Erratum: Photoswitchable dynasore analogs to control endocytosis with light

The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molec...

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Detalles Bibliográficos
Autores: Camarero, N., Trapero, Ana, Pérez-Jiménez, A., Macia, E., Gomila-Juaneda, A., Martín-Quirós, A, Nevola, L., Llobet, A., Llebaria, Amadeu, Hernando, J., Giralt, Ernest, Gorostiza, P.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/222510
Acceso en línea:http://hdl.handle.net/10261/222510
Access Level:acceso abierto
Palabra clave:Cellular dynamic processes
Descripción
Sumario:The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.