Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1

Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, w...

Descripción completa

Detalles Bibliográficos
Autores: Juárez, Antonio, Villa Torrecilla, Juan Antonio, Lanza, Val F., Lázaro, Beatriz, Cruz, Fernando de la|||0000-0003-4758-6857, Álvarez, Héctor M., Moncalián Montes, Gabriel|||0000-0002-3007-6490
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/11856
Acceso en línea:http://hdl.handle.net/10902/11856
Access Level:acceso abierto
id ES_72645eca380a607cd511f006fc9fb5db
oai_identifier_str oai:repositorio.unican.es:10902/11856
network_acronym_str ES
network_name_str España
repository_id_str
spelling Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1Juárez, AntonioVilla Torrecilla, Juan AntonioLanza, Val F.Lázaro, BeatrizCruz, Fernando de la|||0000-0003-4758-6857Álvarez, Héctor M.Moncalián Montes, Gabriel|||0000-0002-3007-6490Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMPdependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.BioMed CentralUniversidad de Cantabria20172017-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/11856Microbial Cell Factories 2017 16: 35reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/118562026-06-02T12:39:31Z
dc.title.none.fl_str_mv Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
spellingShingle Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
Juárez, Antonio
title_short Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_fullStr Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full_unstemmed Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_sort Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
dc.creator.none.fl_str_mv Juárez, Antonio
Villa Torrecilla, Juan Antonio
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la|||0000-0003-4758-6857
Álvarez, Héctor M.
Moncalián Montes, Gabriel|||0000-0002-3007-6490
author Juárez, Antonio
author_facet Juárez, Antonio
Villa Torrecilla, Juan Antonio
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la|||0000-0003-4758-6857
Álvarez, Héctor M.
Moncalián Montes, Gabriel|||0000-0002-3007-6490
author_role author
author2 Villa Torrecilla, Juan Antonio
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la|||0000-0003-4758-6857
Álvarez, Héctor M.
Moncalián Montes, Gabriel|||0000-0002-3007-6490
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
description Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMPdependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10902/11856
url http://hdl.handle.net/10902/11856
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
http://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Microbial Cell Factories 2017 16: 35
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410732392579072
score 15,300719

Similares en LA Referencia