Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)

Polybrominated diphenyl ethers (PBDEs) are well-known endocrine disrupting chemicals (EDCs) identified as organic persistent pollutant (POPs). Their metabolites OH-BDE and MeO-BDE have been reported to be potentially more toxic than the postulated precursor PBDEs. BDE-47 is the one of the most predo...

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Detalles Bibliográficos
Autores: Sanz Landaluce, Jon, De Oro Carretero, Paloma
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/73077
Acceso en línea:https://hdl.handle.net/20.500.14352/73077
Access Level:acceso abierto
Palabra clave:543
Bioconcentration
Biotransformation
BDE-47
Metabolites
GC-MS-µECD
Zebrafish eleutheroembryos
Química analítica (Química)
2301 Química Analítica
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spelling Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)Sanz Landaluce, JonDe Oro Carretero, Paloma543BioconcentrationBiotransformationBDE-47MetabolitesGC-MS-µECDZebrafish eleutheroembryosQuímica analítica (Química)2301 Química AnalíticaPolybrominated diphenyl ethers (PBDEs) are well-known endocrine disrupting chemicals (EDCs) identified as organic persistent pollutant (POPs). Their metabolites OH-BDE and MeO-BDE have been reported to be potentially more toxic than the postulated precursor PBDEs. BDE-47 is the one of the most predominant congener of PBDEs in the environment by its high presence on the industrially used mixtures. In this study, the bioaccumulation and biotransformation of BDE-47 into its major metabolites is evaluated using zebrafish (Danio rerio) eleutheroembryos adapting a previously developed alternative method to bioconcentration official guideline OECD 305, which reduces dramatically the animal suffering, time and cost. For the simultaneous determination of BDE-47 and its metabolites in larvae and exposure medium and considering the polarity difference of the analytes and the small sample size, the development of an validated analytical method is a clue step to ensure quality results. In this study, an ultrasound-assisted extraction followed by a SPE dispersive clean-up step and GC-MS-μECD with a previous derivatization process was optimized and validated. , Bioconcentration factors (BCF) were calculated using first order one-compartment toxicokinetic model. The profiles found show rapid absorption in the first hours of larval development and great bioaccumulative capacity, finding BCF of 6631 and 44210 at nominal concentrations of 10 and 1 μg·L-1, respectively. Metabolization studies show increasing concentrations of the metabolites BDE-28, 2'-OH-BDE-28 and 5-MeO-BDE-47 throughout the exposure time. The results obtained show the feasibility of the method for bioaccumulation and opens the possibility of metabolic studies with zebrafish eleutheroembryos, which is a very underdeveloped field without official testing or regulation.John Wiley & Sons, LtdUniversidad Complutense de Madrid20232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/73077reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial 3.0 Españahttps://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/730772026-06-02T12:44:21Z
dc.title.none.fl_str_mv Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
title Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
spellingShingle Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
Sanz Landaluce, Jon
543
Bioconcentration
Biotransformation
BDE-47
Metabolites
GC-MS-µECD
Zebrafish eleutheroembryos
Química analítica (Química)
2301 Química Analítica
title_short Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
title_full Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
title_fullStr Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
title_full_unstemmed Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
title_sort Bioaccumulation and Biotransformation of BDE‐47 Using Zebrafish Eleutheroembryos (Danio rerio)
dc.creator.none.fl_str_mv Sanz Landaluce, Jon
De Oro Carretero, Paloma
author Sanz Landaluce, Jon
author_facet Sanz Landaluce, Jon
De Oro Carretero, Paloma
author_role author
author2 De Oro Carretero, Paloma
author2_role author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Bioconcentration
Biotransformation
BDE-47
Metabolites
GC-MS-µECD
Zebrafish eleutheroembryos
Química analítica (Química)
2301 Química Analítica
topic 543
Bioconcentration
Biotransformation
BDE-47
Metabolites
GC-MS-µECD
Zebrafish eleutheroembryos
Química analítica (Química)
2301 Química Analítica
description Polybrominated diphenyl ethers (PBDEs) are well-known endocrine disrupting chemicals (EDCs) identified as organic persistent pollutant (POPs). Their metabolites OH-BDE and MeO-BDE have been reported to be potentially more toxic than the postulated precursor PBDEs. BDE-47 is the one of the most predominant congener of PBDEs in the environment by its high presence on the industrially used mixtures. In this study, the bioaccumulation and biotransformation of BDE-47 into its major metabolites is evaluated using zebrafish (Danio rerio) eleutheroembryos adapting a previously developed alternative method to bioconcentration official guideline OECD 305, which reduces dramatically the animal suffering, time and cost. For the simultaneous determination of BDE-47 and its metabolites in larvae and exposure medium and considering the polarity difference of the analytes and the small sample size, the development of an validated analytical method is a clue step to ensure quality results. In this study, an ultrasound-assisted extraction followed by a SPE dispersive clean-up step and GC-MS-μECD with a previous derivatization process was optimized and validated. , Bioconcentration factors (BCF) were calculated using first order one-compartment toxicokinetic model. The profiles found show rapid absorption in the first hours of larval development and great bioaccumulative capacity, finding BCF of 6631 and 44210 at nominal concentrations of 10 and 1 μg·L-1, respectively. Metabolization studies show increasing concentrations of the metabolites BDE-28, 2'-OH-BDE-28 and 5-MeO-BDE-47 throughout the exposure time. The results obtained show the feasibility of the method for bioaccumulation and opens the possibility of metabolic studies with zebrafish eleutheroembryos, which is a very underdeveloped field without official testing or regulation.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/73077
url https://hdl.handle.net/20.500.14352/73077
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial 3.0 España
https://creativecommons.org/licenses/by-nc/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial 3.0 España
https://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons, Ltd
publisher.none.fl_str_mv John Wiley & Sons, Ltd
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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