Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases

Vilà Quintana, Laura; Fort, Esther; Pardo, Laura; Albiol Quer, Maria T.; Ortiz, Maria Rosa; Capdevila, Montserrat; Feliu, Anna; Bahí, Anna; Llirós, Marc; García Velasco, Adelaida; Morell Ginestà, Mireia; Laquente, Berta; Pozas, Débora; Moreno Aguado, Víctor; Garcia Gil, Librado Jesús; Duell, Eric Jeffrey; Pimenoff, Ville Nikolai; Carreras Torres, Robert; Aldeguer, Xavier

Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases

Individuals with pancreatic-related health conditions usually show lower diversity and different composition of bacterial and viral species between the gut and oral microbiomes compared to healthy individuals. We performed a thorough microbiome analysis, using deep shotgun sequencing of stool and sa...

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Autores:	Vilà Quintana, Laura, Fort, Esther, Pardo, Laura, Albiol Quer, Maria T., Ortiz, Maria Rosa, Capdevila, Montserrat, Feliu, Anna, Bahí, Anna, Llirós, Marc, García Velasco, Adelaida, Morell Ginestà, Mireia, Laquente, Berta, Pozas, Débora, Moreno Aguado, Víctor, Garcia Gil, Librado Jesús, Duell, Eric Jeffrey, Pimenoff, Ville Nikolai, Carreras Torres, Robert, Aldeguer, Xavier
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2024
País:	España
Institución:	Universidad de Barcelona
Repositorio:	Dipòsit Digital de la UB
OAI Identifier:	oai:diposit.ub.edu:2445/216399
Acceso en línea:	https://hdl.handle.net/2445/216399
Access Level:	acceso abierto
Palabra clave:	Metagenòmica Càncer de pàncrees Metagenomics Pancreas cancer

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spelling	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic DiseasesVilà Quintana, LauraFort, EstherPardo, LauraAlbiol Quer, Maria T.Ortiz, Maria RosaCapdevila, MontserratFeliu, AnnaBahí, AnnaLlirós, MarcGarcía Velasco, AdelaidaMorell Ginestà, MireiaLaquente, BertaPozas, DéboraMoreno Aguado, VíctorGarcia Gil, Librado JesúsDuell, Eric JeffreyPimenoff, Ville NikolaiCarreras Torres, RobertAldeguer, XavierMetagenòmicaCàncer de pàncreesMetagenomicsPancreas cancerIndividuals with pancreatic-related health conditions usually show lower diversity and different composition of bacterial and viral species between the gut and oral microbiomes compared to healthy individuals. We performed a thorough microbiome analysis, using deep shotgun sequencing of stool and saliva samples obtained from patients with chronic pancreatitis (CP), pancreatic ductal adenocarcinoma (PDAC), and healthy controls (HCs).We observed similar microbiota composition at the species level in both the gut and oral samples in PDAC patients compared to HCs, among which the most distinctive finding was that the abundance of oral-originated Fusobacterium nucleatum species did not differ between the oral and the gut samples. Moreover, comparing PDAC patients with HCs, Klebsiella oxytoca was significantly more abundant in the stool samples of PDAC patients, while Streptococcus spp. showed higher abundance in both the oral and stool samples of PDAC patients. Finally, the most important finding was the distinctive gut phage-bacterial interactome pattern among PDAC patients. CrAssphages, particularly Blohavirus, showed mutual exclusion with K. oxytoca species, while Burzaovirus showed co-occurrence with Enterobacteriaceae spp., which have been shown to be capable of inducing DNA damage in human pancreatic cells ex vivo. The interactome findings warrant further mechanistic studies, as our findings may provide new insights into developing microbiota-based diagnostic and therapeutic methods for pancreatic diseases.MDPI AG2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/216399Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms252010988International Journal of Molecular Sciences, 2024, vol. 25, num. 20, p. 10988https://doi.org/10.3390/ijms252010988cc by (c) Vilà Quintana, Laura et al., 2024http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2163992026-05-27T06:46:51Z
dc.title.none.fl_str_mv	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
title	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
spellingShingle	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases Vilà Quintana, Laura Metagenòmica Càncer de pàncrees Metagenomics Pancreas cancer
title_short	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
title_full	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
title_fullStr	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
title_full_unstemmed	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
title_sort	Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
dc.creator.none.fl_str_mv	Vilà Quintana, Laura Fort, Esther Pardo, Laura Albiol Quer, Maria T. Ortiz, Maria Rosa Capdevila, Montserrat Feliu, Anna Bahí, Anna Llirós, Marc García Velasco, Adelaida Morell Ginestà, Mireia Laquente, Berta Pozas, Débora Moreno Aguado, Víctor Garcia Gil, Librado Jesús Duell, Eric Jeffrey Pimenoff, Ville Nikolai Carreras Torres, Robert Aldeguer, Xavier
author	Vilà Quintana, Laura
author_facet	Vilà Quintana, Laura Fort, Esther Pardo, Laura Albiol Quer, Maria T. Ortiz, Maria Rosa Capdevila, Montserrat Feliu, Anna Bahí, Anna Llirós, Marc García Velasco, Adelaida Morell Ginestà, Mireia Laquente, Berta Pozas, Débora Moreno Aguado, Víctor Garcia Gil, Librado Jesús Duell, Eric Jeffrey Pimenoff, Ville Nikolai Carreras Torres, Robert Aldeguer, Xavier
author_role	author
author2	Fort, Esther Pardo, Laura Albiol Quer, Maria T. Ortiz, Maria Rosa Capdevila, Montserrat Feliu, Anna Bahí, Anna Llirós, Marc García Velasco, Adelaida Morell Ginestà, Mireia Laquente, Berta Pozas, Débora Moreno Aguado, Víctor Garcia Gil, Librado Jesús Duell, Eric Jeffrey Pimenoff, Ville Nikolai Carreras Torres, Robert Aldeguer, Xavier
author2_role	author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Metagenòmica Càncer de pàncrees Metagenomics Pancreas cancer
topic	Metagenòmica Càncer de pàncrees Metagenomics Pancreas cancer
description	Individuals with pancreatic-related health conditions usually show lower diversity and different composition of bacterial and viral species between the gut and oral microbiomes compared to healthy individuals. We performed a thorough microbiome analysis, using deep shotgun sequencing of stool and saliva samples obtained from patients with chronic pancreatitis (CP), pancreatic ductal adenocarcinoma (PDAC), and healthy controls (HCs).We observed similar microbiota composition at the species level in both the gut and oral samples in PDAC patients compared to HCs, among which the most distinctive finding was that the abundance of oral-originated Fusobacterium nucleatum species did not differ between the oral and the gut samples. Moreover, comparing PDAC patients with HCs, Klebsiella oxytoca was significantly more abundant in the stool samples of PDAC patients, while Streptococcus spp. showed higher abundance in both the oral and stool samples of PDAC patients. Finally, the most important finding was the distinctive gut phage-bacterial interactome pattern among PDAC patients. CrAssphages, particularly Blohavirus, showed mutual exclusion with K. oxytoca species, while Burzaovirus showed co-occurrence with Enterobacteriaceae spp., which have been shown to be capable of inducing DNA damage in human pancreatic cells ex vivo. The interactome findings warrant further mechanistic studies, as our findings may provide new insights into developing microbiota-based diagnostic and therapeutic methods for pancreatic diseases.
publishDate	2024
dc.date.none.fl_str_mv	2024
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/216399
url	https://hdl.handle.net/2445/216399
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.3390/ijms252010988 International Journal of Molecular Sciences, 2024, vol. 25, num. 20, p. 10988 https://doi.org/10.3390/ijms252010988
dc.rights.none.fl_str_mv	cc by (c) Vilà Quintana, Laura et al., 2024 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc by (c) Vilà Quintana, Laura et al., 2024 http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	MDPI AG
publisher.none.fl_str_mv	MDPI AG
dc.source.none.fl_str_mv	Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona
instname_str	Universidad de Barcelona
reponame_str	Dipòsit Digital de la UB
collection	Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.81155

Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases

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