Diarylethene-based Pt(II) molecular switches: a novel approch to photoactivated chemotherapy

[eng] The photoactivation of metal complexes with potential anticancer activity is a hot topic of current research, as it may lead to the development of more selective and efficient drugs. Photoactivated chemotherapy (PACT) with coordination compounds is usually based on photochemical processes taki...

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Detalles Bibliográficos
Autor: Presa i Rodríguez, Andreu
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/107757
Acceso en línea:https://hdl.handle.net/2445/107757
http://hdl.handle.net/10803/400875
Access Level:acceso abierto
Palabra clave:Medicaments antineoplàstics
Compostos organometàl·lics
Fotoquímica
Disseny de medicaments
Antineoplastic agents
Organometallic compounds
Drug design
Descripción
Sumario:[eng] The photoactivation of metal complexes with potential anticancer activity is a hot topic of current research, as it may lead to the development of more selective and efficient drugs. Photoactivated chemotherapy (PACT) with coordination compounds is usually based on photochemical processes taking place at the metal center. In contrast, an innovative ligand-mediated photoactivation approach that exploits the outstanding photochemical properties of diarylethenes has been developed in the present PhD thesis. Platinum(II) complexes from dithienylcyclopentene-based photoswitchable ligands have been designed and prepared. These systems exhibit two thermally stable, interconvertible photoisomeric forms, which present clearly distinct properties. The photochemical behavior of all ligands and metal complexes has been examined by means of 1H NMR and UV-Vis spectroscopies, and a number of crystal structures have been determined. Additionally, DFT calculations have been performed to analyze the effect produced by the attachment of different metal-binding units on the photochemical performance of the diarylethene synthon. Subsequently, the interaction of each photoisomer of the platinum(II) complexes with DNA has been thoroughly investigated using different techniques. These studies revealed that the light-triggered transformation of these systems effectively translates into different DNA binding modes and affinities, as desired for a photoactivatable drug. The antiproliferative activity of the complexes prepared has then been evaluated through various cell viability in vitro assays, which validated the potential applications of such diarylethene-based photoswitches for the development of a new class of photochemotherapeutic metallodrugs.