An Evolved 5 &apos

[EN] The results obtained in the present work could challenge the view of the role of the virus particle in the systemic transport of plant viruses. In this sense, we show that two different MPs are competent to systemically transport the AMV genome without the requirement of the virus particles, as...

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Autores: Villar-Álvarez, David, ELENA FITO, SANTIAGO FCO, Sánchez-Navarro, Jesús Á., Pallás Benet, Vicente|||0000-0003-4954-989X
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/196929
Acceso en línea:https://riunet.upv.es/handle/10251/196929
Access Level:acceso abierto
Palabra clave:Cell-to-cell and systemic movement
Virus particles
AMV model system
BMV
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repository_id_str
dc.title.none.fl_str_mv An Evolved 5 &apos
Untranslated Region of Alfalfa Mosaic Virus Allows the RNA Transport of Movement-Defective Variants
title An Evolved 5 &apos
spellingShingle An Evolved 5 &apos
Villar-Álvarez, David
Cell-to-cell and systemic movement
Virus particles
AMV model system
BMV
title_short An Evolved 5 &apos
title_full An Evolved 5 &apos
title_fullStr An Evolved 5 &apos
title_full_unstemmed An Evolved 5 &apos
title_sort An Evolved 5 &apos
dc.creator.none.fl_str_mv Villar-Álvarez, David
ELENA FITO, SANTIAGO FCO
Sánchez-Navarro, Jesús Á.
Pallás Benet, Vicente|||0000-0003-4954-989X
author Villar-Álvarez, David
author_facet Villar-Álvarez, David
ELENA FITO, SANTIAGO FCO
Sánchez-Navarro, Jesús Á.
Pallás Benet, Vicente|||0000-0003-4954-989X
author_role author
author2 ELENA FITO, SANTIAGO FCO
Sánchez-Navarro, Jesús Á.
Pallás Benet, Vicente|||0000-0003-4954-989X
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Generalitat Valenciana
Agencia Estatal de Investigación
European Regional Development Fund
Universitat Politècnica de València
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Cell-to-cell and systemic movement
Virus particles
AMV model system
BMV
topic Cell-to-cell and systemic movement
Virus particles
AMV model system
BMV
description [EN] The results obtained in the present work could challenge the view of the role of the virus particle in the systemic transport of plant viruses. In this sense, we show that two different MPs are competent to systemically transport the AMV genome without the requirement of the virus particles, as reported for viruses lacking a CP (e.g., Umbravirus). Although the coat protein (CP) has a relevant role in the long-distance movement of alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), its precise function is not fully understood. Previous results showed that a specific interaction between the C termini of the movement protein (MP) and the cognate CP is required for systemic transport. Thus, we have performed a compensatory evolution experiment using an AMV RNA3 derivative defective in long-distance transport that carries a BMV MP lacking the C-terminal 48 residues and unable to interact with the AMV CP. After several passages, five independent evolution lineages were able to move long distance. The analysis of the viral RNA of these lineages showed the presence of three different modifications located exclusively at the 5 ' untranslated region (5 ' UTR). The three evolved 5 ' UTR variants accumulated comparable levels of viral RNA and CP but reduced the accumulation of virus particles and the affinity between the 5 ' UTR and the AMV CP. In addition, the evolved 5 ' UTR increased cell-to-cell transport for both the AMV RNA3 carrying the BMV MP and that carrying the AMV MP. Finally, the evolved 5 ' UTRs allowed the systemic transport of an AMV RNA3 carrying a CP mutant defective in virus particles and increased the systemic transport of several AMV RNA3 derivatives carrying different viral MPs associated with the 30K superfamily. Altogether, our findings indicate that virus particles are not required for the systemic transport of AMV but also that BMV MP is competent for the short- and long-distance transport without the interaction with the CP. IMPORTANCE The results obtained in the present work could challenge the view of the role of the virus particle in the systemic transport of plant viruses. In this sense, we show that two different MPs are competent to systemically transport the AMV genome without the requirement of the virus particles, as reported for viruses lacking a CP (e.g., Umbravirus). The incapability of the viral MP to interact with the CP triggered virus variants that evolved to reduce the formation of virus particles, probably to increase the accessibility of the MP to the viral progeny. Our results point to the idea that virus particles would not be necessary for the viral systemic transport but would be necessary for vector virus transmission. This idea is reinforced by the observation that heterologous MPs also increased the systemic transport of the AMV constructs that have reduced encapsidation capabilities.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-11-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/196929
url https://riunet.upv.es/handle/10251/196929
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-103998GB-I00 PAPEL DEL CODIGO EPIGENOMICO DEL HUESPED EN LA EVOLUCION DE LAS POBLACIONES VIRALES
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2019%2F012
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115571RB-I00 LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2015%2F010 Interacciones RNA-proteína y proteína-proteína en procesos de desarrollo y patogénesis mediados por virus y agentes subvirales en cultivos de interés Agronómico. (ACRONIMO: RNAPROT)
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling An Evolved 5 &aposUntranslated Region of Alfalfa Mosaic Virus Allows the RNA Transport of Movement-Defective VariantsVillar-Álvarez, DavidELENA FITO, SANTIAGO FCOSánchez-Navarro, Jesús Á.Pallás Benet, Vicente|||0000-0003-4954-989XCell-to-cell and systemic movementVirus particlesAMV model systemBMV[EN] The results obtained in the present work could challenge the view of the role of the virus particle in the systemic transport of plant viruses. In this sense, we show that two different MPs are competent to systemically transport the AMV genome without the requirement of the virus particles, as reported for viruses lacking a CP (e.g., Umbravirus). Although the coat protein (CP) has a relevant role in the long-distance movement of alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), its precise function is not fully understood. Previous results showed that a specific interaction between the C termini of the movement protein (MP) and the cognate CP is required for systemic transport. Thus, we have performed a compensatory evolution experiment using an AMV RNA3 derivative defective in long-distance transport that carries a BMV MP lacking the C-terminal 48 residues and unable to interact with the AMV CP. After several passages, five independent evolution lineages were able to move long distance. The analysis of the viral RNA of these lineages showed the presence of three different modifications located exclusively at the 5 ' untranslated region (5 ' UTR). The three evolved 5 ' UTR variants accumulated comparable levels of viral RNA and CP but reduced the accumulation of virus particles and the affinity between the 5 ' UTR and the AMV CP. In addition, the evolved 5 ' UTR increased cell-to-cell transport for both the AMV RNA3 carrying the BMV MP and that carrying the AMV MP. Finally, the evolved 5 ' UTRs allowed the systemic transport of an AMV RNA3 carrying a CP mutant defective in virus particles and increased the systemic transport of several AMV RNA3 derivatives carrying different viral MPs associated with the 30K superfamily. Altogether, our findings indicate that virus particles are not required for the systemic transport of AMV but also that BMV MP is competent for the short- and long-distance transport without the interaction with the CP. IMPORTANCE The results obtained in the present work could challenge the view of the role of the virus particle in the systemic transport of plant viruses. In this sense, we show that two different MPs are competent to systemically transport the AMV genome without the requirement of the virus particles, as reported for viruses lacking a CP (e.g., Umbravirus). The incapability of the viral MP to interact with the CP triggered virus variants that evolved to reduce the formation of virus particles, probably to increase the accessibility of the MP to the viral progeny. Our results point to the idea that virus particles would not be necessary for the viral systemic transport but would be necessary for vector virus transmission. This idea is reinforced by the observation that heterologous MPs also increased the systemic transport of the AMV constructs that have reduced encapsidation capabilities.We are grateful to Lorena Corachan for excellent technical support. This work was supported by grants PID2020-115571RB-I00 and PID2019-103998GB-I00 from the Spanish MCIN/AEI/10.13039/501100011033 granting agency-FEDER (V.P. and S.F.E., respectively) and PROMETEO/2015/010 and PROMETEO2019/012 from the Generalitat Valenciana (V.P. and S.F.E., respectively).American Society for MicrobiologyInstituto Universitario Mixto de Biología Molecular y Celular de PlantasGeneralitat ValencianaAgencia Estatal de InvestigaciónEuropean Regional Development FundUniversitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-11-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/196929reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-103998GB-I00 PAPEL DEL CODIGO EPIGENOMICO DEL HUESPED EN LA EVOLUCION DE LAS POBLACIONES VIRALESGeneralitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2019%2F012Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115571RB-I00 LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTASGeneralitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2015%2F010 Interacciones RNA-proteína y proteína-proteína en procesos de desarrollo y patogénesis mediados por virus y agentes subvirales en cultivos de interés Agronómico. (ACRONIMO: RNAPROT)open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1969292026-06-13T07:49:27Z
score 15,301603

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