Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage

In this article bacterial carbonate mineralization treatments are proposed as a novel strategy for decayed fossils and palaeontological heritage conservation; specifically, by means of inoculation of Myxococcus xanthus, a bacterium of proven effectiveness in ornamental stone bioconsolidation. Biocon...

Descripción completa

Detalles Bibliográficos
Autores: Marín Ortega, Sílvia, Calvo Torras, Ma. de los Ángeles, Iglesias Campos, M. A. (Manuel Ángel)
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/218992
Acceso en línea:https://hdl.handle.net/2445/218992
Access Level:acceso abierto
Palabra clave:Paleontologia
Bioluminescència
Conservació i restauració
Paleontology
Bioluminescence
Conservation and restoration
id ES_757b8cc49bcfb33eeaa0fe59b95ff5f4
oai_identifier_str oai:recercat.cat:2445/218992
network_acronym_str ES
network_name_str España
repository_id_str
spelling Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritageMarín Ortega, SílviaCalvo Torras, Ma. de los ÁngelesIglesias Campos, M. A. (Manuel Ángel)PaleontologiaBioluminescènciaConservació i restauracióPaleontologyBioluminescenceConservation and restorationIn this article bacterial carbonate mineralization treatments are proposed as a novel strategy for decayed fossils and palaeontological heritage conservation; specifically, by means of inoculation of Myxococcus xanthus, a bacterium of proven effectiveness in ornamental stone bioconsolidation. Bioconsolidation treatments can be very effective, stable, nontoxic, environmentally friendly, and chemically compatible with fossil heritage. The method reproduces what nature has been doing for millennia with fossils that have been permineralized by bacterial calcium carbonate precipitation. There is, however, some concern that bacterial inoculation could lead to the growth of undesirable microbiota, which could subsequently damage the fossil substrate. Because of this, the use of bacteria on heritage items must be meticulously monitored and analysis strategies should be carried out to detect bacteria viability during and after treatments. For this purpose, adenosine triphosphate assay is proposed in this article as a fast, affordable, portable, and easy-to-use system for conservators. as ATP assay results are relative and difficult to relate to colony forming unit, this study aims to improve their applicability by examining the correlation between ATP analysis and total viable bacteria count in the specific case of M. xanthus. This research provides reference and correlatable data to obtain an approximate estimation of M. xanthus viable bacterial colonies based on relative light unit data.John Wiley & Sons2025202520222025info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion10 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/218992Articles publicats en revistes (Arts Conservació-Restauració)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1002/bio.4403Luminescence, 2022, vol. 37, num.12, p. 2129-2138https://doi.org/10.1002/bio.4403(c) John Wiley & Sons, 2022http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2189922026-05-29T05:05:01Z
dc.title.none.fl_str_mv Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
title Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
spellingShingle Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
Marín Ortega, Sílvia
Paleontologia
Bioluminescència
Conservació i restauració
Paleontology
Bioluminescence
Conservation and restoration
title_short Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
title_full Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
title_fullStr Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
title_full_unstemmed Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
title_sort Correlation tests between relative light unit and colony forming unit for improving adenosine triphosphate Bioluminescence analysis in bacterial consolidation treatments on palaeontological heritage
dc.creator.none.fl_str_mv Marín Ortega, Sílvia
Calvo Torras, Ma. de los Ángeles
Iglesias Campos, M. A. (Manuel Ángel)
author Marín Ortega, Sílvia
author_facet Marín Ortega, Sílvia
Calvo Torras, Ma. de los Ángeles
Iglesias Campos, M. A. (Manuel Ángel)
author_role author
author2 Calvo Torras, Ma. de los Ángeles
Iglesias Campos, M. A. (Manuel Ángel)
author2_role author
author
dc.subject.none.fl_str_mv Paleontologia
Bioluminescència
Conservació i restauració
Paleontology
Bioluminescence
Conservation and restoration
topic Paleontologia
Bioluminescència
Conservació i restauració
Paleontology
Bioluminescence
Conservation and restoration
description In this article bacterial carbonate mineralization treatments are proposed as a novel strategy for decayed fossils and palaeontological heritage conservation; specifically, by means of inoculation of Myxococcus xanthus, a bacterium of proven effectiveness in ornamental stone bioconsolidation. Bioconsolidation treatments can be very effective, stable, nontoxic, environmentally friendly, and chemically compatible with fossil heritage. The method reproduces what nature has been doing for millennia with fossils that have been permineralized by bacterial calcium carbonate precipitation. There is, however, some concern that bacterial inoculation could lead to the growth of undesirable microbiota, which could subsequently damage the fossil substrate. Because of this, the use of bacteria on heritage items must be meticulously monitored and analysis strategies should be carried out to detect bacteria viability during and after treatments. For this purpose, adenosine triphosphate assay is proposed in this article as a fast, affordable, portable, and easy-to-use system for conservators. as ATP assay results are relative and difficult to relate to colony forming unit, this study aims to improve their applicability by examining the correlation between ATP analysis and total viable bacteria count in the specific case of M. xanthus. This research provides reference and correlatable data to obtain an approximate estimation of M. xanthus viable bacterial colonies based on relative light unit data.
publishDate 2022
dc.date.none.fl_str_mv 2022
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/218992
url https://hdl.handle.net/2445/218992
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1002/bio.4403
Luminescence, 2022, vol. 37, num.12, p. 2129-2138
https://doi.org/10.1002/bio.4403
dc.rights.none.fl_str_mv (c) John Wiley & Sons, 2022
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) John Wiley & Sons, 2022
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Arts Conservació-Restauració)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410983575814144
score 15,811543

Similares en LA Referencia