Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode

The development of neural tissue starts with the activation of early neural genes such as the SoxB1 transcription factors, which are expressed in response to signaling molecules. Neural progenitors in the inner ear are only generated in the anterior placodal domain, but the mechanisms that determine...

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Authors: Abelló Sumpsi, Gina, 1980-, Khatri, Safia, Radosevic, Marija, 1978-, Scotting, Paul, Giráldez, Fernando, Alsina i Español, Berta
Format: article
Status:Published version
Publication Date:2010
Country:España
Institution:Universitat Pompeu Fabra
Repository:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/23740
Online Access:http://hdl.handle.net/10230/23740
http://dx.doi.org/10.1016/j.ydbio.2009.12.027
Access Level:Open access
Keyword:Laberint (Orella) -- Anatomia
Pollastres -- Embrions -- Anatomia
Otic placode
SoxB1
FGF
BMP
AP patterning
Neural fate
Ectodermal signaling
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spelling Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placodeAbelló Sumpsi, Gina, 1980-Khatri, SafiaRadosevic, Marija, 1978-Scotting, PaulGiráldez, FernandoAlsina i Español, BertaLaberint (Orella) -- AnatomiaPollastres -- Embrions -- AnatomiaOtic placodeSoxB1FGFBMPAP patterningNeural fateEctodermal signalingThe development of neural tissue starts with the activation of early neural genes such as the SoxB1 transcription factors, which are expressed in response to signaling molecules. Neural progenitors in the inner ear are only generated in the anterior placodal domain, but the mechanisms that determine when and how otic neural fate is acquired are still unknown. Here, we show that Sox3 expression becomes restricted to the anterior territory of the chick otic field and that misexpression of Sox3 induces Sox2 and Delta1 in the non-neurogenic otic territory. This suggests that Sox3 plays a central role in the establishment of an otic neural fate. Furthermore, Sox3 down-regulates the expression of Lmx1b, a marker of the posterior non-neurogenic otic epithelium. The expression of Sox3 is maintained by the positive action of FGF8 derived from the otic ectoderm. On the contrary, BMP signaling does not have a major influence on neural commitment but instead regulates Lmx1b expression in the otic placode. Together, the data support the notion that Sox3 is critical for the development of the neural elements of the inner ear, and they highlight the importance of localized signaling from the ectoderm in establishing the neurogenic vs. non-neurogenic anteroposterior asymmetry that characterizes the early otic placode.This work was supported by grant BMC2005-2008 and MAE fellowship to SK and FPI fellowship to MR.Elsevier201520152010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/23740http://dx.doi.org/10.1016/j.ydbio.2009.12.027reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésDevelopmental Biology. 2010;339(1):166-78© Elsevier This is the published version of an article http://dx.doi.org/10.1016/j.ydbio.2009.12.027 that appeared in the journal Developmental Biology. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseinfo:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/237402026-06-12T07:21:37Z
dc.title.none.fl_str_mv Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
title Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
spellingShingle Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
Abelló Sumpsi, Gina, 1980-
Laberint (Orella) -- Anatomia
Pollastres -- Embrions -- Anatomia
Otic placode
SoxB1
FGF
BMP
AP patterning
Neural fate
Ectodermal signaling
title_short Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
title_full Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
title_fullStr Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
title_full_unstemmed Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
title_sort Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
dc.creator.none.fl_str_mv Abelló Sumpsi, Gina, 1980-
Khatri, Safia
Radosevic, Marija, 1978-
Scotting, Paul
Giráldez, Fernando
Alsina i Español, Berta
author Abelló Sumpsi, Gina, 1980-
author_facet Abelló Sumpsi, Gina, 1980-
Khatri, Safia
Radosevic, Marija, 1978-
Scotting, Paul
Giráldez, Fernando
Alsina i Español, Berta
author_role author
author2 Khatri, Safia
Radosevic, Marija, 1978-
Scotting, Paul
Giráldez, Fernando
Alsina i Español, Berta
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Laberint (Orella) -- Anatomia
Pollastres -- Embrions -- Anatomia
Otic placode
SoxB1
FGF
BMP
AP patterning
Neural fate
Ectodermal signaling
topic Laberint (Orella) -- Anatomia
Pollastres -- Embrions -- Anatomia
Otic placode
SoxB1
FGF
BMP
AP patterning
Neural fate
Ectodermal signaling
description The development of neural tissue starts with the activation of early neural genes such as the SoxB1 transcription factors, which are expressed in response to signaling molecules. Neural progenitors in the inner ear are only generated in the anterior placodal domain, but the mechanisms that determine when and how otic neural fate is acquired are still unknown. Here, we show that Sox3 expression becomes restricted to the anterior territory of the chick otic field and that misexpression of Sox3 induces Sox2 and Delta1 in the non-neurogenic otic territory. This suggests that Sox3 plays a central role in the establishment of an otic neural fate. Furthermore, Sox3 down-regulates the expression of Lmx1b, a marker of the posterior non-neurogenic otic epithelium. The expression of Sox3 is maintained by the positive action of FGF8 derived from the otic ectoderm. On the contrary, BMP signaling does not have a major influence on neural commitment but instead regulates Lmx1b expression in the otic placode. Together, the data support the notion that Sox3 is critical for the development of the neural elements of the inner ear, and they highlight the importance of localized signaling from the ectoderm in establishing the neurogenic vs. non-neurogenic anteroposterior asymmetry that characterizes the early otic placode.
publishDate 2010
dc.date.none.fl_str_mv 2010
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/23740
http://dx.doi.org/10.1016/j.ydbio.2009.12.027
url http://hdl.handle.net/10230/23740
http://dx.doi.org/10.1016/j.ydbio.2009.12.027
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Developmental Biology. 2010;339(1):166-78
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410816987496448
score 15,812429

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