Plant-Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors
[EN] Potato virus X (PVX), a filamentous, positive-sense RNA plant virus, has been engineered into a molecular tool for diverse biotechnological applications, including cancer cell targeting. Here, we present the production and functional characterization of genetically-encoded PVX-derived nanoparti...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:dnet:riunet______::1c16e1e7ff8ab9156d114627d26957fe |
| Acceso en línea: | https://riunet.upv.es/handle/10251/233577 |
| Access Level: | acceso abierto |
| Palabra clave: | Epidermal growth factor receptor (EGFR) Human epidermal growth factor 2 (HER2) Nanobodies Plant virus Potato virus X (PVX) Viral nanoparticles |
| Sumario: | [EN] Potato virus X (PVX), a filamentous, positive-sense RNA plant virus, has been engineered into a molecular tool for diverse biotechnological applications, including cancer cell targeting. Here, we present the production and functional characterization of genetically-encoded PVX-derived nanoparticles decorated with nanobodies targeting two common receptors in human cancer cells, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2). We first generated a series of PVX-derived nanoparticles displaying distinct nanobodies against EGFR and HER2 in Nicotiana benthamiana plants. Self-assembly and structural integrity of the recombinant nanoparticles were confirmed by immunogold electron microscopy. We next characterized in vitro the cancer-cell binding capacity of the different recombinant viral nanoparticles (VNPs) by flow cytometry and confocal microscopy. Select VNPs were further assayed in a pilot in vivo study using tumor-bearing mice. Preliminary results showed that nanobody decoration can increase retention time and myeloid cell recruitment in the tumor microenvironment in HER2+ mouse tumor models in vivo. Nanobody-displaying PVX-derived nanoparticles may constitute a new plant-produced biotechnological product for cancer immunotherapy. |
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