Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors

22 Pág.

Detalles Bibliográficos
Autores: Navarrete-López, Paula, Maroto, María, Pericuesta Camacho, Eva, Fernández González, Raúl, Lombó, Marta, Ramos Ibeas, Priscila, Gutiérrez-Adán, Alfonso
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/334954
Acceso en línea:http://hdl.handle.net/10261/334954
https://api.elsevier.com/content/abstract/scopus_id/85165642578
Access Level:acceso abierto
Palabra clave:Molecular biology
Omics
Transcriptomics
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spelling Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factorsNavarrete-López, PaulaMaroto, MaríaPericuesta Camacho, EvaFernández González, RaúlLombó, MartaRamos Ibeas, PriscilaGutiérrez-Adán, AlfonsoMolecular biologyOmicsTranscriptomics22 Pág.Karyopherins mediate the movement between the nucleus and cytoplasm of specific proteins in diverse cellular processes. Through a loss-of-function approach, we here examine the role of Karyopherin Subunit Alpha 2 (Kpna2) in spermatogenesis. Knockout male mice exhibited reduced body size and sperm motility, increased sperm abnormalities, and led to the dysregulation of testis gene expression and ultimately to infertility. Impaired mRNA expression mainly affected clusters of genes expressed in spermatids and spermatocytes. Downregulated genes included a set of genes that participate in cell adhesion and extracellular matrix (ECM) organization. We detected both the enrichment of some transcription factors that bind to regions around transcription start sites of downregulated genes and the impaired transport of specific factors to the nucleus of spermatid cells. We propose that Kpna2 is essential in the seminiferous tubules for promoting the translocation of testis-specific transcription factors that control the expression of genes related to ECM organization.This research was supported by Grants RTI2018-093548-B-I00 and PID2021-122507OB-I00 funded by MCIN/AEI/10.13039/501100011033/and European Union “NextGenerationEU”/PRTR). P.N was supported by a pre-doctoral fellowship from the Spanish Ministry of Science and Innovation (MICINN) (PRE2019-088813), and M.L was supported by a Juan de la Cierva postdoctoral contract (FJC2019-040385-I) from the MICINN. We gratefully thank CESGA (Supercomputing Center of Galicia) for providing the necessary resources for the data processing.Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionNavarrete-López, Paula [0000-0002-7093-2191]Maroto, María [0000-0001-8738-5932]Pericuesta Camacho, Eva [0000-0001-9387-3270]Fernández González, Raúl [0000-0003-1989-2945]Lombó, Marta [0000-0002-2042-798X]Ramos Ibeas, Priscila [0000-0003-4578-0189]Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/334954https://api.elsevier.com/content/abstract/scopus_id/85165642578reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122507OB-I00info:eu-repo/grantAgreement/AEI//PRE2019-088813info:eu-repo/grantAgreement/AEI//FJC2019-040385-IDepartamento de Reproducción Animal​ https://doi.org/10.1016/j.isci.2023.107134Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3349542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
title Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
spellingShingle Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
Navarrete-López, Paula
Molecular biology
Omics
Transcriptomics
title_short Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
title_full Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
title_fullStr Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
title_full_unstemmed Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
title_sort Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
dc.creator.none.fl_str_mv Navarrete-López, Paula
Maroto, María
Pericuesta Camacho, Eva
Fernández González, Raúl
Lombó, Marta
Ramos Ibeas, Priscila
Gutiérrez-Adán, Alfonso
author Navarrete-López, Paula
author_facet Navarrete-López, Paula
Maroto, María
Pericuesta Camacho, Eva
Fernández González, Raúl
Lombó, Marta
Ramos Ibeas, Priscila
Gutiérrez-Adán, Alfonso
author_role author
author2 Maroto, María
Pericuesta Camacho, Eva
Fernández González, Raúl
Lombó, Marta
Ramos Ibeas, Priscila
Gutiérrez-Adán, Alfonso
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Navarrete-López, Paula [0000-0002-7093-2191]
Maroto, María [0000-0001-8738-5932]
Pericuesta Camacho, Eva [0000-0001-9387-3270]
Fernández González, Raúl [0000-0003-1989-2945]
Lombó, Marta [0000-0002-2042-798X]
Ramos Ibeas, Priscila [0000-0003-4578-0189]
Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Molecular biology
Omics
Transcriptomics
topic Molecular biology
Omics
Transcriptomics
description 22 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/334954
https://api.elsevier.com/content/abstract/scopus_id/85165642578
url http://hdl.handle.net/10261/334954
https://api.elsevier.com/content/abstract/scopus_id/85165642578
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122507OB-I00
info:eu-repo/grantAgreement/AEI//PRE2019-088813
info:eu-repo/grantAgreement/AEI//FJC2019-040385-I
Departamento de Reproducción Animal​ 
https://doi.org/10.1016/j.isci.2023.107134
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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