Tetraspanin CD81 regulates HSV-1 infection

Different members of the tetraspanin superfamily have been described to regulate different virus infectious cycles at several stages: viral entry, viral replication or virion exit or infectivity. In addition, tetraspanin CD81 regulates HIV reverse transcription through its association with the dNTP...

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Autores: Benayas, Beátriz, Sastre, Isabel, López Martín, Soraya, Oo, Adrián, Kim, Baek, Bullido Gómez-Heras, María Jesús, Aldudo, Jesús, Yáñez Mo, María
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/728540
Acceso en línea:https://hdl.handle.net/10486/728540
https://dx.doi.org/10.1007/s00430-020-00684-0
Access Level:acceso abierto
Palabra clave:Tetraspanin
CD81
herpes simplex-1
SAMHD1
viral replication
Biología y Biomedicina / Biología
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spelling Tetraspanin CD81 regulates HSV-1 infectionBenayas, BeátrizSastre, IsabelLópez Martín, SorayaOo, AdriánKim, BaekBullido Gómez-Heras, María JesúsAldudo, JesúsYáñez Mo, MaríaTetraspaninCD81herpes simplex-1SAMHD1viral replicationBiología y Biomedicina / BiologíaDifferent members of the tetraspanin superfamily have been described to regulate different virus infectious cycles at several stages: viral entry, viral replication or virion exit or infectivity. In addition, tetraspanin CD81 regulates HIV reverse transcription through its association with the dNTP hydrolase SAMHD1. Here we aimed at analysing the role of CD81 in Herpes simplex virus 1 infectivity using a neuroblastoma cell model. For this purpose, we generated a CD81 KO cell line using the CRISPR/Cas9 technology. Despite being CD81 a plasma membrane protein, CD81 KO cells showed no defects in viral entry nor in the expression of early protein markers. In contrast, glycoprotein B and C, which require viral DNA replication for their expression, were significantly reduced in CD81 KO infected cells. Indeed, HSV-1 DNA replication and the formation of new infectious particles were severely compromised in CD81 KO cells. We could not detect significant changes in SAMHD1 total expression levels, but a relocalization into endosomal structures was observed in CD81 KO cells. In summary, CD81 KO cells showed impaired viral DNA replication and produced greatly diminished viral titersThis work has been supported by grants BIO2017-86500-R from Ministerio Español de Economía y Competitividad (MINECO) to MY-M and SAF2017-85747-R to MJB as well as USA NIH R01 AI136581 and AI150451 to BKSpringerFacultad de CienciasDepartamento de Biología MolecularMicrodominios de Membrana en Vesículas Extracelulares, Adhesión y Migración CelularAgencia Estatal de Investigación20202020-05-27research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10486/728540https://dx.doi.org/10.1007/s00430-020-00684-032500359reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7285402026-06-23T12:46:27Z
dc.title.none.fl_str_mv Tetraspanin CD81 regulates HSV-1 infection
title Tetraspanin CD81 regulates HSV-1 infection
spellingShingle Tetraspanin CD81 regulates HSV-1 infection
Benayas, Beátriz
Tetraspanin
CD81
herpes simplex-1
SAMHD1
viral replication
Biología y Biomedicina / Biología
title_short Tetraspanin CD81 regulates HSV-1 infection
title_full Tetraspanin CD81 regulates HSV-1 infection
title_fullStr Tetraspanin CD81 regulates HSV-1 infection
title_full_unstemmed Tetraspanin CD81 regulates HSV-1 infection
title_sort Tetraspanin CD81 regulates HSV-1 infection
dc.creator.none.fl_str_mv Benayas, Beátriz
Sastre, Isabel
López Martín, Soraya
Oo, Adrián
Kim, Baek
Bullido Gómez-Heras, María Jesús
Aldudo, Jesús
Yáñez Mo, María
author Benayas, Beátriz
author_facet Benayas, Beátriz
Sastre, Isabel
López Martín, Soraya
Oo, Adrián
Kim, Baek
Bullido Gómez-Heras, María Jesús
Aldudo, Jesús
Yáñez Mo, María
author_role author
author2 Sastre, Isabel
López Martín, Soraya
Oo, Adrián
Kim, Baek
Bullido Gómez-Heras, María Jesús
Aldudo, Jesús
Yáñez Mo, María
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias
Departamento de Biología Molecular
Microdominios de Membrana en Vesículas Extracelulares, Adhesión y Migración Celular
Agencia Estatal de Investigación
dc.subject.none.fl_str_mv Tetraspanin
CD81
herpes simplex-1
SAMHD1
viral replication
Biología y Biomedicina / Biología
topic Tetraspanin
CD81
herpes simplex-1
SAMHD1
viral replication
Biología y Biomedicina / Biología
description Different members of the tetraspanin superfamily have been described to regulate different virus infectious cycles at several stages: viral entry, viral replication or virion exit or infectivity. In addition, tetraspanin CD81 regulates HIV reverse transcription through its association with the dNTP hydrolase SAMHD1. Here we aimed at analysing the role of CD81 in Herpes simplex virus 1 infectivity using a neuroblastoma cell model. For this purpose, we generated a CD81 KO cell line using the CRISPR/Cas9 technology. Despite being CD81 a plasma membrane protein, CD81 KO cells showed no defects in viral entry nor in the expression of early protein markers. In contrast, glycoprotein B and C, which require viral DNA replication for their expression, were significantly reduced in CD81 KO infected cells. Indeed, HSV-1 DNA replication and the formation of new infectious particles were severely compromised in CD81 KO cells. We could not detect significant changes in SAMHD1 total expression levels, but a relocalization into endosomal structures was observed in CD81 KO cells. In summary, CD81 KO cells showed impaired viral DNA replication and produced greatly diminished viral titers
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-05-27
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10486/728540
https://dx.doi.org/10.1007/s00430-020-00684-0
32500359
url https://hdl.handle.net/10486/728540
https://dx.doi.org/10.1007/s00430-020-00684-0
identifier_str_mv 32500359
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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