TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations

[EN] TET2 is involved in a variety of hematopoietic malignancies, mainly in myeloid malignancies. Most mutations of TET2 have been identified in myeloid disorders, but some have also recently been described in mature lymphoid neoplasms. In contrast to the large amount of data about mutations of TET2...

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Detalhes bibliográficos
Autores: Hernández-Sánchez, María, Rodríguez-Vicente, Ana E., Kohlmann, Alexander, Benito Sánchez, Rocío, García, Juan Luis, Risueño, Alberto, Fermiñán, Encarna, Rivas Sanz, Javier de las, González, Marcos, Hernández Rivas, Jesús María
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/140697
Acesso em linha:http://hdl.handle.net/10366/140697
Access Level:acceso abierto
Palavra-chave:TET2
Chronic Lymphocytic Leukemia
Leukemia, Lymphoid
leucemia linfoide
id ES_74e59fdf09fd1cd3b873828b95e1b7be
oai_identifier_str oai:gredos.usal.es:10366/140697
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repository_id_str
spelling TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 VariationsHernández-Sánchez, MaríaRodríguez-Vicente, Ana E.Kohlmann, AlexanderBenito Sánchez, RocíoGarcía, Juan LuisRisueño, AlbertoFermiñán, EncarnaRivas Sanz, Javier de lasGonzález, MarcosHernández Rivas, Jesús MaríaTET2Chronic Lymphocytic LeukemiaLeukemia, Lymphoidleucemia linfoide[EN] TET2 is involved in a variety of hematopoietic malignancies, mainly in myeloid malignancies. Most mutations of TET2 have been identified in myeloid disorders, but some have also recently been described in mature lymphoid neoplasms. In contrast to the large amount of data about mutations of TET2, some data are available for gene expression. Moreover, the role of TET2 in chronic lymphocytic leukemia (CLL) is unknown. This study analyzes both TET2 expression and mutations in 48 CLL patients. TET2 expression was analyzed by exon arrays and quantitative real-time polymerase chain reaction (qRT-PCR). Next-generation sequencing (NGS) technology was applied to investigate the presence of TET2 variations. Overexpression of TET2 was observed in B-cell lymphocytes from CLL patients compared with healthy donors (P = 0.004). In addition, in CLL patients, an overexpression of TET2 was also observed in the clonal B cells compared with the nontumoral cells (P = 0.002). However, no novel mutations were observed. Therefore, overexpression of TET2 in CLL seems to be unrelated to the presence of genomic TET2 variations.202020202014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10366/140697reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1406972026-06-07T06:28:51Z
dc.title.none.fl_str_mv TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
title TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
spellingShingle TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
Hernández-Sánchez, María
TET2
Chronic Lymphocytic Leukemia
Leukemia, Lymphoid
leucemia linfoide
title_short TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
title_full TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
title_fullStr TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
title_full_unstemmed TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
title_sort TET2 Overexpression in Chronic Lymphocytic Leukemia Is Unrelated to the Presence of TET2 Variations
dc.creator.none.fl_str_mv Hernández-Sánchez, María
Rodríguez-Vicente, Ana E.
Kohlmann, Alexander
Benito Sánchez, Rocío
García, Juan Luis
Risueño, Alberto
Fermiñán, Encarna
Rivas Sanz, Javier de las
González, Marcos
Hernández Rivas, Jesús María
author Hernández-Sánchez, María
author_facet Hernández-Sánchez, María
Rodríguez-Vicente, Ana E.
Kohlmann, Alexander
Benito Sánchez, Rocío
García, Juan Luis
Risueño, Alberto
Fermiñán, Encarna
Rivas Sanz, Javier de las
González, Marcos
Hernández Rivas, Jesús María
author_role author
author2 Rodríguez-Vicente, Ana E.
Kohlmann, Alexander
Benito Sánchez, Rocío
García, Juan Luis
Risueño, Alberto
Fermiñán, Encarna
Rivas Sanz, Javier de las
González, Marcos
Hernández Rivas, Jesús María
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TET2
Chronic Lymphocytic Leukemia
Leukemia, Lymphoid
leucemia linfoide
topic TET2
Chronic Lymphocytic Leukemia
Leukemia, Lymphoid
leucemia linfoide
description [EN] TET2 is involved in a variety of hematopoietic malignancies, mainly in myeloid malignancies. Most mutations of TET2 have been identified in myeloid disorders, but some have also recently been described in mature lymphoid neoplasms. In contrast to the large amount of data about mutations of TET2, some data are available for gene expression. Moreover, the role of TET2 in chronic lymphocytic leukemia (CLL) is unknown. This study analyzes both TET2 expression and mutations in 48 CLL patients. TET2 expression was analyzed by exon arrays and quantitative real-time polymerase chain reaction (qRT-PCR). Next-generation sequencing (NGS) technology was applied to investigate the presence of TET2 variations. Overexpression of TET2 was observed in B-cell lymphocytes from CLL patients compared with healthy donors (P = 0.004). In addition, in CLL patients, an overexpression of TET2 was also observed in the clonal B cells compared with the nontumoral cells (P = 0.002). However, no novel mutations were observed. Therefore, overexpression of TET2 in CLL seems to be unrelated to the presence of genomic TET2 variations.
publishDate 2014
dc.date.none.fl_str_mv 2014
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/140697
url http://hdl.handle.net/10366/140697
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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