Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro

16 p.-6 fig.-1 tab.

Detalhes bibliográficos
Autores: Makki, Mongia, Del Toro, Francisco, Necira, Khouloud, Tenllado, Francisco, Djilani-Khouadja, Fattouma, Canto, Tomás
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/262380
Acesso em linha:http://hdl.handle.net/10261/262380
Access Level:acceso abierto
Palavra-chave:HCPro and virus adaptation
Potyvirus virulence and HCPro
HCPro suppression of silencing
Potyvirus evolution
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spelling Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCProMakki, MongiaDel Toro, FranciscoNecira, KhouloudTenllado, FranciscoDjilani-Khouadja, FattoumaCanto, TomásHCPro and virus adaptationPotyvirus virulence and HCProHCPro suppression of silencingPotyvirus evolution16 p.-6 fig.-1 tab.The contribution of the HCPro factors expressed by several PVY isolates of different geographical origins (one from Scotland, one from Spain, and several from Tunisia) to differences in their virulence in Nicotiana benthamiana plants was investigated under two growing conditions: standard (st; 26 °C and current ambient levels of CO2), and climate change-associated (cc; 31 °C and elevated levels of CO2). In all cases, relative infection symptoms and viral titers were determined. The viral HCPro cistrons were also sequenced and amino-acid features of the encoded proteins were established, as well as phylogenetic distances. Additionally, the abilities of the HCPros of several isolates to suppress silencing were assessed under either growing condition. Overall, viral titers and infection symptoms decreased under cc vs. st conditions. However, within each growing condition, relative titers and symptoms were found to be isolate-specific, with titers and symptom severities not always correlating. Crucially, isolates expressing identical HCPros displayed different symptoms. In addition, all HCPro variants tested displayed comparable silencing suppression strengths. Therefore, HCPro alone could not be the main determinant of the relative differences in pathogenicity observed among the PVY isolates tested in this host, under the environments considered.This work was funded by a Research Grant from the Spanish Ministry of Innovation and Science (PID2019-109304RB-I00). MM and KN were both recipients of short-stay Fellowships at a Foreign Research Institution from the University of Tunis, El Manar.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)Université de Tunis - El ManarMakki, Mongia [0000-0002-3564-4549]Del Toro, Francisco [0000-0002-8635-7574]Tenllado, Francisco [0000-0002-5349-7642]Canto, Tomás [0000-0001-8017-6345]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/262380reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109304RB-I00https://doi.org/10.3390/plants10061086Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2623802026-05-22T06:33:51Z
dc.title.none.fl_str_mv Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
title Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
spellingShingle Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
Makki, Mongia
HCPro and virus adaptation
Potyvirus virulence and HCPro
HCPro suppression of silencing
Potyvirus evolution
title_short Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
title_full Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
title_fullStr Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
title_full_unstemmed Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
title_sort Differences in virulence among PVY isolates of different geographical origins when infecting an experimental host under two growing environments are not determined by HCPro
dc.creator.none.fl_str_mv Makki, Mongia
Del Toro, Francisco
Necira, Khouloud
Tenllado, Francisco
Djilani-Khouadja, Fattouma
Canto, Tomás
author Makki, Mongia
author_facet Makki, Mongia
Del Toro, Francisco
Necira, Khouloud
Tenllado, Francisco
Djilani-Khouadja, Fattouma
Canto, Tomás
author_role author
author2 Del Toro, Francisco
Necira, Khouloud
Tenllado, Francisco
Djilani-Khouadja, Fattouma
Canto, Tomás
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Université de Tunis - El Manar
Makki, Mongia [0000-0002-3564-4549]
Del Toro, Francisco [0000-0002-8635-7574]
Tenllado, Francisco [0000-0002-5349-7642]
Canto, Tomás [0000-0001-8017-6345]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv HCPro and virus adaptation
Potyvirus virulence and HCPro
HCPro suppression of silencing
Potyvirus evolution
topic HCPro and virus adaptation
Potyvirus virulence and HCPro
HCPro suppression of silencing
Potyvirus evolution
description 16 p.-6 fig.-1 tab.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/262380
url http://hdl.handle.net/10261/262380
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109304RB-I00
https://doi.org/10.3390/plants10061086
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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