In vitro self-organized mouse small intestinal epithelial monolayer protocol

Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell compositi...

ver descrição completa

Detalhes bibliográficos
Autores: Altay, Gizem, Batlle, Eduard, Fernández-Majada, Vanesa, Martínez Fraiz, Elena
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/155452
Acesso em linha:https://hdl.handle.net/2445/155452
Access Level:acceso abierto
Palavra-chave:Cèl·lules epitelials
Ratolins (Animals de laboratori)
Epithelial cells
Mice as laboratory animals
id ES_734ad576fb4bb387840c0f93b07c8fa2
oai_identifier_str oai:diposit.ub.edu:2445/155452
network_acronym_str ES
network_name_str España
repository_id_str
spelling In vitro self-organized mouse small intestinal epithelial monolayer protocolAltay, GizemBatlle, EduardFernández-Majada, VanesaMartínez Fraiz, ElenaCèl·lules epitelialsRatolins (Animals de laboratori)Epithelial cellsMice as laboratory animalsDeveloping protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell composition but did not prove self-renewing capacities. Here, we show a simple method to obtain mouse small intestine-derived epithelial monolayers organized into proliferative crypt-like domains, containing stem cells, and differentiated villus-like regions, closely resembling the in vivo cell composition and distribution. In addition, we adapted our model to a tissue culture format compatible with functional studies and prove close to physiological barrier properties of our in vitro epithelial monolayers. Thus, we have set-up a protocol to generate physiologically relevant intestinal epithelial monolayers to be employed in assays where independent access to both luminal and basolateral compartments is needed, such as drug absorption, intracellular trafficking and microbiome-epithelium interaction assays.Bio-protocol LLC2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/155452Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésBio-protocol, 2020, vol. 10, num. 3, p. e3514https://doi.org/10.21769/BioProtoc.3514info:eu-repo/grantAgreement/EC/H2020/647863(c) Altay et al., 2020info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1554522026-05-27T06:46:51Z
dc.title.none.fl_str_mv In vitro self-organized mouse small intestinal epithelial monolayer protocol
title In vitro self-organized mouse small intestinal epithelial monolayer protocol
spellingShingle In vitro self-organized mouse small intestinal epithelial monolayer protocol
Altay, Gizem
Cèl·lules epitelials
Ratolins (Animals de laboratori)
Epithelial cells
Mice as laboratory animals
title_short In vitro self-organized mouse small intestinal epithelial monolayer protocol
title_full In vitro self-organized mouse small intestinal epithelial monolayer protocol
title_fullStr In vitro self-organized mouse small intestinal epithelial monolayer protocol
title_full_unstemmed In vitro self-organized mouse small intestinal epithelial monolayer protocol
title_sort In vitro self-organized mouse small intestinal epithelial monolayer protocol
dc.creator.none.fl_str_mv Altay, Gizem
Batlle, Eduard
Fernández-Majada, Vanesa
Martínez Fraiz, Elena
author Altay, Gizem
author_facet Altay, Gizem
Batlle, Eduard
Fernández-Majada, Vanesa
Martínez Fraiz, Elena
author_role author
author2 Batlle, Eduard
Fernández-Majada, Vanesa
Martínez Fraiz, Elena
author2_role author
author
author
dc.subject.none.fl_str_mv Cèl·lules epitelials
Ratolins (Animals de laboratori)
Epithelial cells
Mice as laboratory animals
topic Cèl·lules epitelials
Ratolins (Animals de laboratori)
Epithelial cells
Mice as laboratory animals
description Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell composition but did not prove self-renewing capacities. Here, we show a simple method to obtain mouse small intestine-derived epithelial monolayers organized into proliferative crypt-like domains, containing stem cells, and differentiated villus-like regions, closely resembling the in vivo cell composition and distribution. In addition, we adapted our model to a tissue culture format compatible with functional studies and prove close to physiological barrier properties of our in vitro epithelial monolayers. Thus, we have set-up a protocol to generate physiologically relevant intestinal epithelial monolayers to be employed in assays where independent access to both luminal and basolateral compartments is needed, such as drug absorption, intracellular trafficking and microbiome-epithelium interaction assays.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/155452
url https://hdl.handle.net/2445/155452
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Bio-protocol, 2020, vol. 10, num. 3, p. e3514
https://doi.org/10.21769/BioProtoc.3514
info:eu-repo/grantAgreement/EC/H2020/647863
dc.rights.none.fl_str_mv (c) Altay et al., 2020
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Altay et al., 2020
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bio-protocol LLC
publisher.none.fl_str_mv Bio-protocol LLC
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410798672019456
score 15,301603

Registos relacionados