Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering
Bone tissue engineering has come on the scene to overcome the difficulties of the current treatment strategies. By combining biomaterials, active agents and growth factors, cells and nanomaterials, tissue engineering makes it possible to create new structures that enhance bone regeneration. Herein,...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad del País Vasco |
| Repositorio: | Addi. Archivo Digital para la Docencia y la Investigación |
| OAI Identifier: | oai:addi.ehu.eus:10810/57742 |
| Acceso en línea: | http://hdl.handle.net/10810/57742 |
| Access Level: | acceso abierto |
| Palabra clave: | 3D scaffold bone biomaterials nanoclay tissue engineering SDF-1 regeneration hydrogels BMP-2 |
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Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineeringErezuma Aldamiz-Etxebarria, ItsasneLukin Garmendia, IzeiaPimenta-Lopes, CarolinaVentura, FrancescGarcía García, PatriciaReyes, RicardoArnau, Mª RosaDelgado, AraceliTaebnia, NayereKadumudi, Firoz BabuDolatshahi-Pirouz, AlirezaOrive Arroyo, Gorka3D scaffoldbonebiomaterialsnanoclaytissue engineeringSDF-1regenerationhydrogelsBMP-2Bone tissue engineering has come on the scene to overcome the difficulties of the current treatment strategies. By combining biomaterials, active agents and growth factors, cells and nanomaterials, tissue engineering makes it possible to create new structures that enhance bone regeneration. Herein, hyaluronic acid and alginate were used to create biologically active hydrogels, and montmorillonite nanoclay was used to reinforce and stabilize them. The developed scaffolds were found to be biocompatible and osteogenic with mMSCs in vitro, especially those reinforced with the nanoclay, and allowed mineralization even in the absence of differentiation media. Moreover, an in vivo investigation was performed to establish the potential of the hydrogels to mend bone and act as cell-carriers and delivery platforms for SDF-1. Scaffolds embedded with SDF-1 exhibited the highest percentages of bone regeneration as well as of angiogenesis, which confirms the suitability of the scaffolds for bone. Although there are a number of obstacles to triumph over, these bioengineered structures showed potential as future bone regeneration treatments.This work was supported by the Spanish Ministry of Economy, Industry, and Competitiveness (PID2019-106094RB-I00/AEI/10.13039/501100011033) and technical assistance from the ICTS NANBIOSIS (Drug Formulation Unit, U10) at the University of the Basque Country. We also appreciate the support from the Basque Country Government (Grupos Consolidados, No ref: IT907-16) . I. Erezuma and I. Lukin thank to the Basque Government for the PhD grants (PRE_2021_2_0021 & PRE_2021_2_0023) . C. Pimenta-Lopes is a recipient of a F.P.U. fellowship from the Ministry of Universidades. A.D.-P. would like to acknowledge the Danish Council for Independent Research (Technology and Production Sciences, 8105-00003B) , and the VIDI research programme with project number R0004387, which is (partly) financed by The Netherlands Organisation for Scientific Research (NWO) . This work has also received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 951747.Elsevier202220222022info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/57742reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/PID2019-106094RB-I00/info:eu-repo/grantAgreement/EC/H2020/951747https://www.sciencedirect.com/science/article/pii/S0378517322004501?via%3Dihub#!info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Atribución 3.0 Españaoai:addi.ehu.eus:10810/577422026-06-18T09:23:17Z |
| dc.title.none.fl_str_mv |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering |
| title |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering |
| spellingShingle |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering Erezuma Aldamiz-Etxebarria, Itsasne 3D scaffold bone biomaterials nanoclay tissue engineering SDF-1 regeneration hydrogels BMP-2 |
| title_short |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering |
| title_full |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering |
| title_fullStr |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering |
| title_full_unstemmed |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering |
| title_sort |
Nanoclay-reinforced HA/alginate scaffolds as cell carriers and SDF-1 delivery-platforms for bone tissue engineering |
| dc.creator.none.fl_str_mv |
Erezuma Aldamiz-Etxebarria, Itsasne Lukin Garmendia, Izeia Pimenta-Lopes, Carolina Ventura, Francesc García García, Patricia Reyes, Ricardo Arnau, Mª Rosa Delgado, Araceli Taebnia, Nayere Kadumudi, Firoz Babu Dolatshahi-Pirouz, Alireza Orive Arroyo, Gorka |
| author |
Erezuma Aldamiz-Etxebarria, Itsasne |
| author_facet |
Erezuma Aldamiz-Etxebarria, Itsasne Lukin Garmendia, Izeia Pimenta-Lopes, Carolina Ventura, Francesc García García, Patricia Reyes, Ricardo Arnau, Mª Rosa Delgado, Araceli Taebnia, Nayere Kadumudi, Firoz Babu Dolatshahi-Pirouz, Alireza Orive Arroyo, Gorka |
| author_role |
author |
| author2 |
Lukin Garmendia, Izeia Pimenta-Lopes, Carolina Ventura, Francesc García García, Patricia Reyes, Ricardo Arnau, Mª Rosa Delgado, Araceli Taebnia, Nayere Kadumudi, Firoz Babu Dolatshahi-Pirouz, Alireza Orive Arroyo, Gorka |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
3D scaffold bone biomaterials nanoclay tissue engineering SDF-1 regeneration hydrogels BMP-2 |
| topic |
3D scaffold bone biomaterials nanoclay tissue engineering SDF-1 regeneration hydrogels BMP-2 |
| description |
Bone tissue engineering has come on the scene to overcome the difficulties of the current treatment strategies. By combining biomaterials, active agents and growth factors, cells and nanomaterials, tissue engineering makes it possible to create new structures that enhance bone regeneration. Herein, hyaluronic acid and alginate were used to create biologically active hydrogels, and montmorillonite nanoclay was used to reinforce and stabilize them. The developed scaffolds were found to be biocompatible and osteogenic with mMSCs in vitro, especially those reinforced with the nanoclay, and allowed mineralization even in the absence of differentiation media. Moreover, an in vivo investigation was performed to establish the potential of the hydrogels to mend bone and act as cell-carriers and delivery platforms for SDF-1. Scaffolds embedded with SDF-1 exhibited the highest percentages of bone regeneration as well as of angiogenesis, which confirms the suitability of the scaffolds for bone. Although there are a number of obstacles to triumph over, these bioengineered structures showed potential as future bone regeneration treatments. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10810/57742 |
| url |
http://hdl.handle.net/10810/57742 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
info:eu-repo/grantAgreement/MINECO/PID2019-106094RB-I00/ info:eu-repo/grantAgreement/EC/H2020/951747 https://www.sciencedirect.com/science/article/pii/S0378517322004501?via%3Dihub#! |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/3.0/es/ Atribución 3.0 España |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/3.0/es/ Atribución 3.0 España |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Addi. Archivo Digital para la Docencia y la Investigación instname:Universidad del País Vasco |
| instname_str |
Universidad del País Vasco |
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Addi. Archivo Digital para la Docencia y la Investigación |
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Addi. Archivo Digital para la Docencia y la Investigación |
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15,300724 |