Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers

During embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in...

Descripción completa

Detalles Bibliográficos
Autores: Ojalvo-Sanz, Ana Cristina, López-Mascaraque, Laura
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/273167
Acceso en línea:http://hdl.handle.net/10261/273167
Access Level:acceso abierto
Palabra clave:StarTrack
glial cell types
lineage
progenitor
Astrocytes
Oligodendroglia
NG2-glia
clonal analysis
cortex
Mice
id ES_73a2d84fb1cf6690e65ef8656c7a8c67
oai_identifier_str oai:digital.csic.es:10261/273167
network_acronym_str ES
network_name_str España
repository_id_str
spelling Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical LayersOjalvo-Sanz, Ana CristinaLópez-Mascaraque, LauraStarTrackglial cell typeslineageprogenitorAstrocytesOligodendrogliaNG2-gliaclonal analysiscortexMiceDuring embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in the cerebral cortex. However, the potential of individual progenitors to form glial lineages remains poorly understood. To further investigate the cell progeny of single pallial GFAP-expressing progenitors, we used the in vivo genetic lineage-tracing method, the UbC-(GFAP-PB)-StarTrack. After targeting those progenitors in embryonic mice brains, we tracked their adult glial progeny in lower cortical layers. Clonal analyses revealed the presence of clones containing sibling cells of either a glial cell type (uniform clones) or two different glial cell types (mixed clones). Further, the clonal size and rostro-caudal cell dispersion of sibling cells differed depending on the cell type. We concluded that pallial E14 neural progenitors are a heterogeneous cell population with respect to which glial cell type they produce, as well as the clonal size of their cell progeny.This research was supported by the Spanish Research Grants: PID2019-105218RB-I00 funded by MCIN/AEI/10.13039/501100011033 and CIVP9A5928 funded by Fundación Ramón Areces.Multidisciplinary Digital Publishing InstituteAgencia Estatal de Investigación (España)Fundación Ramón ArecesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/273167reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105218RB-I00http://dx.doi.org/10.3390/cells10113237Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2731672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
spellingShingle Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
Ojalvo-Sanz, Ana Cristina
StarTrack
glial cell types
lineage
progenitor
Astrocytes
Oligodendroglia
NG2-glia
clonal analysis
cortex
Mice
title_short Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_full Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_fullStr Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_full_unstemmed Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_sort Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
dc.creator.none.fl_str_mv Ojalvo-Sanz, Ana Cristina
López-Mascaraque, Laura
author Ojalvo-Sanz, Ana Cristina
author_facet Ojalvo-Sanz, Ana Cristina
López-Mascaraque, Laura
author_role author
author2 López-Mascaraque, Laura
author2_role author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Fundación Ramón Areces
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv StarTrack
glial cell types
lineage
progenitor
Astrocytes
Oligodendroglia
NG2-glia
clonal analysis
cortex
Mice
topic StarTrack
glial cell types
lineage
progenitor
Astrocytes
Oligodendroglia
NG2-glia
clonal analysis
cortex
Mice
description During embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in the cerebral cortex. However, the potential of individual progenitors to form glial lineages remains poorly understood. To further investigate the cell progeny of single pallial GFAP-expressing progenitors, we used the in vivo genetic lineage-tracing method, the UbC-(GFAP-PB)-StarTrack. After targeting those progenitors in embryonic mice brains, we tracked their adult glial progeny in lower cortical layers. Clonal analyses revealed the presence of clones containing sibling cells of either a glial cell type (uniform clones) or two different glial cell types (mixed clones). Further, the clonal size and rostro-caudal cell dispersion of sibling cells differed depending on the cell type. We concluded that pallial E14 neural progenitors are a heterogeneous cell population with respect to which glial cell type they produce, as well as the clonal size of their cell progeny.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/273167
url http://hdl.handle.net/10261/273167
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105218RB-I00
http://dx.doi.org/10.3390/cells10113237
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410824446017536
score 15,811543

Similares en LA Referencia