Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms

15 páginas, 7 figuras

Bibliographic Details
Authors: Chacón, Johelen, Chaves-Araya, Stephanie, Mena, Gianni, Chang-Castillo, Arturo, Díaz, Cecilia, Bonilla, Fabián, Sasa, Mahmood, Calvete, Juan J., Sanz, Libia, Gutiérrez, José María, Lomonte, Bruno, Fernández, Julián
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/392118
Online Access:http://hdl.handle.net/10261/392118
Access Level:Open access
Keyword:Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
id ES_73d341120fda59c5864951dcbef19d2f
oai_identifier_str oai:digital.csic.es:10261/392118
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
title Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
spellingShingle Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
Chacón, Johelen
Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
title_short Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
title_full Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
title_fullStr Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
title_full_unstemmed Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
title_sort Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II Venoms
dc.creator.none.fl_str_mv Chacón, Johelen
Chaves-Araya, Stephanie
Mena, Gianni
Chang-Castillo, Arturo
Díaz, Cecilia
Bonilla, Fabián
Sasa, Mahmood
Calvete, Juan J.
Sanz, Libia
Gutiérrez, José María
Lomonte, Bruno
Fernández, Julián
author Chacón, Johelen
author_facet Chacón, Johelen
Chaves-Araya, Stephanie
Mena, Gianni
Chang-Castillo, Arturo
Díaz, Cecilia
Bonilla, Fabián
Sasa, Mahmood
Calvete, Juan J.
Sanz, Libia
Gutiérrez, José María
Lomonte, Bruno
Fernández, Julián
author_role author
author2 Chaves-Araya, Stephanie
Mena, Gianni
Chang-Castillo, Arturo
Díaz, Cecilia
Bonilla, Fabián
Sasa, Mahmood
Calvete, Juan J.
Sanz, Libia
Gutiérrez, José María
Lomonte, Bruno
Fernández, Julián
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Universidad de Costa Rica
Ministerio de Ciencia, Innovación y Universidades (España)
Calvete, Juan J. [0000-0001-5026-3122]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
topic Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
description 15 páginas, 7 figuras
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/392118
url http://hdl.handle.net/10261/392118
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119593GB-I00
Chacón, Johelen; Chaves-Araya, Stephanie; Mena, Gianni; Chang-Castillo, Arturo; Díaz, Cecilia; Bonilla, Fabián; Sasa, Mahmood; Calvete, Juan J.; Sanz, Libia; Gutierrez, José María; Lomonte, Bruno; Fernández, Julián. (2025). Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3: Supplementary Table S1: Peptides and proteins matched in B. nubestris venom in the B. nubestris and B. nigroviridis databases Supplementary Table S2: Peptides gathered by bottom-up peptidomics and venomics of reduced and carbamidomethylated B. nubestris SDS-PAGE bands of venom fractions, and their match in the transcriptomic databases of B. nubestris and B. nigroviridis. Supplementary Table S3: Relative quantification of B. nubestris venom peptidome and proteome. ACS Publications [Dataset]; https://doi.org/10.1021/acs.jproteome.4c01041.s003
https://doi.org/10.1021/acs.jproteome.4c01041
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410837737766912
spelling Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type-I and Type-II VenomsChacón, JohelenChaves-Araya, StephanieMena, GianniChang-Castillo, ArturoDíaz, CeciliaBonilla, FabiánSasa, MahmoodCalvete, Juan J.Sanz, LibiaGutiérrez, José MaríaLomonte, BrunoFernández, JuliánBothriechis nubestrisICP antivenomSnake venomicsTalamancan palm-pitviperMiRNAType-II dichotomyVenom type-I15 páginas, 7 figurasWe report the biochemical and proteomic characterization of the venom of the arboreal Talamancan palm-pitviper, Bothriechis nubestris, a species endemic to north and central parts of the Costa Rican Cordillera de Talamanca, at 2100 to 3000 m above sea level. The Talamancan palm-pitviper venom arsenal comprised the translated products of 26 unique transcripts into 10 toxin classes, where metalloproteinases PIIa and PIII represent the dominant components. In vitro, the venom proteolyzed azocasein and gelatin but showed no phospholipase A2 or human plasma coagulant activities. In vivo, B. nubestris venom exhibited an intravenous median lethal dose (LD50) of 21.5 (95% confidence interval: 15.6-29.5) μg/mouse, and a minimum hemorrhagic dose (MHD) of 1.85 μg. PoliVal-ICP antivenom neutralized the venom's lethal activity with a potency of 9.7 mg of venom/g of antivenom and significantly reduced the hemorrhagic effect. Comparison of venom gland transcriptomes and venom proteomes of B. nubestris and its closest congeneric relative, B. nigroviridis, revealed that highly conserved venom gland transcriptomes are differentially processed by each species to produce divergent (type-I vs type-II, respectively) venoms. This phenomenon contributes to the remarkable venom phenotypic variability found across the palm-pitviper phylogeny. A possible mechanism for the occurrence of type-I/type-II venom phenotypic dichotomy is discussed.This work was partly financed by grant BFU2020_PID2020-119593GB-I00 from the Ministerio de Ciencia e Innovación,Madrid (Spain), and Vicerrectoría de Investigación, Universidadde Costa Rica (project C0061). A sabbatical stay (from April 1stto September 30, 2019) of JJC at Instituto Clodomiro Picadowas financed by the Programa “Salvador de Madariaga” deEstancias de Movilidad de Profesores e Investigadores enCentros Extranjeros from the Ministerio de Ciencia, Innovación,y Universidades (ref PRX18/00592)Peer reviewedAmerican Chemical SocietyMinisterio de Ciencia e Innovación (España)Universidad de Costa RicaMinisterio de Ciencia, Innovación y Universidades (España)Calvete, Juan J. [0000-0001-5026-3122]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/392118reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119593GB-I00Chacón, Johelen; Chaves-Araya, Stephanie; Mena, Gianni; Chang-Castillo, Arturo; Díaz, Cecilia; Bonilla, Fabián; Sasa, Mahmood; Calvete, Juan J.; Sanz, Libia; Gutierrez, José María; Lomonte, Bruno; Fernández, Julián. (2025). Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3: Supplementary Table S1: Peptides and proteins matched in B. nubestris venom in the B. nubestris and B. nigroviridis databases Supplementary Table S2: Peptides gathered by bottom-up peptidomics and venomics of reduced and carbamidomethylated B. nubestris SDS-PAGE bands of venom fractions, and their match in the transcriptomic databases of B. nubestris and B. nigroviridis. Supplementary Table S3: Relative quantification of B. nubestris venom peptidome and proteome. ACS Publications [Dataset]; https://doi.org/10.1021/acs.jproteome.4c01041.s003https://doi.org/10.1021/acs.jproteome.4c01041Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3921182026-05-22T06:33:51Z
score 15.812429

Similar Items