The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities

We present here the characterization of a new gene family, awr, found in all sequenced Ralstonia solanacearum strains and in other bacterial pathogens. We demonstrate that the five paralogues in strain GMI1000 encode type III-secreted effectors and that deletion of all awr genes severely impairs its...

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Autores: Solé Castellvi, Montserrat, Popa, Crina Mihaela, Mith, Oriane, Sohn, Kee Hoon, Jones, Jonathan D. G., Deslandes, Laurent, Valls i Matheu, Marc
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/27682
Acesso em linha:https://hdl.handle.net/2445/27682
Access Level:acceso abierto
Palavra-chave:Genètica
Gens
Bacteris patògens
Plantes
Microorganismes patògens
Virulència (Microbiologia)
Genetics
Pathogenic bacteria
Plants
Pathogenic microorganisms
Virulence (Microbiology)
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repository_id_str
spelling The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activitiesSolé Castellvi, MontserratPopa, Crina MihaelaMith, OrianeSohn, Kee HoonJones, Jonathan D. G.Deslandes, LaurentValls i Matheu, MarcGenèticaGensBacteris patògensPlantesMicroorganismes patògensVirulència (Microbiologia)GeneticsGensPathogenic bacteriaPlantsPathogenic microorganismsVirulence (Microbiology)We present here the characterization of a new gene family, awr, found in all sequenced Ralstonia solanacearum strains and in other bacterial pathogens. We demonstrate that the five paralogues in strain GMI1000 encode type III-secreted effectors and that deletion of all awr genes severely impairs its capacity to multiply in natural host plants. Complementation studies show that the AWR (alanine-tryptophanarginine tryad) effectors display some functional redundancy, although AWR2 is the major contributor to virulence. In contrast, the strain devoid of all awr genes (¿awr1-5) exhibits enhanced pathogenicity on Arabidopsis plants. A gain-of-function approach expressing AWR in Pseudomonas syringae pv. tomato DC3000 proves that this is likely due to effector recognition, because AWR5 and AWR4 restrict growth of this bacterium in Arabidopsis. Transient overexpression of AWR in nonhost tobacco species caused macroscopic cell death to varying extents, which, in the case of AWR5, shows characteristics of a typical hypersensitive response. Our work demonstrates that AWR, which show no similarity to any protein with known function, can specify either virulence or avirulence in the interaction of R. solanacearum with its plant hosts.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/27682Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1094/MPMI-12-11-0321Molecular Plant-Microbe Interactions, 2012, vol. 25, num. 7, p. 941-953http://dx.doi.org/10.1094/MPMI-12-11-0321CC0 American Phytopathological Society, 2012http://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/276822026-05-27T06:46:51Z
dc.title.none.fl_str_mv The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
title The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
spellingShingle The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
Solé Castellvi, Montserrat
Genètica
Gens
Bacteris patògens
Plantes
Microorganismes patògens
Virulència (Microbiologia)
Genetics
Gens
Pathogenic bacteria
Plants
Pathogenic microorganisms
Virulence (Microbiology)
title_short The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
title_full The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
title_fullStr The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
title_full_unstemmed The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
title_sort The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities
dc.creator.none.fl_str_mv Solé Castellvi, Montserrat
Popa, Crina Mihaela
Mith, Oriane
Sohn, Kee Hoon
Jones, Jonathan D. G.
Deslandes, Laurent
Valls i Matheu, Marc
author Solé Castellvi, Montserrat
author_facet Solé Castellvi, Montserrat
Popa, Crina Mihaela
Mith, Oriane
Sohn, Kee Hoon
Jones, Jonathan D. G.
Deslandes, Laurent
Valls i Matheu, Marc
author_role author
author2 Popa, Crina Mihaela
Mith, Oriane
Sohn, Kee Hoon
Jones, Jonathan D. G.
Deslandes, Laurent
Valls i Matheu, Marc
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Genètica
Gens
Bacteris patògens
Plantes
Microorganismes patògens
Virulència (Microbiologia)
Genetics
Gens
Pathogenic bacteria
Plants
Pathogenic microorganisms
Virulence (Microbiology)
topic Genètica
Gens
Bacteris patògens
Plantes
Microorganismes patògens
Virulència (Microbiologia)
Genetics
Gens
Pathogenic bacteria
Plants
Pathogenic microorganisms
Virulence (Microbiology)
description We present here the characterization of a new gene family, awr, found in all sequenced Ralstonia solanacearum strains and in other bacterial pathogens. We demonstrate that the five paralogues in strain GMI1000 encode type III-secreted effectors and that deletion of all awr genes severely impairs its capacity to multiply in natural host plants. Complementation studies show that the AWR (alanine-tryptophanarginine tryad) effectors display some functional redundancy, although AWR2 is the major contributor to virulence. In contrast, the strain devoid of all awr genes (¿awr1-5) exhibits enhanced pathogenicity on Arabidopsis plants. A gain-of-function approach expressing AWR in Pseudomonas syringae pv. tomato DC3000 proves that this is likely due to effector recognition, because AWR5 and AWR4 restrict growth of this bacterium in Arabidopsis. Transient overexpression of AWR in nonhost tobacco species caused macroscopic cell death to varying extents, which, in the case of AWR5, shows characteristics of a typical hypersensitive response. Our work demonstrates that AWR, which show no similarity to any protein with known function, can specify either virulence or avirulence in the interaction of R. solanacearum with its plant hosts.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/27682
url https://hdl.handle.net/2445/27682
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1094/MPMI-12-11-0321
Molecular Plant-Microbe Interactions, 2012, vol. 25, num. 7, p. 941-953
http://dx.doi.org/10.1094/MPMI-12-11-0321
dc.rights.none.fl_str_mv CC0 American Phytopathological Society, 2012
http://creativecommons.org/publicdomain/zero/1.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC0 American Phytopathological Society, 2012
http://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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