A multi-omic analysis reveals the regulatory role of CD180 during the response of macrophages to "Borrelia burgdorferi"

[EN]Macrophages are cells of the innate immune system with the ability to phagocytose and induce a global pattern of responses that depend on several signaling pathways. We have determined the biosignature of murine bone marrow-derived macrophages and human blood monocytes using transcriptomic and p...

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Detalles Bibliográficos
Autores: Carreras González, Ana, Navasa Mayo, Nicolás, Martín Ruiz, Itziar, Lavín, José Luis, Azkargorta, Mikel, Atondo, Estíbaliz, Barriales, Diego, Macías Cámara, Nuria, Pascual Itoiz, Miguel Ángel, Sampedro, Leticia, Tomás Cortázar, Julen, Peña Cearra, Ainize, Pellón, Aize, Prados Rosales, Rafael, Abecia, Leticia, Elortza, Félix, Aransay, Ana M., Rodriguez, Héctor, Anguita, Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24456
Acceso en línea:https://www.tandfonline.com/doi/full/10.1038/s41426-017-0018-5
https://hdl.handle.net/10612/24456
Access Level:acceso abierto
Palabra clave:Biología
Macrophages
Fagocitosis
Borrelia burgdorferi
Macrófagos
2412 Inmunología
2414 Microbiología
2403 Bioquímica
Descripción
Sumario:[EN]Macrophages are cells of the innate immune system with the ability to phagocytose and induce a global pattern of responses that depend on several signaling pathways. We have determined the biosignature of murine bone marrow-derived macrophages and human blood monocytes using transcriptomic and proteomic approaches. We identified a common pattern of genes that are transcriptionally regulated and overall indicate that the response to B. burgdorferi involves the interaction of spirochetal antigens with several inflammatory pathways corresponding to primary (triggered by pattern-recognition receptors) and secondary (induced by proinflammatory cytokines) responses. We also show that the Toll-like receptor family member CD180 is downregulated by the stimulation of macrophages, but not monocytes, with the spirochete. Silencing Cd180 results in increased phagocytosis while tempering the production of the proinflammatory cytokine TNF. Cd180-silenced cells produce increased levels of Itgam and surface CD11b, suggesting that the regulation of CD180 by the spirochete initiates a cascade that increases CR3-mediated phagocytosis of the bacterium while repressing the consequent inflammatory response.

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