Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes

Pancreatic islets are particularly vulnerable in the initial days after transplantation when multiple factors converge to damage the islet graft. The aim of this study was to investigate the expression profile of genes involved in damage and protection of beta-cells in the initial days after syngene...

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Autores: Rodríguez-Mulero, Silvia, Montanya Mias, Eduard
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/134788
Acceso en línea:https://hdl.handle.net/2445/134788
Access Level:acceso abierto
Palabra clave:Gens
Apoptosi
Expressió gènica
Illots de Langerhans
Metabolisme
Genes
Apoptosis
Gene expression
Islands of Langerhans
Metabolism
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spelling Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genesRodríguez-Mulero, SilviaMontanya Mias, EduardGensApoptosiExpressió gènicaIllots de LangerhansMetabolismeGenesApoptosisGene expressionIslands of LangerhansMetabolismPancreatic islets are particularly vulnerable in the initial days after transplantation when multiple factors converge to damage the islet graft. The aim of this study was to investigate the expression profile of genes involved in damage and protection of beta-cells in the initial days after syngeneic islet transplantation. We studied the expression of a set of selected genes involved in apoptosis (Bcl2, Bclx(L), Bax, Bad, Bid, and CHOP), cytokine defense, (SOCS-1 and SOCS-3), or free radical protection (Hmox1, Cu/Zn-SOD, Mn-SOD, and Hsp70). Because hyperglycemia has deleterious effects on islet transplantation outcome, we studied its effect on the expression of these genes. Five hundred islets were syngeneically transplanted under the kidney capsule of normoglycemic or streptozotocin-induced diabetic Lewis rats. Gene expression was analyzed by quantitative real-time RT-PCR in grafts 1, 3, and 7 days after transplantation, and in freshly isolated islets. The expression of proapoptotic genes Bid and CHOP, as well as protective genes Bclx(L), Socs1, Socs3, Hmox1, and MnSod, was maximally increased 1 day after transplantation, and in most cases it remained increased 7 days later, indicating the presence of a protective response against cell damage. In contrast, the expression of Bcl2, Bax, Bad, Cu/ZnSod, and Hsp70 genes did not change. Hyperglycemia did not modify the expression of most studied genes. However, MnSod and Ins2 expression was increased and reduced, respectively, on day 7 after transplantation to diabetic recipients, suggesting that hyperglycemia increased oxidative stress and deteriorated beta-cell function in transplanted islets.Cognizant Communication Corporation2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/134788Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3727/096368908786991524Cell Transplantation, 2008, vol. 17, num. 9, p. 1025-1034https://doi.org/10.3727/096368908786991524cc-by-nc (c) Cognizant Communication Corporation, 2008http://creativecommons.org/licenses/by-nc/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1347882026-05-27T06:46:51Z
dc.title.none.fl_str_mv Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
title Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
spellingShingle Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
Rodríguez-Mulero, Silvia
Gens
Apoptosi
Expressió gènica
Illots de Langerhans
Metabolisme
Genes
Apoptosis
Gene expression
Islands of Langerhans
Metabolism
title_short Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
title_full Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
title_fullStr Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
title_full_unstemmed Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
title_sort Islet graft response to transplantation injury includes up-regulation of protective as well as apoptotic genes
dc.creator.none.fl_str_mv Rodríguez-Mulero, Silvia
Montanya Mias, Eduard
author Rodríguez-Mulero, Silvia
author_facet Rodríguez-Mulero, Silvia
Montanya Mias, Eduard
author_role author
author2 Montanya Mias, Eduard
author2_role author
dc.subject.none.fl_str_mv Gens
Apoptosi
Expressió gènica
Illots de Langerhans
Metabolisme
Genes
Apoptosis
Gene expression
Islands of Langerhans
Metabolism
topic Gens
Apoptosi
Expressió gènica
Illots de Langerhans
Metabolisme
Genes
Apoptosis
Gene expression
Islands of Langerhans
Metabolism
description Pancreatic islets are particularly vulnerable in the initial days after transplantation when multiple factors converge to damage the islet graft. The aim of this study was to investigate the expression profile of genes involved in damage and protection of beta-cells in the initial days after syngeneic islet transplantation. We studied the expression of a set of selected genes involved in apoptosis (Bcl2, Bclx(L), Bax, Bad, Bid, and CHOP), cytokine defense, (SOCS-1 and SOCS-3), or free radical protection (Hmox1, Cu/Zn-SOD, Mn-SOD, and Hsp70). Because hyperglycemia has deleterious effects on islet transplantation outcome, we studied its effect on the expression of these genes. Five hundred islets were syngeneically transplanted under the kidney capsule of normoglycemic or streptozotocin-induced diabetic Lewis rats. Gene expression was analyzed by quantitative real-time RT-PCR in grafts 1, 3, and 7 days after transplantation, and in freshly isolated islets. The expression of proapoptotic genes Bid and CHOP, as well as protective genes Bclx(L), Socs1, Socs3, Hmox1, and MnSod, was maximally increased 1 day after transplantation, and in most cases it remained increased 7 days later, indicating the presence of a protective response against cell damage. In contrast, the expression of Bcl2, Bax, Bad, Cu/ZnSod, and Hsp70 genes did not change. Hyperglycemia did not modify the expression of most studied genes. However, MnSod and Ins2 expression was increased and reduced, respectively, on day 7 after transplantation to diabetic recipients, suggesting that hyperglycemia increased oxidative stress and deteriorated beta-cell function in transplanted islets.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/134788
url https://hdl.handle.net/2445/134788
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3727/096368908786991524
Cell Transplantation, 2008, vol. 17, num. 9, p. 1025-1034
https://doi.org/10.3727/096368908786991524
dc.rights.none.fl_str_mv cc-by-nc (c) Cognizant Communication Corporation, 2008
http://creativecommons.org/licenses/by-nc/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Cognizant Communication Corporation, 2008
http://creativecommons.org/licenses/by-nc/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cognizant Communication Corporation
publisher.none.fl_str_mv Cognizant Communication Corporation
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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