Inc.
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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