Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003

BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) have emerged as pathogens that can cause food-borne infections and severe and potentially fatal illnesses in humans, such as haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). In Spain, like in many other countries, STEC strains...

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Authors: Mora, Azucena, Blanco, Miguel, Blanco, Jesús E, Dahbi, Ghizlane, López, Cecilia, Justel, Paula, Alonso, María Pilar, Echeita, Aurora, Bernárdez, María Isabel, González, Enrique A, Blanco, Jorge
Format: article
Publication Date:2007
Country:España
Institution:Instituto de Salud Carlos III (ISCIII)
Repository:Repisalud
Language:English
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/7422
Online Access:http://hdl.handle.net/20.500.12105/7422
Access Level:Open access
Keyword:Adhesins, Bacterial
Animals
Cattle
Colony Count, Microbial
Escherichia coli
Escherichia coli O157
Escherichia coli Proteins
Food Microbiology
Meat
Serotyping
Shiga Toxin
Spain
Virulence Factors
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oai_identifier_str oai:repisalud.isciii.es:20.500.12105/7422
network_acronym_str ES
network_name_str España
repository_id_str
spelling Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003Mora, AzucenaBlanco, MiguelBlanco, Jesús EDahbi, GhizlaneLópez, CeciliaJustel, PaulaAlonso, María PilarEcheita, AuroraBernárdez, María IsabelGonzález, Enrique ABlanco, JorgeAdhesins, BacterialAnimalsCattleColony Count, MicrobialEscherichia coliEscherichia coli O157Escherichia coli ProteinsFood MicrobiologyMeatSerotypingShiga ToxinSpainVirulence FactorsBACKGROUND: Shiga toxin-producing Escherichia coli (STEC) have emerged as pathogens that can cause food-borne infections and severe and potentially fatal illnesses in humans, such as haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). In Spain, like in many other countries, STEC strains have been frequently isolated from ruminants, and represent a significant cause of sporadic cases of human infection. In view of the lack of data on STEC isolated from food in Spain, the objectives of this study were to determine the level of microbiological contamination and the prevalence of STEC O157:H7 and non-O157 in a large sampling of minced beef collected from 30 local stores in Lugo city between 1995 and 2003. Also to establish if those STEC isolated from food possessed the same virulence profiles as STEC strains causing human infections. RESULTS: STEC were detected in 95 (12%) of the 785 minced beef samples tested. STEC O157:H7 was isolated from eight (1.0%) samples and non-O157 STEC from 90 (11%) samples. Ninety-six STEC isolates were further characterized by PCR and serotyping. PCR showed that 28 (29%) isolates carried stx1 genes, 49 (51%) possessed stx2 genes, and 19 (20%) both stx1 and stx2. Enterohemolysin (ehxA) and intimin (eae) virulence genes were detected in 43 (45%) and in 25 (26%) of the isolates, respectively. Typing of the eae variants detected four types: gamma1 (nine isolates), beta1 (eight isolates), epsilon1 (three isolates), and theta (two isolates). The majority (68%) of STEC isolates belonged to serotypes previously detected in human STEC and 38% to serotypes associated with STEC isolated from patients with HUS. Ten new serotypes not previously described in raw beef products were also detected. The highly virulent seropathotypes O26:H11 stx1 eae-beta1, O157:H7 stx1stx2 eae-gamma1 and O157:H7 stx2eae-gamma1, which are the most frequently observed among STEC causing human infections in Spain, were detected in 10 of the 96 STEC isolates. Furthermore, phage typing of STEC O157:H7 isolates showed that the majority (seven of eight isolates) belonged to the main phage types previously detected in STEC O157:H7 strains associated with severe human illnesses. CONCLUSION: The results of this study do not differ greatly from those reported in other countries with regard to prevalence of O157 and non-O157 STEC in minced beef. As we suspected, serotypes different from O157:H7 also play an important role in food contamination in Spain, including the highly virulent seropathotype O26:H11 stx1 eae-beta1. Thus, our data confirm minced beef in the city of Lugo as vehicles of highly pathogenic STEC. This requires that control measures to be introduced and implemented to increase the safety of minced beef.BioMed Central (BMC)Instituto de Salud Carlos IIIXunta de Galicia (España)Agencia Española de Cooperación Internacional para el DesarrolloUnión Europea. Comisión Europea20192019-04-0220072007-03-0120072007-03-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/7422reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES G03-025-COLIRED-O157 Not availableES PI052023 Not availableEC CT98-4093 Not availableEC CT98-3935 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/74222026-06-12T12:43:37Z
dc.title.none.fl_str_mv Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
title Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
spellingShingle Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
Mora, Azucena
Adhesins, Bacterial
Animals
Cattle
Colony Count, Microbial
Escherichia coli
Escherichia coli O157
Escherichia coli Proteins
Food Microbiology
Meat
Serotyping
Shiga Toxin
Spain
Virulence Factors
title_short Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
title_full Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
title_fullStr Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
title_full_unstemmed Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
title_sort Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
dc.creator.none.fl_str_mv Mora, Azucena
Blanco, Miguel
Blanco, Jesús E
Dahbi, Ghizlane
López, Cecilia
Justel, Paula
Alonso, María Pilar
Echeita, Aurora
Bernárdez, María Isabel
González, Enrique A
Blanco, Jorge
author Mora, Azucena
author_facet Mora, Azucena
Blanco, Miguel
Blanco, Jesús E
Dahbi, Ghizlane
López, Cecilia
Justel, Paula
Alonso, María Pilar
Echeita, Aurora
Bernárdez, María Isabel
González, Enrique A
Blanco, Jorge
author_role author
author2 Blanco, Miguel
Blanco, Jesús E
Dahbi, Ghizlane
López, Cecilia
Justel, Paula
Alonso, María Pilar
Echeita, Aurora
Bernárdez, María Isabel
González, Enrique A
Blanco, Jorge
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Xunta de Galicia (España)
Agencia Española de Cooperación Internacional para el Desarrollo
Unión Europea. Comisión Europea
dc.subject.none.fl_str_mv Adhesins, Bacterial
Animals
Cattle
Colony Count, Microbial
Escherichia coli
Escherichia coli O157
Escherichia coli Proteins
Food Microbiology
Meat
Serotyping
Shiga Toxin
Spain
Virulence Factors
topic Adhesins, Bacterial
Animals
Cattle
Colony Count, Microbial
Escherichia coli
Escherichia coli O157
Escherichia coli Proteins
Food Microbiology
Meat
Serotyping
Shiga Toxin
Spain
Virulence Factors
description BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) have emerged as pathogens that can cause food-borne infections and severe and potentially fatal illnesses in humans, such as haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). In Spain, like in many other countries, STEC strains have been frequently isolated from ruminants, and represent a significant cause of sporadic cases of human infection. In view of the lack of data on STEC isolated from food in Spain, the objectives of this study were to determine the level of microbiological contamination and the prevalence of STEC O157:H7 and non-O157 in a large sampling of minced beef collected from 30 local stores in Lugo city between 1995 and 2003. Also to establish if those STEC isolated from food possessed the same virulence profiles as STEC strains causing human infections. RESULTS: STEC were detected in 95 (12%) of the 785 minced beef samples tested. STEC O157:H7 was isolated from eight (1.0%) samples and non-O157 STEC from 90 (11%) samples. Ninety-six STEC isolates were further characterized by PCR and serotyping. PCR showed that 28 (29%) isolates carried stx1 genes, 49 (51%) possessed stx2 genes, and 19 (20%) both stx1 and stx2. Enterohemolysin (ehxA) and intimin (eae) virulence genes were detected in 43 (45%) and in 25 (26%) of the isolates, respectively. Typing of the eae variants detected four types: gamma1 (nine isolates), beta1 (eight isolates), epsilon1 (three isolates), and theta (two isolates). The majority (68%) of STEC isolates belonged to serotypes previously detected in human STEC and 38% to serotypes associated with STEC isolated from patients with HUS. Ten new serotypes not previously described in raw beef products were also detected. The highly virulent seropathotypes O26:H11 stx1 eae-beta1, O157:H7 stx1stx2 eae-gamma1 and O157:H7 stx2eae-gamma1, which are the most frequently observed among STEC causing human infections in Spain, were detected in 10 of the 96 STEC isolates. Furthermore, phage typing of STEC O157:H7 isolates showed that the majority (seven of eight isolates) belonged to the main phage types previously detected in STEC O157:H7 strains associated with severe human illnesses. CONCLUSION: The results of this study do not differ greatly from those reported in other countries with regard to prevalence of O157 and non-O157 STEC in minced beef. As we suspected, serotypes different from O157:H7 also play an important role in food contamination in Spain, including the highly virulent seropathotype O26:H11 stx1 eae-beta1. Thus, our data confirm minced beef in the city of Lugo as vehicles of highly pathogenic STEC. This requires that control measures to be introduced and implemented to increase the safety of minced beef.
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-03-01
2007
2007-03-01
2019
2019-04-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/7422
url http://hdl.handle.net/20.500.12105/7422
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES G03-025-COLIRED-O157 Not available
ES PI052023 Not available
EC CT98-4093 Not available
EC CT98-3935 Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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