Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates

Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the infectio...

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Autores: Valmaseda, Aida, Bassat Orellana, Quique, Aide, Pedro Carlos Paulino, Cisteró, Pau, Jiménez, Alfons, Casellas, Aina, Machevo, Sonia, Aguilar, Ruth, Sigaúque, Betuel, Chauhan, Virander Singh, Langer, Christine, Beeson, James G., Chitnis, Chetan E., Alonso, Pedro, Gaur, Deepak, Mayor Aparicio, Alfredo Gabriel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/114189
Acceso en línea:https://hdl.handle.net/2445/114189
Access Level:acceso abierto
Palabra clave:Plasmodium falciparum
Hematies
Erythrocytes
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spelling Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolatesValmaseda, AidaBassat Orellana, QuiqueAide, Pedro Carlos PaulinoCisteró, PauJiménez, AlfonsCasellas, AinaMachevo, SoniaAguilar, RuthSigaúque, BetuelChauhan, Virander SinghLanger, ChristineBeeson, James G.Chitnis, Chetan E.Alonso, PedroGaur, DeepakMayor Aparicio, Alfredo GabrielPlasmodium falciparumHematiesPlasmodium falciparumErythrocytesPlasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the infection. 11 IRG transcript levels were measured in P. falciparum isolates by RT-PCR, and IgG/IgM against invasion ligands by Luminex(R), in 50 Mozambican adults, 25 children with severe malaria (SM) and 25 with uncomplicated malaria (UM). IRG expression differences among groups and associations between IRG expression and clinical/immunologic parameters were assessed. IRG expression diversity was higher in parasites infecting children than adults (p = 0.022). eba140 and ptramp expression decreased with age (p = 0.003 and 0.007, respectively) whereas p41 expression increased (p = 0.022). pfrh5 reduction in expression was abrupt early in life. Parasite density decreased with increasing pfrh5 expression (p < 0.001) and, only in children, parasite density increased with p41 expression (p = 0.007), and decreased with eba175 (p = 0.013). Antibody responses and IRG expression were not associated. In conclusion, IRG expression is associated with age and parasite density, but not with specific antibody responses in the acute phase of infection. Our results confirm the importance of multi-antigen vaccines development to avoid parasite immune escape when tested in malaria-exposed individuals.Nature Publishing2017201720172017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9 p.application/pdfhttps://hdl.handle.net/2445/114189Articles publicats en revistes (ISGlobal)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1038/s41598-017-05025-5Scientific Reports, 2017, vol. 7, num. 1, p. 4717http://dx.doi.org/10.1038/s41598-017-05025-5cc by (c) Valmaseda, Aida et al., 2017http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1141892026-05-29T05:05:01Z
dc.title.none.fl_str_mv Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
spellingShingle Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
Valmaseda, Aida
Plasmodium falciparum
Hematies
Plasmodium falciparum
Erythrocytes
title_short Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_full Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_fullStr Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_full_unstemmed Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
title_sort Host age and expression of genes involved in red blood cell invasion in Plasmodium falciparum field isolates
dc.creator.none.fl_str_mv Valmaseda, Aida
Bassat Orellana, Quique
Aide, Pedro Carlos Paulino
Cisteró, Pau
Jiménez, Alfons
Casellas, Aina
Machevo, Sonia
Aguilar, Ruth
Sigaúque, Betuel
Chauhan, Virander Singh
Langer, Christine
Beeson, James G.
Chitnis, Chetan E.
Alonso, Pedro
Gaur, Deepak
Mayor Aparicio, Alfredo Gabriel
author Valmaseda, Aida
author_facet Valmaseda, Aida
Bassat Orellana, Quique
Aide, Pedro Carlos Paulino
Cisteró, Pau
Jiménez, Alfons
Casellas, Aina
Machevo, Sonia
Aguilar, Ruth
Sigaúque, Betuel
Chauhan, Virander Singh
Langer, Christine
Beeson, James G.
Chitnis, Chetan E.
Alonso, Pedro
Gaur, Deepak
Mayor Aparicio, Alfredo Gabriel
author_role author
author2 Bassat Orellana, Quique
Aide, Pedro Carlos Paulino
Cisteró, Pau
Jiménez, Alfons
Casellas, Aina
Machevo, Sonia
Aguilar, Ruth
Sigaúque, Betuel
Chauhan, Virander Singh
Langer, Christine
Beeson, James G.
Chitnis, Chetan E.
Alonso, Pedro
Gaur, Deepak
Mayor Aparicio, Alfredo Gabriel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Plasmodium falciparum
Hematies
Plasmodium falciparum
Erythrocytes
topic Plasmodium falciparum
Hematies
Plasmodium falciparum
Erythrocytes
description Plasmodium falciparum proteins involved in erythrocyte invasion are main targets of acquired immunity and important vaccine candidates. We hypothesized that anti-parasite immunity acquired upon exposure would limit invasion-related gene (IRG) expression and affect the clinical impact of the infection. 11 IRG transcript levels were measured in P. falciparum isolates by RT-PCR, and IgG/IgM against invasion ligands by Luminex(R), in 50 Mozambican adults, 25 children with severe malaria (SM) and 25 with uncomplicated malaria (UM). IRG expression differences among groups and associations between IRG expression and clinical/immunologic parameters were assessed. IRG expression diversity was higher in parasites infecting children than adults (p = 0.022). eba140 and ptramp expression decreased with age (p = 0.003 and 0.007, respectively) whereas p41 expression increased (p = 0.022). pfrh5 reduction in expression was abrupt early in life. Parasite density decreased with increasing pfrh5 expression (p < 0.001) and, only in children, parasite density increased with p41 expression (p = 0.007), and decreased with eba175 (p = 0.013). Antibody responses and IRG expression were not associated. In conclusion, IRG expression is associated with age and parasite density, but not with specific antibody responses in the acute phase of infection. Our results confirm the importance of multi-antigen vaccines development to avoid parasite immune escape when tested in malaria-exposed individuals.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/114189
url https://hdl.handle.net/2445/114189
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1038/s41598-017-05025-5
Scientific Reports, 2017, vol. 7, num. 1, p. 4717
http://dx.doi.org/10.1038/s41598-017-05025-5
dc.rights.none.fl_str_mv cc by (c) Valmaseda, Aida et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Valmaseda, Aida et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing
publisher.none.fl_str_mv Nature Publishing
dc.source.none.fl_str_mv Articles publicats en revistes (ISGlobal)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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