HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis

HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15) acts as a substrate receptor of E3 ligase complex, which plays a negative role in drought stress tolerance. However, whether and how HOS15 participates in controlling important transcriptional regulators remains largely unknown. Here, we repo...

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Autores: Zareen, Shah, Ali, Akhtar, Park, Junghoon, Kang, Sang-Mo, Lee, In-Jung, Pardo, José M., Yun, Dae-Jin, Xu, Zheng-Yi
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/384390
Acceso en línea:http://hdl.handle.net/10261/384390
https://api.elsevier.com/content/abstract/scopus_id/85216552186
Access Level:acceso abierto
Palabra clave:ABFs TFs
ABA and drought stress
Arabidopsis
DIL9
HOS15
proteasomal degradation
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spelling HOS15 impacts DIL9 protein stability during drought stress in ArabidopsisZareen, ShahAli, AkhtarPark, JunghoonKang, Sang-MoLee, In-JungPardo, José M.Yun, Dae-JinXu, Zheng-YiABFs TFsABA and drought stressArabidopsisDIL9HOS15proteasomal degradationHIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15) acts as a substrate receptor of E3 ligase complex, which plays a negative role in drought stress tolerance. However, whether and how HOS15 participates in controlling important transcriptional regulators remains largely unknown. Here, we report that HOS15 physically interacts with and tightly regulates DROUGHT-INDUCED LIKE 19 (DIL9) protein stability. Moreover, application of exogenous abscisic acid (ABA) stabilizes the interaction between DIL9 and HOS15, leading to ABA-induced proteasomal degradation of DIL9 by HOS15. Genetic analysis revealed that DIL9 functions downstream to HOS15 and that the drought tolerance of hos15-2 plants was impaired in dil9/hos15 double mutants. Notably, DIL9 is directly associated with the promoter regions of ABF transcription factors and facilitates their expression, which is pivotal in enhancing ABA-dependent drought tolerance. Collectively, these findings demonstrate that HOS15 consistently degrades DIL9 under normal condition, while stress (drought/ABA) promotes the DIL9 activity for binding to the promoter regions of ABFs and positively regulates their expression in response to dehydration.National Research Foundation of Korea. Grant Numbers: 2022R1A2C3004098, RS-2023-00239735 Bulgarian National Science Fund. Grant Number: КП06 ДВ/2 ЦС Ministry of Science and ICT, South Korea. Grant Number: RS-2024-00407469Peer reviewedJohn Wiley & SonsNational Research Foundation of KoreaBulgarian National Science FundMinistry of Science and ICT (Korea)Zareen, Shah [0000-0003-4419-8816]Ali, Akhtar [0000-0002-3666-4728]Park, Junghoon [0000-0002-7209-2990]Kang, Sang-Mo #NODATA##NODATA# Lee, In-JungPardo, José M. [0000-0003-4510-8624]Yun, Dae-Jin [0000-0002-3638-6043]Xu, Zheng-Yi [0000-0003-4256-304X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/384390https://api.elsevier.com/content/abstract/scopus_id/85216552186reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#RS‐2024‐004074692022R1A2C3004098RS‐2023‐00239735https://doi.org/10.1111/nph.20398Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3843902026-05-22T06:33:51Z
dc.title.none.fl_str_mv HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
title HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
spellingShingle HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
Zareen, Shah
ABFs TFs
ABA and drought stress
Arabidopsis
DIL9
HOS15
proteasomal degradation
title_short HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
title_full HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
title_fullStr HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
title_full_unstemmed HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
title_sort HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis
dc.creator.none.fl_str_mv Zareen, Shah
Ali, Akhtar
Park, Junghoon
Kang, Sang-Mo
Lee, In-Jung
Pardo, José M.
Yun, Dae-Jin
Xu, Zheng-Yi
author Zareen, Shah
author_facet Zareen, Shah
Ali, Akhtar
Park, Junghoon
Kang, Sang-Mo
Lee, In-Jung
Pardo, José M.
Yun, Dae-Jin
Xu, Zheng-Yi
author_role author
author2 Ali, Akhtar
Park, Junghoon
Kang, Sang-Mo
Lee, In-Jung
Pardo, José M.
Yun, Dae-Jin
Xu, Zheng-Yi
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Research Foundation of Korea
Bulgarian National Science Fund
Ministry of Science and ICT (Korea)
Zareen, Shah [0000-0003-4419-8816]
Ali, Akhtar [0000-0002-3666-4728]
Park, Junghoon [0000-0002-7209-2990]
Kang, Sang-Mo #NODATA#
#NODATA# Lee, In-Jung
Pardo, José M. [0000-0003-4510-8624]
Yun, Dae-Jin [0000-0002-3638-6043]
Xu, Zheng-Yi [0000-0003-4256-304X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv ABFs TFs
ABA and drought stress
Arabidopsis
DIL9
HOS15
proteasomal degradation
topic ABFs TFs
ABA and drought stress
Arabidopsis
DIL9
HOS15
proteasomal degradation
description HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15) acts as a substrate receptor of E3 ligase complex, which plays a negative role in drought stress tolerance. However, whether and how HOS15 participates in controlling important transcriptional regulators remains largely unknown. Here, we report that HOS15 physically interacts with and tightly regulates DROUGHT-INDUCED LIKE 19 (DIL9) protein stability. Moreover, application of exogenous abscisic acid (ABA) stabilizes the interaction between DIL9 and HOS15, leading to ABA-induced proteasomal degradation of DIL9 by HOS15. Genetic analysis revealed that DIL9 functions downstream to HOS15 and that the drought tolerance of hos15-2 plants was impaired in dil9/hos15 double mutants. Notably, DIL9 is directly associated with the promoter regions of ABF transcription factors and facilitates their expression, which is pivotal in enhancing ABA-dependent drought tolerance. Collectively, these findings demonstrate that HOS15 consistently degrades DIL9 under normal condition, while stress (drought/ABA) promotes the DIL9 activity for binding to the promoter regions of ABFs and positively regulates their expression in response to dehydration.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/384390
https://api.elsevier.com/content/abstract/scopus_id/85216552186
url http://hdl.handle.net/10261/384390
https://api.elsevier.com/content/abstract/scopus_id/85216552186
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
RS‐2024‐00407469
2022R1A2C3004098
RS‐2023‐00239735
https://doi.org/10.1111/nph.20398
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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