Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase

This article belongs to the Section Molecular Genetics and Genomics.

Detalles Bibliográficos
Autores: Carvalho, Gustavo, Guerra, Susana, Martínez-Jiménez, María I., Blanco Dávila, Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/396697
Acceso en línea:http://hdl.handle.net/10261/396697
Access Level:acceso abierto
Palabra clave:PrimPol
Mouse PrimPol
DNA primase
DNA replication
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spelling Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA PrimaseCarvalho, GustavoGuerra, SusanaMartínez-Jiménez, María I.Blanco Dávila, LuisPrimPolMouse PrimPolDNA primaseDNA replicationThis article belongs to the Section Molecular Genetics and Genomics.The human PrimPol counteracts DNA replication stress by repriming DNA synthesis when fork progression is hindered by UV light or hydroxyurea treatment, or by encountering complex DNA structures, such as G-quadruplexes, R-loops, or interstrand crosslinks. The Mus musculus PrimPol (MmPrimPol) shares a high degree of amino acid similarity with its human ortholog; however, as shown here, MmPrimPol exhibits a more powerful primase activity compared to the human enzyme. Such a robust primase activity relies on an enhanced ability to bind the 5′ site nucleotide, and consequently to form initial dimers and further mature primers. Additionally, a shorter linker between the AEP core and the Zn finger domain (ZnFD) in the murine homolog likely promotes a constitutive closing of these domains into a primase-ready configuration. Consequently, a reinforced close configuration of the ZnFD would explain why MmPrimPol has a more robust primase, but a very limited DNA polymerization on an existing primer.This research was funded by MINECO, grant number BFU2015-65880-P and MCIN/AEI/10.13039/501100011033, by “ERDF A way of making Europe”, by the “European Union Next Generation EU/PRTR, grant numbers PGC2018-093576-B-C21 and PID2021-125966OB-I00 to L.B. G.C. was recipient of a predoctoral fellowship from the Brazilian National Council for Scientific and Technological Development (249545/2013-6).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de de Economía, Comercio y Empresa (España)European CommissionConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Carvalho, Gustavo [0000-0002-3827-4619]Martínez-Jiménez, María I. [0000-0002-7653-2877]Blanco Dávila, Luis [0000-0001-5558-4435]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/396697reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//BFU2015-65880-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-093576-B-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125966OB-I00https://doi.org/10.3390/ijms26146947Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3966972026-05-22T06:33:51Z
dc.title.none.fl_str_mv Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
title Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
spellingShingle Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
Carvalho, Gustavo
PrimPol
Mouse PrimPol
DNA primase
DNA replication
title_short Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
title_full Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
title_fullStr Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
title_full_unstemmed Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
title_sort Mouse PrimPol Outperforms Its Human Counterpart as a Robust DNA Primase
dc.creator.none.fl_str_mv Carvalho, Gustavo
Guerra, Susana
Martínez-Jiménez, María I.
Blanco Dávila, Luis
author Carvalho, Gustavo
author_facet Carvalho, Gustavo
Guerra, Susana
Martínez-Jiménez, María I.
Blanco Dávila, Luis
author_role author
author2 Guerra, Susana
Martínez-Jiménez, María I.
Blanco Dávila, Luis
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de de Economía, Comercio y Empresa (España)
European Commission
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Carvalho, Gustavo [0000-0002-3827-4619]
Martínez-Jiménez, María I. [0000-0002-7653-2877]
Blanco Dávila, Luis [0000-0001-5558-4435]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PrimPol
Mouse PrimPol
DNA primase
DNA replication
topic PrimPol
Mouse PrimPol
DNA primase
DNA replication
description This article belongs to the Section Molecular Genetics and Genomics.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/396697
url http://hdl.handle.net/10261/396697
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/MINECO//BFU2015-65880-P
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-093576-B-C21
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-125966OB-I00
https://doi.org/10.3390/ijms26146947
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
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instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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