In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development

Class IIa histone deacetylase (HDAC) subfamily members are tissue-specific gene repressors with crucial roles in development and differentiation processes. A prominent example is HDAC7, a class IIa HDAC that shows a lymphoid-specific expression pattern within the hematopoietic system. In this study,...

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Autores: Azagra Rodríguez, Alba, Román González, Lidia, Collazo, Olga, Rodríguez Ubreva, Javier, Yébenes, Virginia G. de, Barneda Zahonero, Bruna, Rodríguez Lumbiarres, Jairo, 1980-, Castro de Moura, Manuel, Grego Bessa, Joaquim, Fernández Duran, Irene, Islam, Abul B. M. M. K., Esteller, Manel, 1968-, Ramiro, Almudena R., Ballestar Tarín, Esteban, Parra Bola, Mª Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/111110
Acceso en línea:https://hdl.handle.net/2445/111110
Access Level:acceso abierto
Palabra clave:Cèl·lules B
Histones
Limfòcits
Gens
Proteïnes
B cells
Lymphocytes
Genes
Proteins
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spelling In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and developmentAzagra Rodríguez, AlbaRomán González, LidiaCollazo, OlgaRodríguez Ubreva, JavierYébenes, Virginia G. deBarneda Zahonero, BrunaRodríguez Lumbiarres, Jairo, 1980-Castro de Moura, ManuelGrego Bessa, JoaquimFernández Duran, IreneIslam, Abul B. M. M. K.Esteller, Manel, 1968-Ramiro, Almudena R.Ballestar Tarín, EstebanParra Bola, Mª IsabelCèl·lules BHistonesLimfòcitsGensProteïnesB cellsHistonesLymphocytesGenesProteinsClass IIa histone deacetylase (HDAC) subfamily members are tissue-specific gene repressors with crucial roles in development and differentiation processes. A prominent example is HDAC7, a class IIa HDAC that shows a lymphoid-specific expression pattern within the hematopoietic system. In this study, we explored its potential role in B cell development by generating a conditional knockout mouse model. Our study demonstrates for the first time that HDAC7 deletion dramatically blocks early B cell development and gives rise to a severe lymphopenia in peripheral organs, while also leading to pro-B cell lineage promiscuity. We find that HDAC7 represses myeloid and T lymphocyte genes in B cell progenitors through interaction with myocyte enhancer factor 2C (MEFC2). In B cell progenitors, HDAC7 is recruited to promoters and enhancers of target genes, and its absence leads to increased enrichment of histone active marks. Our results prove that HDAC7 is a bona fide transcriptional repressor essential for B cell development.Rockefeller University Press2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/111110Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1084/jem.20150821Journal of Experimental Medicine, 2016, vol. 213, num. 12, p. 2591-2601https://doi.org/10.1084/jem.20150821(c) Rockefeller University Press, 2016info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1111102026-05-27T06:46:51Z
dc.title.none.fl_str_mv In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
title In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
spellingShingle In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
Azagra Rodríguez, Alba
Cèl·lules B
Histones
Limfòcits
Gens
Proteïnes
B cells
Histones
Lymphocytes
Genes
Proteins
title_short In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
title_full In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
title_fullStr In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
title_full_unstemmed In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
title_sort In vivo conditional deletion of HDAC7 reveals its requirement to establish proper B lymphocyte identity and development
dc.creator.none.fl_str_mv Azagra Rodríguez, Alba
Román González, Lidia
Collazo, Olga
Rodríguez Ubreva, Javier
Yébenes, Virginia G. de
Barneda Zahonero, Bruna
Rodríguez Lumbiarres, Jairo, 1980-
Castro de Moura, Manuel
Grego Bessa, Joaquim
Fernández Duran, Irene
Islam, Abul B. M. M. K.
Esteller, Manel, 1968-
Ramiro, Almudena R.
Ballestar Tarín, Esteban
Parra Bola, Mª Isabel
author Azagra Rodríguez, Alba
author_facet Azagra Rodríguez, Alba
Román González, Lidia
Collazo, Olga
Rodríguez Ubreva, Javier
Yébenes, Virginia G. de
Barneda Zahonero, Bruna
Rodríguez Lumbiarres, Jairo, 1980-
Castro de Moura, Manuel
Grego Bessa, Joaquim
Fernández Duran, Irene
Islam, Abul B. M. M. K.
Esteller, Manel, 1968-
Ramiro, Almudena R.
Ballestar Tarín, Esteban
Parra Bola, Mª Isabel
author_role author
author2 Román González, Lidia
Collazo, Olga
Rodríguez Ubreva, Javier
Yébenes, Virginia G. de
Barneda Zahonero, Bruna
Rodríguez Lumbiarres, Jairo, 1980-
Castro de Moura, Manuel
Grego Bessa, Joaquim
Fernández Duran, Irene
Islam, Abul B. M. M. K.
Esteller, Manel, 1968-
Ramiro, Almudena R.
Ballestar Tarín, Esteban
Parra Bola, Mª Isabel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules B
Histones
Limfòcits
Gens
Proteïnes
B cells
Histones
Lymphocytes
Genes
Proteins
topic Cèl·lules B
Histones
Limfòcits
Gens
Proteïnes
B cells
Histones
Lymphocytes
Genes
Proteins
description Class IIa histone deacetylase (HDAC) subfamily members are tissue-specific gene repressors with crucial roles in development and differentiation processes. A prominent example is HDAC7, a class IIa HDAC that shows a lymphoid-specific expression pattern within the hematopoietic system. In this study, we explored its potential role in B cell development by generating a conditional knockout mouse model. Our study demonstrates for the first time that HDAC7 deletion dramatically blocks early B cell development and gives rise to a severe lymphopenia in peripheral organs, while also leading to pro-B cell lineage promiscuity. We find that HDAC7 represses myeloid and T lymphocyte genes in B cell progenitors through interaction with myocyte enhancer factor 2C (MEFC2). In B cell progenitors, HDAC7 is recruited to promoters and enhancers of target genes, and its absence leads to increased enrichment of histone active marks. Our results prove that HDAC7 is a bona fide transcriptional repressor essential for B cell development.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/111110
url https://hdl.handle.net/2445/111110
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1084/jem.20150821
Journal of Experimental Medicine, 2016, vol. 213, num. 12, p. 2591-2601
https://doi.org/10.1084/jem.20150821
dc.rights.none.fl_str_mv (c) Rockefeller University Press, 2016
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Rockefeller University Press, 2016
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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