Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi

The increasing limitation of agrochemicals for disease control is a major challenge for European agriculture and a spur to developing environmentally friendly approaches such as biological control. Entomopathogenic fungi, which have been used in the control of insect pests for a long time, also have...

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Autores: Miranda-Fuentes, Pedro, García-Carneros, Ana B., Montilla‐Carmona, Ana María, Molinero-Ruiz, Leire
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/227599
Acesso em linha:http://hdl.handle.net/10261/227599
Access Level:acceso abierto
Palavra-chave:Biological control
Entomopathogens
Integrated pest management
Plant pathogens
Soil-borne pathogens
Sunflower diseases
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spelling Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungiMiranda-Fuentes, PedroGarcía-Carneros, Ana B.Montilla‐Carmona, Ana MaríaMolinero-Ruiz, LeireBiological controlEntomopathogensIntegrated pest managementPlant pathogensSoil-borne pathogensSunflower diseasesThe increasing limitation of agrochemicals for disease control is a major challenge for European agriculture and a spur to developing environmentally friendly approaches such as biological control. Entomopathogenic fungi, which have been used in the control of insect pests for a long time, also have other uses, such as being antagonists of fungi, including plant pathogens. We determined the in vitro effect of three strains of Metarhizium brunneum and two of Beauveria bassiana against Verticillium dahliae and Cadophora helianthi, causal agents of sunflower wilts. Both M. brunneum and B. bassiana were able to inhibit the mycelial growth of the sunflower pathogens and, according to the dual culture and microscopy results, two types of antagonism were observed as being dependent on the strain: competition and/or antibiosis. Greenhouse experiments showed that, after soil treatments with entomopathogens and plant inoculation by root immersion in conidial suspensions of V. dahliae, the entomopathogens were able to efficiently persist in the soil, and three of the four strains even significantly reduced the severity of symptoms in sunflowers. Interestingly, molecular analysis showed that all the strains were able to establish themselves as endophytes in sunflowers in the absence of V. dahliae. When the plants were inoculated with V. dahliae, we detected the pathogen, but not the entomopathogen, in the sunflowers by molecular methods. The results of this work suggest that the protection conferred by M. brunneum and B. bassiana against verticillium wilt might not be plant‐located, but is probably the consequence of their competition with V. dahliae in the soil.This research was partially supported by the Spanish Ministry of Economy and Competitiveness project AGL2016‐80483‐R to Professor E. Quesada‐Moraga and the Spanish National Research Council project PIE201940E058 to L.M.R., and grant FPU16/03983 from the Ministry of Education, Culture and Sports (Spain) to P.M.F.John Wiley & SonsMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Ministerio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/227599reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016‐80483‐Rhttp://doi.org/10.1111/ppa.13230Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2275992026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
title Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
spellingShingle Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
Miranda-Fuentes, Pedro
Biological control
Entomopathogens
Integrated pest management
Plant pathogens
Soil-borne pathogens
Sunflower diseases
title_short Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
title_full Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
title_fullStr Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
title_full_unstemmed Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
title_sort Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi
dc.creator.none.fl_str_mv Miranda-Fuentes, Pedro
García-Carneros, Ana B.
Montilla‐Carmona, Ana María
Molinero-Ruiz, Leire
author Miranda-Fuentes, Pedro
author_facet Miranda-Fuentes, Pedro
García-Carneros, Ana B.
Montilla‐Carmona, Ana María
Molinero-Ruiz, Leire
author_role author
author2 García-Carneros, Ana B.
Montilla‐Carmona, Ana María
Molinero-Ruiz, Leire
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biological control
Entomopathogens
Integrated pest management
Plant pathogens
Soil-borne pathogens
Sunflower diseases
topic Biological control
Entomopathogens
Integrated pest management
Plant pathogens
Soil-borne pathogens
Sunflower diseases
description The increasing limitation of agrochemicals for disease control is a major challenge for European agriculture and a spur to developing environmentally friendly approaches such as biological control. Entomopathogenic fungi, which have been used in the control of insect pests for a long time, also have other uses, such as being antagonists of fungi, including plant pathogens. We determined the in vitro effect of three strains of Metarhizium brunneum and two of Beauveria bassiana against Verticillium dahliae and Cadophora helianthi, causal agents of sunflower wilts. Both M. brunneum and B. bassiana were able to inhibit the mycelial growth of the sunflower pathogens and, according to the dual culture and microscopy results, two types of antagonism were observed as being dependent on the strain: competition and/or antibiosis. Greenhouse experiments showed that, after soil treatments with entomopathogens and plant inoculation by root immersion in conidial suspensions of V. dahliae, the entomopathogens were able to efficiently persist in the soil, and three of the four strains even significantly reduced the severity of symptoms in sunflowers. Interestingly, molecular analysis showed that all the strains were able to establish themselves as endophytes in sunflowers in the absence of V. dahliae. When the plants were inoculated with V. dahliae, we detected the pathogen, but not the entomopathogen, in the sunflowers by molecular methods. The results of this work suggest that the protection conferred by M. brunneum and B. bassiana against verticillium wilt might not be plant‐located, but is probably the consequence of their competition with V. dahliae in the soil.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/227599
url http://hdl.handle.net/10261/227599
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016‐80483‐R
http://doi.org/10.1111/ppa.13230
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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