Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens

Malaria is a mosquito-borne disease caused by protozoans of the genus Plasmodium that affects both humans and wildlife. The fitness consequences of infections by avian malaria are well known in birds, however, little information exists on its impact on mosquitoes. Here we study how Culex pipiens mos...

Descripción completa

Detalles Bibliográficos
Autores: Garrigós, Marta, Ylla, Guillem, Martínez de la Puente, Josué, Figuerola, Jordi, Ruiz-López, María José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/399816
Acceso en línea:http://hdl.handle.net/10261/399816
https://api.elsevier.com/content/abstract/scopus_id/85179946343
Access Level:acceso abierto
Palabra clave:Plasmodium cathemerium
Plasmodium relictum
RNAseq
Avian malaria
Mosquito transcriptome
Vector‐borne parasites
id ES_74fbe4a741fb7cceda483a30e0cd7e7b
oai_identifier_str oai:digital.csic.es:10261/399816
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
title Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
spellingShingle Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
Garrigós, Marta
Plasmodium cathemerium
Plasmodium relictum
RNAseq
Avian malaria
Mosquito transcriptome
Vector‐borne parasites
title_short Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
title_full Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
title_fullStr Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
title_full_unstemmed Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
title_sort Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens
dc.creator.none.fl_str_mv Garrigós, Marta
Ylla, Guillem
Martínez de la Puente, Josué
Figuerola, Jordi
Ruiz-López, María José
author Garrigós, Marta
author_facet Garrigós, Marta
Ylla, Guillem
Martínez de la Puente, Josué
Figuerola, Jordi
Ruiz-López, María José
author_role author
author2 Ylla, Guillem
Martínez de la Puente, Josué
Figuerola, Jordi
Ruiz-López, María José
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Jagiellonian University in Kraków
Universidad de Granada
Garrigós, Marta [0000-0003-4825-1434]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Plasmodium cathemerium
Plasmodium relictum
RNAseq
Avian malaria
Mosquito transcriptome
Vector‐borne parasites
topic Plasmodium cathemerium
Plasmodium relictum
RNAseq
Avian malaria
Mosquito transcriptome
Vector‐borne parasites
description Malaria is a mosquito-borne disease caused by protozoans of the genus Plasmodium that affects both humans and wildlife. The fitness consequences of infections by avian malaria are well known in birds, however, little information exists on its impact on mosquitoes. Here we study how Culex pipiens mosquitoes transcriptionally respond to infection by two different Plasmodium species, P. relictum and P. cathemerium, differing in their virulence (mortality rate) and transmissibility (parasite presence in exposed mosquitoes' saliva). We studied the mosquito response to the infection at three critical stages of parasite development: the formation of ookinetes at 24 h post-infection (hpi), the release of sporozoites into the hemocoel at 10 days post-infection (dpi), and the storage of sporozoites in the salivary glands at 21 dpi. For each time point, we characterized the gene expression of mosquitoes infected with each P. relictum and P. cathemerium and mosquitoes fed on an uninfected bird and, subsequently, compared their transcriptomic responses. Differential gene expression analysis showed that most transcriptomic changes occurred during the early infection stage (24 hpi), especially when comparing P. relictum and P. cathemerium-infected mosquitoes. Differentially expressed genes in mosquitoes infected with each species were related mainly to the metabolism of the immune response, trypsin, and other serine-proteases. We conclude that these differences in response may partly play a role in the differential virulence and transmissibility previously observed between P. relictum and P. cathemerium in Cx. pipiens.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/399816
https://api.elsevier.com/content/abstract/scopus_id/85179946343
url http://hdl.handle.net/10261/399816
https://api.elsevier.com/content/abstract/scopus_id/85179946343
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/731060
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-095704-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118205GB-I00
Garrigós, Marta; Ylla, Guillem; Martínez de la Puente, Josué; Figuerola, Jordi; Ruiz-López, María José; 2023; Metadata for RNAseq project analysing differential expression in Culex pipiens mosquitoes infected by two avian Plasmodium species [Dataset]; DIGITAL.CSIC; https://doi.org/10.20350/digitalCSIC/15708
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1111/mec.17240
https://doi.org/10.1111/mec.17240
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410948088856576
spelling Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiensGarrigós, MartaYlla, GuillemMartínez de la Puente, JosuéFiguerola, JordiRuiz-López, María JoséPlasmodium cathemeriumPlasmodium relictumRNAseqAvian malariaMosquito transcriptomeVector‐borne parasitesMalaria is a mosquito-borne disease caused by protozoans of the genus Plasmodium that affects both humans and wildlife. The fitness consequences of infections by avian malaria are well known in birds, however, little information exists on its impact on mosquitoes. Here we study how Culex pipiens mosquitoes transcriptionally respond to infection by two different Plasmodium species, P. relictum and P. cathemerium, differing in their virulence (mortality rate) and transmissibility (parasite presence in exposed mosquitoes' saliva). We studied the mosquito response to the infection at three critical stages of parasite development: the formation of ookinetes at 24 h post-infection (hpi), the release of sporozoites into the hemocoel at 10 days post-infection (dpi), and the storage of sporozoites in the salivary glands at 21 dpi. For each time point, we characterized the gene expression of mosquitoes infected with each P. relictum and P. cathemerium and mosquitoes fed on an uninfected bird and, subsequently, compared their transcriptomic responses. Differential gene expression analysis showed that most transcriptomic changes occurred during the early infection stage (24 hpi), especially when comparing P. relictum and P. cathemerium-infected mosquitoes. Differentially expressed genes in mosquitoes infected with each species were related mainly to the metabolism of the immune response, trypsin, and other serine-proteases. We conclude that these differences in response may partly play a role in the differential virulence and transmissibility previously observed between P. relictum and P. cathemerium in Cx. pipiens.This publication was supported by the project Research Infrastructures for the control of vector-borne diseases (Infravec2; project number 6738), which has received funding from the European Union's Horizon 2020 research and innovation programme, under grant agreement No 731060; project PGC2018-095704-B-I00 from Agencia Española de Investigación supported by FEDER Funds from the European Union and the computing infrastructure provided by ICTS-RBD-CSIC. This study was also partially financed by the PID2020-118205GB-I00 grant to JMP funded by MCIN/AEI/10.13039/501100011033. MG was supported by a FPI grant (PRE2021-098544). GY contributions were supported by the Faculty of Biochemistry, Biophysics and Biotechnology at Jagiellonian University (Poland), under the Strategic Programme Excellence the Polo d'Innovazione di Genomica, Genetica e Biologia, Siena (Italy) for the samples Initiative. Funding for open access charge: Universidad de Granada/CBUA.Peer reviewedJohn Wiley & SonsEuropean CommissionAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Jagiellonian University in KrakówUniversidad de GranadaGarrigós, Marta [0000-0003-4825-1434]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/399816https://api.elsevier.com/content/abstract/scopus_id/85179946343reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/731060info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-095704-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118205GB-I00Garrigós, Marta; Ylla, Guillem; Martínez de la Puente, Josué; Figuerola, Jordi; Ruiz-López, María José; 2023; Metadata for RNAseq project analysing differential expression in Culex pipiens mosquitoes infected by two avian Plasmodium species [Dataset]; DIGITAL.CSIC; https://doi.org/10.20350/digitalCSIC/15708The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1111/mec.17240https://doi.org/10.1111/mec.17240Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3998162026-05-22T06:33:51Z
score 15,811543

Similares en LA Referencia